FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_36_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_36_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515149
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTTTTGG82261.5968195609425622No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG42030.8158804540045697No Hit
CCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGAC40450.7852097160239077No Hit
CCCAAAAATGGATCATTACAAAATTGCCTGTTAGGCAGCTCTCCCTAGCA37620.7302741536914563No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT36040.6996034157107944No Hit
GCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTTTTGGGT33590.652044359981287No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT31160.6048735414414083No Hit
GTGCTATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGATCCAT31160.6048735414414083No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGCCTGTTAGGCAGCTCTC30340.5889558166666343No Hit
ATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGT26450.5134436832838655No Hit
GTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA25360.492284756449105No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT24720.47986116638098875No Hit
CCCAAAAATGGATCATTACAAAATTGACACCCAAAAAGTCACTGGGCCTC24490.4753964387002595No Hit
GCCTAACAGGCAATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTC23320.45268456310698457No Hit
CCCTAGCATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTG21640.42007263917817955No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC20980.40726081192043473No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC18910.3670782627938713No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG18790.3647488396560995No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGACACCCAAAAAGTCACT18530.35970175619092726No Hit
CACCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCG18250.3542664355361264No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA18240.3540723169413121No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT17550.3406781338991243No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA17250.3348545760546948No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT16710.3243721719347218No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG15260.29622497568664596No Hit
ATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTCAATGTGTCCAGC14920.2896249434629593No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA14590.28321902983408687No Hit
GTCCAATGTGCTATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAAT14210.27584252323114283No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT13600.26400128894746955No Hit
CCCAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTT13520.262448340188955No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC13470.2614777472148835No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT13110.25448947780156805No Hit
ATGCTAGGGAGAGCTGCCTACAATGCTAGGGAGAGCTGCCTAACAGGCAA12920.2508012245000961No Hit
CTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC12860.24963651293121017No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT12750.2475012083882527No Hit
TGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC12180.23643644848383671No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC12060.23410702534606495No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG11990.2327481951823647No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC11960.23216583939792176No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT11820.2294481790705213No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC11450.22226579106239164No Hit
CTATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGATCCATTTT11420.2216834352779487No Hit
CAATCAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCAT11290.21915989354536258No Hit
ATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA11250.21838341916610532No Hit
CAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTTTT11140.21624811462314786No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA10970.2129480985113045No Hit
GCCTACAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCA10420.2022715757965171No Hit
GGTTCACAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCC10300.19994215265874532No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC9990.19392447621950154No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA9900.1921774088661727No Hit
CCAATGTGCTATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGA9780.18984798572840092No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT9570.18577149523730027No Hit
CATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTGCCTGTT9540.18518913945285734No Hit
AGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTTTTGGGTGTA9490.18421854647878577No Hit
GCCCACAATACACCCAAAAATGGATCATTACAAAATTGCCTGTTAGGCAG9460.1836361906943428No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA9410.18266559772027122No Hit
TTACAAAATTGCCTGTTAGGCAGCTCTCCCTAGCATTGTAGGCAGCTCTC9270.1799479373928708No Hit
ATTTTTGGGTGTATTGTGGGCTCAATGTGTCCAGCAATTTTGTAATGATC9250.17955970020324216No Hit
ATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCG9180.17820087003954196No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA9080.1762596840913988No Hit
TGATAGAGCCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTG9000.17470673533288428No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT8540.16577727997142575No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC8460.16422433121291122No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC8230.15975960353218194No Hit
CCAATATTCCAGGCACCAATGCTAGGGAGAGCTGCCTAACAGGCAATTTT8090.15704194320478151No Hit
GGCTCAATGTGTCCAATGTGCTATGAGGCCCAGTGACTTTTTGGGTGTCA8090.15704194320478151No Hit
CACCAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATT8060.15645958742033858No Hit
GGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGAT8010.15548899444626701No Hit
CCCAAAAATGGATCATTACAAAATTGATGGATCATTACAAAATTGCTGGA7690.14927719941220888No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC7690.14927719941220888No Hit
CCAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTTT7650.1485007250329516No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC7640.1483066064381373No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT7560.14675365767962278No Hit
CTACAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATT7520.14597718330036552No Hit
TTACAAAATTGATGGATCATTACAAAATTGCTGGACACATTGAGCCCACA7480.14520070892110826No Hit
CAATGTGTCCAATGTGCTATGAGGCCCAGTGACTTTTTGGGTGTCAATTT7450.14461835313666532No Hit
GCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTT7430.1442301159470367No Hit
AGCCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTT7410.14384187875740806No Hit
ATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTCAATGTGTCCAAT7400.14364776016259373No Hit
ATTTTGTAATGATCCATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGG7400.14364776016259373No Hit
GTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAA7350.14267716718852216No Hit
CAATAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATT7330.14228892999889353No Hit
CCACAATACACCCAAAAATGGATCATTACAAAATTGCCTGTTAGGCAGCT7280.14131833702482194No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC7250.140735981240379No Hit
ATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTCAATGTGTCCAGG7240.14054186264556467No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG7070.1372418465337213No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGATGGATCATTACAAAAT7050.13685360934409266No Hit
ATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTCAATGCTAGGGAG6940.13471830480113522No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA6850.13297123744780637No Hit
TTACAAAATTGACACCCAAAAAGTCACTGGGCCTCATAGCACATTGGACA6760.1312241700944775No Hit
GGTTCAAACAGATGCGCAAACAGGTTCACAATGCTAGGGAGAGCTGCCTA6660.12928298414633435No Hit
ACATTGGACACATTGAGCCCACAATACACCCAAAAATGGATCATTACAAA6640.12889474695670572No Hit
GCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGATCCATTTTTGGGTGT6610.12831239117226279No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT6570.12753591679300552No Hit
ATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGATCCATTTTTG6520.12656532381893393No Hit
GGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCT6520.12656532381893393No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTAACAGGCAATTT6510.12637120522411963No Hit
CCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG6360.12345942630190489No Hit
ATTTTTGGGTGTATTGTGGGCTCAATGCTAGGGAGAGCTGCCTAACAGGC6310.12248883332783331No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT6230.12093588456931878No Hit
TTACAAAATTGCTGGACACATTGAGCCCACAATACACCCAAAAATGGATC6230.12093588456931878No Hit
GTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCT6200.12035352878487582No Hit
GATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC6090.11821822424191836No Hit
CTTTTTGGGTGTCAATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGC6040.11724763126784678No Hit
ATTTTTGGGTGTATTGTGGGCTCAATGTGTCCAATGTGCTATGAGGCCCA6010.11666527548340384No Hit
CAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCT5940.11530644531970362No Hit
GCTATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGATCCATTT5890.11433585234563202No Hit
TTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTA5880.11414173375081772No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT5820.11297702218193183No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT5770.11200642920786025No Hit
CATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTGACACCC5700.11064759904416005No Hit
CCCTAGCATTGGTGCCTGGACACATTGAGCCCACAATACACCCAAAAATG5630.10928876888045982No Hit
AAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAA5610.10890053169083119No Hit
AGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGA5600.10870641309601688No Hit
GTATTGTGGGCTCAATGTGTCCAGCAATTTTGTAATGATCCATCAATTTT5560.10792993871675961No Hit
TTACAAAATTGCCTGTTAGGCAGCTCTCCCTAGCATTGAGCCCACAATAC5540.10754170152713098No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA5540.10754170152713098No Hit
GTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGA5490.10657110855305942No Hit
GCCCACAATACACCCAAAAATGGATCATTACAAAATTGACACCCAAAAAG5440.10560051557898782No Hit
ATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTT5360.1040475668204733No Hit
ATTGTGGGCTCAATGTGTCCAGCAATTTTGTAATGATCCATCAATTTTGT5320.10327109244121603No Hit
TCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACAC5250.10191226227751582No Hit
GGTGTATTGTGGGCTCAATGTGTCCAGCAATTTTGTAATGATCCATCAAT5240.10171814368270152No Hit
ATTGTGGGCTCAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATG5230.10152402508788719No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTGT6750.096.666672
AAGGGTA750.096.666672
TGTTGTC7000.093.214283
GGGTAGT2000.090.6251
TTATATT259.384144E-487.04
CCTGTTG7650.085.294111
GGTAGTA2200.082.386372
AGGGTAG900.080.555563
ATAGAGC2300.075.652173
CGTAGTG406.660122E-572.52
ACGTAGT406.660122E-572.51
AGAGCCA2500.072.55
GATAGAG2500.069.62
TAGAGCC2750.065.909094
TTGTCTA10350.063.743965
GTAGTAG2850.063.5964933
GTTGTCT10450.062.4401934
CATGAAG954.5474735E-1061.0526351
TAGAGCA606.800925E-660.4166687
TAGTAGA3150.057.539684
Produced by FastQC (version 0.11.5)