FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_33_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_33_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473275
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTAC29750.6285985948972584No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA22930.48449632877291215No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT22890.4836511541915377No Hit
GTCTTATTACAGTAGGCTAAGATAAGTGCACAAAAGTTAGCAGCTTCACC22160.4682267180814537No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT19170.40504991812371244No Hit
GTAATAAGCATCTTGTAGAGCAGGTGGATTAAACTTCAACTCTATTTGTT18160.3837092599440072No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC18060.38159632349057104No Hit
GTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCA16880.3566636733400243No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG16640.35159262585177753No Hit
GGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTTAAGT15710.33194231683482117No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG15670.3310971422534467No Hit
TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT15610.32982938038138504No Hit
GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG15210.3213776345676404No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA14380.30384026200412023No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC14240.3008821509693096No Hit
CCCTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAA14180.2996143890972479No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT13900.29369816702762663No Hit
GCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTAT13740.29031746870212877No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT13290.28080925466166606No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG13200.27890761185357354No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC11950.25249590618562145No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG11030.23305689081400877No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG10840.22904231155248003No Hit
CTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTT10650.22502773229095135No Hit
GTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTA10590.22375997041888968No Hit
CATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTA10460.22101315302942262No Hit
AGTTCATACTGAGCAGGTGGTGCTGACATCATAACAAAAGGTGACTCCTG10430.2203792720933918No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT10330.21826633563995562No Hit
CCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAA10140.21425175637842692No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT9800.20706777243674396No Hit
GTAGATAAAAGTGCATTAACATTGGCCGTGACAGCTTGACAAATGTTAAA9380.1981934393323121No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT9150.1933336854894089No Hit
GGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTT8950.1891078125825366No Hit
CTCCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAG8870.18741746341978766No Hit
CTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTT8860.18720616977444404No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC8830.18657228883841318No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC8560.18086736041413554No Hit
CTATTAGCATAAGCAGTTGTGGCATCTCCTGATGAGGTTCCACCTGGTTT8280.17495113834451428No Hit
GTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATAAAGAAC8210.17347208282710896No Hit
GTTTTACACACCACGTTCAAGACTCTTTTGCAAGAATCTAAATTGGCATG8070.17051397179229835No Hit
GCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAAC8050.17009138450161113No Hit
AGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTG7970.1684010353388622No Hit
GATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGA7820.16523163065870794No Hit
CTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAAGA7750.16375257514130265No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT7690.16248481326924094No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC7660.16185093233321007No Hit
GAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGC7630.16121705139717923No Hit
ACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAA7550.1595267022344303No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT7550.1595267022344303No Hit
GTTTATAATCAATAGCCACCACATCACCATTTAAGTCAGGGAAAAATGTA7440.1572024721356505No Hit
GTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGT7430.1569911784903069No Hit
GTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTA7380.15593471026358882No Hit
TTCTTAAATTGTTCATAAGAAAGTGTGCCCATGTACATAACAGCTTCTAC7320.15466694839152711No Hit
ATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTA7210.15234271829274734No Hit
ATTTATAAGTAACTGGTTTTATGGTTGTTGTGTAACTGTTTTCTTTGTAG7160.15128625006602925No Hit
CCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATC7120.1504410754846548No Hit
ATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTAT7110.15022978183931118No Hit
ACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGA7000.1479055517405314No Hit
ATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAA6970.14727167080450057No Hit
CATAAAAACATTGTTTTGATAATAAAGAACTGACTTAAAGTTCTTTATGC6820.14410226612434632No Hit
CACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGT6810.1438909724790027No Hit
CATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGA6790.14346838518831548No Hit
CGTACAAGGTATCTGAACACCTTTCTTAAATTGTTCATAAGAAAGTGTGC6760.1428345042522846No Hit
GAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTG6760.1428345042522846No Hit
GGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGC6670.14093286144419206No Hit
GAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCA6530.13797475040938145No Hit
GTAAATGTACCATGCTTAAGTTCATACTGAGCAGGTGGTGCTGACATCAT6530.13797475040938145No Hit
CATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAG6480.13691828218266336No Hit
ATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGA6430.13586181395594527No Hit
TTTGTATTCTGAGGACTTTGTAAGTAAAGCACCGTCTATGCAATACAAAG6410.13543922666525804No Hit
GTCCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAA6380.1348053457292272No Hit
ATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGAC6340.13396017114785272No Hit
GCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGAGTATCATCAT6290.13290370292113463No Hit
GCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTC6270.1324811156304474No Hit
CCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAG6270.1324811156304474No Hit
GTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAG6260.13226982198510379No Hit
GTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCACA6250.13205852833976017No Hit
GTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTG6220.13142464740372933No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC6170.13036817917701124No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA6130.1295230045956368No Hit
CCCTAGCATTGTTAACATGCCAAACAATAGGTTTATGTAACAATTTAGCT6040.12762136178754424No Hit
CTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGT6030.12741006814220063No Hit
GTACTAGATATTTTGTAGCTTGTTTACCACACGTACAAGGTATCTGAACA6030.12741006814220063No Hit
GTTTTATGGTTGTTGTGTAACTGTTTTCTTTGTAGAAAACATCCGTAATA6030.12741006814220063No Hit
CTTATAACCAGTTAACTGGTTTAAATCATCAGCAAATTTGATATTATCAC5990.12656489356082615No Hit
GAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGC5920.12508583804342085No Hit
TTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACAC5900.1246632507527336No Hit
GTTCAAGACTCTTTTGCAAGAATCTAAATTGGCATGTTGAAACAAGTAAC5880.12424066346204637No Hit
CAACAGCATCGTCAGAGAGTATCATCATTGAGAAATGTTTACGCAAATAT5860.12381807617135915No Hit
CTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAA5840.12339548888067192No Hit
GTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGT5810.12276160794464105No Hit
CTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACA5810.12276160794464105No Hit
GCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTT5750.12149384607257938No Hit
CACTAGCACAAGTAAATGTACCATGCTTAAGTTCATACTGAGCAGGTGGT5620.11874702868311235No Hit
GTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTT5570.11769056045639427No Hit
GTCAGAGAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACT5550.11726797316570704No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC5530.11684538587501982No Hit
GAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACAC5500.11621150493898895No Hit
ATCACATACAAACTTAAAATTATCGAAGCTTGCGTTTGGATATGGTTGGT5440.11494374306692727No Hit
CTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATC5400.11409856848555279No Hit
CCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTC5310.11219692567746024No Hit
GTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCAC5310.11219692567746024No Hit
TCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACG5270.11135175109608579No Hit
AATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGA5230.11050657651471131No Hit
CATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGT5210.11008398922402408No Hit
ACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGA5180.10945010828799324No Hit
GTCTGTGTCAACATCTCTATTTCTATAGAGACACTCATAAAGTCTGTGTT5120.10818234641593154No Hit
CTCTATTTCTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGACA5110.10797105277058792No Hit
CAATAGGTTTATGTAACAATTTAGCTCCTTTCTTAAAAGAGGGTGTGTAG5090.1075484654799007No Hit
GTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATA5050.10670329089852623No Hit
GTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAG5050.10670329089852623No Hit
GTTATATGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGC4980.10522423538112091No Hit
AGATAAAAGTGCATTAACATTGGCCGTGACAGCTTGACAAATGTTAAAAA4980.10522423538112091No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA4960.10480164809043369No Hit
TATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAG4940.10437906079974645No Hit
GTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATC4920.1039564735090592No Hit
GTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA4890.10332259257302837No Hit
CCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATT4870.10290000528234114No Hit
GTGCTATTGAAACACACAACAGCATCGTCAGAGAGTATCATCATTGAGAA4840.10226612434631027No Hit
ATGCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGAGTATCATC4830.10205483070096666No Hit
TAATAGGACCTTTGTATTCTGAGGACTTTGTAAGTAAAGCACCGTCTATG4830.10205483070096666No Hit
CTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGAC4810.10163224341027943No Hit
AGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGT4770.10078706882890498No Hit
AGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGG4750.10036448153821773No Hit
ATTGAAACACACAACAGCATCGTCAGAGAGTATCATCATTGAGAAATGTT4740.10015318789287411No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAGGC100.00708969145.03
AGTGCGC100.00708969145.08
GAAGGGT100.00708969145.01
GGAGGCT100.00708969145.04
TAGAGCC2750.092.272734
ATTATGC651.6370905E-1189.230773
ATAGAGC3000.084.5833363
GAGCCAT3350.075.746276
TCGCCGG502.3043613E-672.5145
AGAGCCA3550.071.4788745
GATAGAG3800.066.776322
ATAGTAC554.058302E-665.909097
GTGATAG2000.065.251
TGGGTGT2450.062.1428576
AATATCG350.00356534362.1428575
AGGGGGG350.00356534362.142857145
GCGTAAT350.00356534362.1428571
TTGGGTG2450.062.1428575
CCATGCC4100.061.8902449
TAGATAG606.8000754E-660.4166684
Produced by FastQC (version 0.11.5)