FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_36_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_36_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587471
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAGAAATTAACAT104771.7834071809502088No Hit
ATTATGAACAGCTCTCTTTTATTATGGATGCATTTGGCCACTGCTGTAAT72611.2359759034914064No Hit
CTTTTATTATGGATGCATTTGGCCACTGCTGTAATTTTTTAAGAAACATG70651.2026125544920516No Hit
GTTTACAGTTGCTGAAGTTTGATTGATCTGTTGATAAACACTAATGTTTT56470.961238937751821No Hit
CCTATAAATAGTATCAGTGTCATCATCCTTTTTAAGTTAATAATGAGCCA48790.8305090804482264No Hit
GCTTAACCCTAATTATGAACAGCTCTCTTTTATTATGGATGCATTTGGCC48710.8291473111013139No Hit
CATTTGGCCACTGCTGTAATTTTTTAAGAAACATGTTCTCATCTCAACCA45190.7692294598371664No Hit
CCCCAGAAAGGTGGGCAAGTGGCTGTTATTGTTAGTAGGTTTCACTTAGC40470.6888850683693323No Hit
CCCTAATTATGAACAGCTCTCTTTTATTATGGATGCATTTGGCCACTGCT40450.6885446260326042No Hit
GTTTATTTGGTTTGGATATGGGGACATAGGACTCCCCAGAAAGGTGGGCA40330.6865019720122355No Hit
TGCTTAACCCTAATTATGAACAGCTCTCTTTTATTATGGATGCATTTGGC31010.5278558430969358No Hit
GTTGATAAACACTAATGTTTTCCAGTTGACAATTTCTCCATGTTAATTTC30860.525302525571475No Hit
GTGTCAAAGGGACAGTTTATTTGGTTTGGATATGGGGACATAGGACTCCC30320.516110582479816No Hit
CTTCCAGGCAGGTCTGCCAGCGTTAAAGAAATTAACATGGAGAAATTGTC27680.47117219403170535No Hit
GTCAAAGGGACAGTTTATTTGGTTTGGATATGGGGACATAGGACTCCCCA27580.4694699823480648No Hit
CTCTTTTATTATGGATGCATTTGGCCACTGCTGTAATTTTTTAAGAAACA26840.4568736158891248No Hit
ACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTGTTATTGTTAGTAGGT26520.451426538501475No Hit
CACTGATACTATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAG25320.43099999829778834No Hit
GGATATGGGGACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTGTTATT22660.3857211675129496No Hit
ACTGTAAACTGTGCTTTTAGCTAAGTGAAACCTACTAACAATAACAGCCA21760.3704012623601846No Hit
GTGTCAGAAACTGCAGTCAAGCACAACCTGTCCCCAAATCCCAGCCCCTT21300.362571088615438No Hit
GTATCAGTGTCATCATCCTTTTTAAGTTAATAATGAGCCATCGTGTTTCA21100.35916666524815694No Hit
TTATTAACTTAAAAAGGATGATGACACTGATACTATTTATAGGTCCCTTC19790.33686769219246565No Hit
ATGTGGGACAGAGGCAGGTAGACTCTTCCCTAACAGGCAGCAGCAGGATT19240.32750552793244264No Hit
GTTAGTAGGTTTCACTTAGCTAAAAGCACAGTTTACAGTTGCTGAAGTTT18940.32239889288152096No Hit
GCCTGGAAGGGACCTATAAATAGTATCAGTGTCATCATCCTTTTTAAGTT18800.3200157965244242No Hit
GTTATTGTTAGTAGGTTTCACTTAGCTAAAAGCACAGTTTACAGTTGCTG18250.3106536322644011No Hit
CCAGAAAGGTGGGCAAGTGGCTGTTATTGTTAGTAGGTTTCACTTAGCTA17650.3004403621625578No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA17480.2975466023003689No Hit
AGCACAACCTGTCCCCAAATCCCAGCCCCTTGAAACACGATGGCTCATTA17320.294823063606544No Hit
CACTAATGTTTTCCAGTTGACAATTTCTCCATGTTAATTTCTTTAACGCT17290.2943124001014518No Hit
GTTCTCATCTCAACCAAGTTCCAAATGCTAACGTATCAGTGTCAGAAACT17270.29397195776472373No Hit
GGATTATGGCCACAGTGTCAAAGGGACAGTTTATTTGGTTTGGATATGGG16630.28307780298942414No Hit
CTTCCCTAACAGGCAGCAGCAGGATTATGGCCACAGTGTCAAAGGGACAG16350.2783116102752306No Hit
GTTTATCAACAGATCAATCAAACTTCAGCAACTGTAAACTGTGCTTTTAG15900.27065165769884814No Hit
ATTATGGCCACAGTGTCAAAGGGACAGTTTATTTGGTTTGGATATGGGGA15690.2670770131632029No Hit
CTGTTATTGTTAGTAGGTTTCACTTAGCTAAAAGCACAGTTTACAGTTGC15470.26333214745919375No Hit
GCTCTCTTTTATTATGGATGCATTTGGCCACTGCTGTAATTTTTTAAGAA14920.2539699831991707No Hit
GCTAAAAGCACAGTTTACAGTTGCTGAAGTTTGATTGATCTGTTGATAAA14460.24613980945442415No Hit
CTTAAAAAGGATGATGACACTGATACTATTTATAGGTCCCTTCCAGGCAG14160.24103317440350244No Hit
GCGTTAAAGAAATTAACATGGAGAAATTGTCAACTGGAAAACATTAGTGT13700.23320300065875593No Hit
ACATTAGTGTTTATCAACAGATCAATCAAACTTCAGCAACTGTAAACTGT13320.2267345962609218No Hit
CTTTAACGCTGGCAGACCTGCCTGGAAGGGACCTATAAATAGTATCAGTG12870.2190746436845393No Hit
GGTAGACTCTTCCCTAACAGGCAGCAGCAGGATTATGGCCACAGTGTCAA12660.21549999914889417No Hit
GGACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTGTTATTGTTAGTAG12180.20732938306741952No Hit
CTGCTGTAATTTTTTAAGAAACATGTTCTCATCTCAACCAAGTTCCAAAT12150.2068187195623273No Hit
AATTATGAACAGCTCTCTTTTATTATGGATGCATTTGGCCACTGCTGTAA12060.2052867290470508No Hit
GGACAGAGGCAGGTAGACTCTTCCCTAACAGGCAGCAGCAGGATTATGGC12020.20460584437359464No Hit
TCCCAGCCCCTTGAAACACGATGGCTCATTATTAACTTAAAAAGGATGAT11730.19966943049103703No Hit
GCTGTAATTTTTTAAGAAACATGTTCTCATCTCAACCAAGTTCCAAATGC11400.1940521319350232No Hit
ACACTAATGTTTTCCAGTTGACAATTTCTCCATGTTAATTTCTTTAACGC11090.18877527571573743No Hit
CTCCTGGATGTGGGACAGAGGCAGGTAGACTCTTCCCTAACAGGCAGCAG11010.187413506368825No Hit
CCATTTGACTCCTGGATGTGGGACAGAGGCAGGTAGACTCTTCCCTAACA10930.18605173702191258No Hit
TTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAGAAATTAACATGG10640.18111532313935497No Hit
TTCTGACACTGATACGTTAGCATTTGGAACTTGGTTGAGATGAGAACATG10620.18077488080262685No Hit
GAGGCAGGTAGACTCTTCCCTAACAGGCAGCAGCAGGATTATGGCCACAG10410.1772002362669817No Hit
CAGGTAGACTCTTCCCTAACAGGCAGCAGCAGGATTATGGCCACAGTGTC10040.17090205303751163No Hit
GCTCATTATTAACTTAAAAAGGATGATGACACTGATACTATTTATAGGTC9960.1695402836905992No Hit
CTATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAGAAATTAAC9940.1691998413538711No Hit
CATCTCAACCAAGTTCCAAATGCTAACGTATCAGTGTCAGAAACTGCAGT9930.16902962018550702No Hit
GATAAACACTAATGTTTTCCAGTTGACAATTTCTCCATGTTAATTTCTTT9890.16834873551205082No Hit
ATGCTAACGTATCAGTGTCAGAAACTGCAGTCAAGCACAACCTGTCCCCA9820.16715718733350243No Hit
ATAATGAGCCATCGTGTTTCAAGGGGCTGGGATTTGGGGACAGGTTGTGC9810.16698696616513836No Hit
GTCAAGCACAACCTGTCCCCAAATCCCAGCCCCTTGAAACACGATGGCTC9750.16596563915495405No Hit
GTCATCATCCTTTTTAAGTTAATAATGAGCCATCGTGTTTCAAGGGGCTG9650.16426342747131348No Hit
GGTTTGGATATGGGGACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTG9580.1630718792927651No Hit
TGATGACACTGATACTATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCG9480.16136966760912455No Hit
CTGATACTATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAGAA9470.16119944644076048No Hit
GTCAGAAACTGCAGTCAAGCACAACCTGTCCCCAAATCCCAGCCCCTTGA9450.16085900410403237No Hit
CCCTAACAGGCAGCAGCAGGATTATGGCCACAGTGTCAAAGGGACAGTTT9380.15966745592548398No Hit
CATTAGTGTTTATCAACAGATCAATCAAACTTCAGCAACTGTAAACTGTG9120.15524170554801855No Hit
CAGTTTACAGTTGCTGAAGTTTGATTGATCTGTTGATAAACACTAATGTT8960.15251816685419364No Hit
AACATGTTCTCATCTCAACCAAGTTCCAAATGCTAACGTATCAGTGTCAG8820.15013507049709687No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT8690.14792219530836415No Hit
AGTTAATAATGAGCCATCGTGTTTCAAGGGGCTGGGATTTGGGGACAGGT8640.14707108946654388No Hit
GATATGGGGACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTGTTATTG8570.1458795412879955No Hit
CTGTAATGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTC8560.14570932011963145No Hit
GTATCAGTGTCAGAAACTGCAGTCAAGCACAACCTGTCCCCAAATCCCAG8450.14383688726762683No Hit
CCCCAAATCCCAGCCCCTTGAAACACGATGGCTCATTATTAACTTAAAAA8350.14213467558398626No Hit
CATTATTAGTGATATGTACGACTCTAAGACTAAAAATGTTACAAAAGAAA8130.13838980987997707No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC8120.138219588711613No Hit
GTTCCAAATGCTAACGTATCAGTGTCAGAAACTGCAGTCAAGCACAACCT8110.13804936754324895No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA8100.13787914637488488No Hit
TAGTATCAGTGTCATCATCCTTTTTAAGTTAATAATGAGCCATCGTGTTT8080.13753870403815677No Hit
CCTGGATGTGGGACAGAGGCAGGTAGACTCTTCCCTAACAGGCAGCAGCA8040.13685781936470057No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC8000.13617693469124434No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA7960.1354960500177881No Hit
TGACACCACCATCAATTTCCATTTGACTCCTGGATGTGGGACAGAGGCAG7850.1336236171657835No Hit
GTGTCATCATCCTTTTTAAGTTAATAATGAGCCATCGTGTTTCAAGGGGC7810.13294273249232727No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA7800.13277251132396323No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA7790.13260229015559918No Hit
GGTTGTGCTTGACTGCAGTTTCTGACACTGATACGTTAGCATTTGGAACT7630.12987875146177427No Hit
CAGCAGGATTATGGCCACAGTGTCAAAGGGACAGTTTATTTGGTTTGGAT7470.1271552127679494No Hit
GGCAGGTAGACTCTTCCCTAACAGGCAGCAGCAGGATTATGGCCACAGTG7430.1264743280944932No Hit
GATACTATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAGAAAT7380.1256232222526729No Hit
CCTTGAAACACGATGGCTCATTATTAACTTAAAAAGGATGATGACACTGA7360.12528277991594478No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC7350.12511255874758073No Hit
GTTTCACTTAGCTAAAAGCACAGTTTACAGTTGCTGAAGTTTGATTGATC7270.12375078940066828No Hit
GTTTGGATATGGGGACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTGT7000.11915481785483878No Hit
ATCAATTTCCATTTGACTCCTGGATGTGGGACAGAGGCAGGTAGACTCTT6980.1188143755181107No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT6970.11864415434974664No Hit
TCTCAACCAAGTTCCAAATGCTAACGTATCAGTGTCAGAAACTGCAGTCA6900.11745260617119825No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA6850.11660150032937797No Hit
TTTCTGGGGAGTCCTATGTCCCCATATCCAAACCAAATAAACTGTCCCTT6790.11558017331919364No Hit
GTTTCAAGGGGCTGGGATTTGGGGACAGGTTGTGCTTGACTGCAGTTTCT6750.1148992886457374No Hit
GTGTTTATCAACAGATCAATCAAACTTCAGCAACTGTAAACTGTGCTTTT6710.1142184039722812No Hit
GATTATGGCCACAGTGTCAAAGGGACAGTTTATTTGGTTTGGATATGGGG6560.11166508644682034No Hit
GGGCTGGGATTTGGGGACAGGTTGTGCTTGACTGCAGTTTCTGACACTGA6530.1111544229417282No Hit
ATATTAAAGAGGCTTAAATACCATGAAGAAAAAATACACATGGCAAGCAA6360.10826066307953926No Hit
CTGTAAACTGTGCTTTTAGCTAAGTGAAACCTACTAACAATAACAGCCAC6340.10792022074281114No Hit
GTTAATAATGAGCCATCGTGTTTCAAGGGGCTGGGATTTGGGGACAGGTT6340.10792022074281114No Hit
GAAATTAACATGGAGAAATTGTCAACTGGAAAACATTAGTGTTTATCAAC6320.10757977840608303No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC6230.10604778789080653No Hit
GGCCACTGCTGTAATTTTTTAAGAAACATGTTCTCATCTCAACCAAGTTC6230.10604778789080653No Hit
GTTAATTTCTTTAACGCTGGCAGACCTGCCTGGAAGGGACCTATAAATAG6180.10519668204898626No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA6170.1050264608806222No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG6170.1050264608806222No Hit
CCACCATCAATTTCCATTTGACTCCTGGATGTGGGACAGAGGCAGGTAGA6160.10485623971225815No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC6130.10434557620716597No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC6120.1041753550388019No Hit
CCATCAATTTCCATTTGACTCCTGGATGTGGGACAGAGGCAGGTAGACTC6110.10400513387043785No Hit
GGATGATGACACTGATACTATTTATAGGTCCCTTCCAGGCAGGTCTGCCA6100.10383491270207379No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA5990.1019624798500692No Hit
GGCTGTTATTGTTAGTAGGTTTCACTTAGCTAAAAGCACAGTTTACAGTT5970.10162203751334109No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTC100.007090124144.999988
GTAAGTA100.007090124144.999984
GACATGC650.0111.538461
TGCTTAA5600.095.803571
GACCGAG406.1143146E-790.624997
GGACCGA406.1143146E-790.624996
TCACGCA850.085.294112
ATCACGC850.085.294111
ACGCACT801.8189894E-1281.562494
GCTTAAC12600.081.130951
CTTAACC13050.078.3333362
AACCCTA13250.077.698115
TTAACCC13350.076.573043
ATAGGCG300.001935343572.59
CACGCAC1000.072.53
ACCCTAA14700.069.540826
TAATGTA1600.067.968744
ATATGCC8700.067.500013
TATATGC8600.066.598832
TTGGCCG1150.063.043483
Produced by FastQC (version 0.11.5)