FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_35_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_35_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695502
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGT104441.501649168514253No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAA79041.1364453301356432No Hit
TCCCTATGGTGCTACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATG65670.9442100813513117No Hit
TCCCTATGGTGCTAACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGG60910.8757703069150052No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGTAGCACCATAGGG53490.769084776176057No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACC53170.7644837829366414No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTTAGCACCATAGGGA43530.6258788615992477No Hit
TCCCTATGGTGCTACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATG41570.5976977780078274No Hit
GTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGT38640.5555699336594287No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAAAGCACTCGTGGACA37480.5388913331665474No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGTAGCA36790.5289704414940575No Hit
GTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCAGCTGT35960.5170366152793234No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGGGTAGCACCATAGGG33980.4885679696104397No Hit
GTCCAGCTGTCCACGAGTGCTTTGCAGTAGCACCATAGGGAAGTCCAGCT30610.44011375955784454No Hit
CAGCTGGACTTCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTGGACT29940.4304804299628182No Hit
TCCCTATGGTGCTACAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTAT29610.425735655684671No Hit
GTCCACGAGTGCTTTGCAGTAGCACCATAGGGAAGTCCAGCTGTCCACGA28530.41020730350164347No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTTAGCAC28230.4058938723396913No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTGTAGCACCATAGG27370.39352870300876197No Hit
GTGCTACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACC27020.3884963666531513No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGGGTAGCA24450.35154463969909505No Hit
GTGCTACAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAC24300.34938792411811903No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTACTGCAAAGCACTCGTGGA24090.34636852230475257No Hit
GTGCTACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACC23670.34032971867801964No Hit
GTCCACGAGTGCTTTGGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAG22640.32552027168865083No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTAACCAAAGCACTCGTGGAC22160.31861878182952746No Hit
GTCCAGCTGTCCACGAGTGCTTTGGTTAGCACCATAGGGAAGTCCAGCTG20440.2938884431676688No Hit
GTCCAGCTGTCCACGAGTGCTTTGGGGTAGCACCATAGGGAAGTCCAGCT19800.2846864566888377No Hit
GTCCACGAGTGCTTTGGGGTAGCACCATAGGGAAGTCCAGCTGTCCACGA19360.2783600909846413No Hit
CAGCTGGACTTCCCTATGGTGCTACTGCAAAGCACTCGTGGACAGCTGGA19160.2754844702100066No Hit
TCCCTATGGTGCTACCAGCAAAGCACTCGTGGACAGCTGGACTTCCCTAT19110.2747655650163479No Hit
GCTGGACTTCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTGGACTTC18840.270883476970591No Hit
TCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTA18400.26455711126639464No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTGTAGC18320.26340686295654075No Hit
ACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAAAGC17880.2570804972523444No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTACCCCAAAGCACTCGTGGA16690.23997055364326775No Hit
CAGCTGGACTTCCCTATGGTGCTAACCAAAGCACTCGTGGACAGCTGGAC16660.23953921052707253No Hit
GTCCAGCTGTCCACGAGTGCTTTGGCTGTAGCACCATAGGGAAGTCCAGC16130.2319188154742905No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCTGGTAGCACCATAGG16070.23105612924190008No Hit
GTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAA15620.22458598249897196No Hit
ACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAAAGC15240.21912230302716598No Hit
GTCCACGAGTGCTTTGGCTGTAGCACCATAGGGAAGTCCAGCTGTCCACG14870.21380240459409175No Hit
GTCCAGCTAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCAG14700.2113581269356522No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGT14430.20747603888989535No Hit
GGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACCA14230.20460041811526064No Hit
TTGCAGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGC14170.2037377318828702No Hit
CCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGTAGCACCATAG14050.20201235941808937No Hit
ATGGTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAC13350.19194768670686785No Hit
GCTAGCTGGACTTCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTGGA13220.19007853320335527No Hit
GTGCTAACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCA12920.1857651020414032No Hit
CAGCTGGACTTCCCTATGGTGCTACCCCAAAGCACTCGTGGACAGCTGGA12780.18375216749915887No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTACAGCCAAAGCACTCGTGG12640.18173923295691458No Hit
TTGGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCA12450.17900739322101158No Hit
GCTGGACTTCCCTATGGTGCTACTGCAAAGCACTCGTGGACAGCTGGACT12300.17685067764003554No Hit
GCTGGACTTCCCTATGGTGCTAACCAAAGCACTCGTGGACAGCTGGACTT11840.17023674985837567No Hit
ACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACTGCAAA11790.16951784466471698No Hit
TCCCTATGGTGCTACTGCAAAGCACTCGTGGACAGCTAGCTGGACTTCCC11730.1686551584323266No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTGACCATAGGGAAGT11730.1686551584323266No Hit
GCTTTGCTGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGG11520.1656357566189601No Hit
TCCCTATGGTGCTAACCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCT11140.16017207714715415No Hit
GTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACTGC11060.15902182883730026No Hit
GCTTTGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAG10880.156433770140129No Hit
GCTTTGGCTGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGG10650.15312680624929909No Hit
GCTTTGCAGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGT10480.15068252859085954No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCTGGTAGC10390.14938849924227393No Hit
GTGCTACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACT10250.14737556470002963No Hit
ACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAACCAAAG10250.14737556470002963No Hit
CTTGTGATGGTGGTGTCACTCGTGGACAGCTGGACTTCCCTATGGTGCTA10140.14579397327398053No Hit
GGTGACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATA10060.14464372496412664No Hit
CCATAGGGAAGTCCAGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGT10000.1437810387317362No Hit
GTGCTACCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATGGTGCTA9920.14263079042188231No Hit
GTGCTACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAAC9780.14061785587963802No Hit
ATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAAGT9760.14033029380217454No Hit
ACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACTGCAAA9720.1397551696472476No Hit
CCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGCAGTAGCACCAT9520.13687954887261286No Hit
CAGCTGGACTTCCCTATGGTGCTACAGCCAAAGCACTCGTGGACAGCTGG9480.13630442471768595No Hit
GTCCAGCTGTCCACGAGTGCTTTGCTGGTAGCACCATAGGGAAGTCCAGC9400.13515417640783203No Hit
GGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGTAGCAC9290.13357258498178295No Hit
GGGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCAC9290.13357258498178295No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCTAGCTGT9110.13098452628461169No Hit
GTGCTACCCCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATGGTGC9000.1294029348585626No Hit
GTGCTACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACT8920.1282526865487087No Hit
TCCCTATGGTGCTACCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGG8480.12192632084451231No Hit
GTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAACCA8380.12048851045719496No Hit
GCTTTGGGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGT8300.11933826214734104No Hit
ACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAACCAAAG8260.11876313799241411No Hit
GCTAGCTGGACTTCCCTATGGTGCTACTGCAAAGCACTCGTGGACAGCTG8260.11876313799241411No Hit
ACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAAAGCAC8260.11876313799241411No Hit
GTGCTACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAAC8230.1183317948762189No Hit
GTCCAGCTAGCTGTCCACGAGTGCTTTGCAGTAGCACCATAGGGAAGTCC8170.11746910864382848No Hit
CTACAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAA8170.11746910864382848No Hit
GGTTAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGTAG8120.11675020345016981No Hit
GTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCAGCTAG7990.11488104994665724No Hit
GCTGGACTTCCCTATGGTGCTACCCCAAAGCACTCGTGGACAGCTGGACT7960.11444970683046202No Hit
CCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGTTAGCACCATA7950.11430592579173028No Hit
TTGCAGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGTA7900.11358702059807162No Hit
GGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACC7800.11214921021075425No Hit
GCTAGCTGGACTTCCCTATGGTGCTAACCAAAGCACTCGTGGACAGCTGG7780.11186164813329078No Hit
TTGGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGTAG7760.11157408605582729No Hit
GTGCTACTGCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATGGTGC7730.1111427429396321No Hit
CCCTATGGTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTG7720.11099896190090036No Hit
GGTGACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGTAGCACCA7620.10956115151358299No Hit
GTCCACGAGTGCTTTGCTGGTAGCACCATAGGGAAGTCCAGCTGTCCACG7610.10941737047485126No Hit
TTGCAGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTTAG7610.10941737047485126No Hit
ACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCCCAAA7580.10898602735865605No Hit
GCTTTGGTAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTG7480.10754821697133868No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGGTAGCACCATAGGGA7340.10553528242909438No Hit
TCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTAGCAAAGCACTCGTG7300.10496015827416744No Hit
GGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCA7270.10452881515797222No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAGCAAAGCACTCGTGG7260.1043850341192405No Hit
GCTACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAA7240.10409747204177702No Hit
GTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCAGCTAGCTGTCCAC7220.10380990996431355No Hit
GTCCAGCTAGCTGTCCACGAGTGCTTTGGTTAGCACCATAGGGAAGTCCA7210.1036661289255818No Hit
GTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCCC7170.10309100477065487No Hit
GTGCTAACCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATGGTGCT7150.1028034426931914No Hit
GTGCTAACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACTG7000.10064672711221534No Hit
GGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTTAGCACC6970.10021538399602015No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTATG100.0070904023144.99998145
CTTGATG2200.072.500011
TATATGC502.3055018E-672.52
TATGCCT502.3055018E-672.54
ATATGCC554.0603045E-665.9090963
TTATATG554.0603045E-665.9090961
TACTAAT350.00356604762.1428572
TGACCTT400.006050115554.374996145
GAGAAAA400.006050115554.374996145
GGCGTGC604.9440365E-448.333332145
ACTCACG657.335035E-444.615387145
GCTTTAG657.335035E-444.6153871
GTGGCGG700.00105641941.42857145
TGATGGG800.0020361936.249996145
ACCACGG850.002741413434.11765145
AGAGAGG2601.10958354E-1030.673079145
GGTTAGC154300.030.6351261
GGTACGA1703.0099764E-629.8529422
TGTGTTG2701.72804E-1029.5370373
GGTTGAT1000.006073203429.07
Produced by FastQC (version 0.11.5)