FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_35_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_35_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594709
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTT94561.5900213381670698No Hit
GTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC69811.1738514130440267No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCC61141.0280658271524392No Hit
GGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAA59260.9964537277895575No Hit
GGTTCAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA54200.911370098653291No Hit
CCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTCACCAATA51520.8663060421147148No Hit
CCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG46510.7820631603019292No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATAT40050.6734386061081975No Hit
CACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA35050.5893638737601079No Hit
CATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAG33070.5560702797502645No Hit
CCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACC31430.528493767540091No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAA29210.49116458637753924No Hit
GTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCAT28820.4846067572543883No Hit
GCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTT25050.42121440906392876No Hit
CAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCAC24120.4055765088471841No Hit
CCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC23600.39683273668298275No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG22290.3748051568077833No Hit
CAATACCAATGTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTG20790.34958273710335647No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT20710.348237541385787No Hit
CTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGC20450.34386565530368635No Hit
GTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCA20010.33646707885705446No Hit
CCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGAC19990.3361307799276621No Hit
GTTCACCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTCAC17810.299474196623895No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT17210.2893852287421243No Hit
GGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATA17050.2866948373069854No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATATTGGTG16950.28501334266002365No Hit
CCAATGTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTGGGTGT16400.2757651221017338No Hit
ATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCAC16100.2707206381608484No Hit
CCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAACT16030.26954359190797517No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT15270.25676423259106557No Hit
GTTCAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAG14850.24970195507382603No Hit
GCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCC14240.23944483772735908No Hit
ATACCAATGTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTGGG13760.23137366342194252No Hit
CAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGTTG13630.2291877203808922No Hit
GTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAACTG13370.2248158342987915No Hit
ATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCA13370.2248158342987915No Hit
GTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA13170.22145284500486792No Hit
AACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCG12500.21018683087022394No Hit
CACAATACCAATGTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTT11680.19639857476513722No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG11590.1948852295828716No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC11560.1943807811887831No Hit
GGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACA11500.19337188440060601No Hit
CAATTACCAATGTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTTT10960.18429181330701233No Hit
ATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTC10890.1831147670541391No Hit
ATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGT10860.18261031866005054No Hit
TCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCG10820.18193772080126583No Hit
GAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTG10620.17857473150734224No Hit
GGTATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACAAA10540.1772295357897728No Hit
GGTGAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATATTGGTGAA10400.17487544328402632No Hit
CAATACCAATGTTCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTG10380.17453914435463394No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGGTGGGCCTCATAGCACA10260.1725213507782798No Hit
CTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGC10190.17134430452540655No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC10100.16983095934314094No Hit
GGTTCACAGATGCGCAAACAGGTTCACTGTTCCTGGAATATTGGTGAACA10030.1686539130902677No Hit
TTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAA9880.166131671119825No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG9620.16175978503772434No Hit
CCCAAAAATGGATCATTACAAAATTGGTGGGCCTCATAGAACATTGGTAT9360.1573878989556237No Hit
CATCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTG9360.1573878989556237No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA9340.1570516000262313No Hit
TTACAAAATTGGTGGGCCTCATAGAACATTGGTATTGTGGGCTCAATGTG9200.1546975075204848No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA9180.15436120859109245No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAAC9100.153016012873523No Hit
CACCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCG9100.153016012873523No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGT8970.15083006983247269No Hit
AACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCAA8950.15049377090308033No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG8940.15032562143838415No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT8700.14629003428567586No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA8680.1459537353562835No Hit
GGGTAATAATGACTTGTTGGTGATTGTAGATATTGGGCTGTTAATTGTCA8680.1459537353562835No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT8490.1427588955270561No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT8270.13905960730374015No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT8270.13905960730374015No Hit
TGATAGAGCCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTG8090.13603291693920894No Hit
CCCAAAAATGGATCATTACAAAATTGGTGGGCCTCATAGCACATTGGTAT7940.13351067496876623No Hit
CACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTG7860.1321654792511968No Hit
ATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTG7850.1319973297865006No Hit
ACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCAC7790.13098843299832355No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTTACTCTCTCTACAAGGTT7660.12880248995727323No Hit
CTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGT7640.12846619102788087No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAG7630.1282980415631847No Hit
ACTATGGGTGTTAAATTTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG7600.12779359316909614No Hit
TGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACA7510.12628024798683055No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC7350.12358985655169168No Hit
GGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGTTGG7310.12291725869290696No Hit
TCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAAT7290.1225809597635146No Hit
CAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTG7280.12241281029881841No Hit
ATACCAATGTTCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTGGG7220.12140391351064134No Hit
CAATACAGGTTCATCGGTGCCTGGAATATTGGTGAACAGATGCGCAAACA7210.12123576404594516No Hit
GGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTC7040.11837722314611011No Hit
GGCACCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC7020.11804092421671775No Hit
TTGGTATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA7020.11804092421671775No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA7010.11787277475202157No Hit
TCTGTTCACCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTT6980.11736832635793305No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC6850.11518238331688271No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC6730.11316458974052857No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA6700.11266014134644002No Hit
GTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATAT6640.11165124455826295No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGT6560.11030604884069352No Hit
TACTATGGGTGTTAAATTTTTTTACTCTCTCTACAAGGTTTTTTCCTAGT6530.10980160044660497No Hit
GATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAA6460.10862455419373172No Hit
ATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGC6440.10828825526433936No Hit
AGCCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTG6380.10727935847616228No Hit
ATGTTCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTGGGTGTCTG6330.10643861115268138No Hit
GAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATATTGGTGAACAG6280.10559786382920049No Hit
CTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC6270.10542971436450432No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG6240.10492526597041579No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC6180.1039163691822387No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC6160.10358007025284635No Hit
TTACAAAATTGGTGGGCCTCATAGCACATTGGTATTGTGGGCTCAATGTG6160.10358007025284635No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA6140.103243771323454No Hit
CTGTTCACCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTC6090.1024030239999731No Hit
CAGGTTCAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTT6070.10206672507058073No Hit
ATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA6050.10173042614118838No Hit
GTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTGGGTGTATTGT6010.10105782828240366No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGT6000.10088967881770748No Hit
ACCTTGTAGAGAGAGTAAAAAAATTTAACACCCATAGTAGGCCTAAAAGC6000.10088967881770748No Hit
CACAATACCAATGTTCTATGAGGCCCACCAATTTTGTAATGATCCATTTT5990.1007215293530113No Hit
ATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA5980.10055337988831513No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT5970.10038523042361894No Hit
GAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCAATGTG5950.10004893149422658No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA100.0070901453144.99998145
GGGTAGT650.0111.538461
TGATGTA502.3050889E-672.51
GGTAGTA1100.065.9090962
GTAGTAG1009.094947E-1265.253
AGTAGAG1051.6370905E-1162.1428575
GATGTAG606.8022146E-660.4166682
GGTCTGC851.3042154E-859.7058831
TTGTCTA4800.054.3755
CGACGGG553.217939E-452.727276145
TACGTAG701.6951468E-551.7857135
GCATTAT858.9186324E-751.176471
CCCTAGC13400.050.8582081
CCTAGCA14050.048.5053372
TAGCATT14050.048.5053374
ACAGTGT1056.9001544E-848.3333328
GGTCGGG450.00963722948.333332145
CATGAAG1501.0913936E-1148.3333321
ACGTAGT752.549326E-548.3333326
CGCCGTG604.9434544E-448.333332145
Produced by FastQC (version 0.11.5)