FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_33_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_33_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624357
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT24620.3943256822619111No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA20040.32097021415632404No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG19510.31248148094759887No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG18640.2985471453030878No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA17510.2804485254429757No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT13730.21990623953923796No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC13390.21446063710345203No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG12400.19860432412866358No Hit
GGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGC12260.19636201724333993No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC11570.18531064759424495No Hit
GTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTT11380.18226751682130576No Hit
GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT11350.1817870224887364No Hit
CTCTTAAGAAGTTGAATGTCTTCACCTTTGTTAACATTTGGGCCGACAAC11200.1793845508258897No Hit
GTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAA10870.17409911316762686No Hit
ATAATACCCATAGCAAAAGGTAAAAAGGCATTTTCATACAAAAAAAAGAA10750.17217713583734948No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA10570.16929416984193338No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG10550.16897384028688717No Hit
ACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAG10400.16657136862404043No Hit
TTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA10390.1664112038465173No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC10320.1652900504038555No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA10230.16384856740614745No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA10010.1603249423006389No Hit
CATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAA10000.16016477752311578No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA9850.15776230586026904No Hit
CTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGA9770.1564809876400841No Hit
GTCTAAAGTAGCGGTTGAGTAAACAAAAGAGGCCAAAGTAACAAGTACAA9610.15391835119971425No Hit
CTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTT9430.15103538520429818No Hit
CTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCTGATAATAAT9230.14783208965383587No Hit
GTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCAT9220.14767192487631275No Hit
ATTCATAAGTGTCCACACTCTCCTAGCACCATCATCATACACAGTTCTTG9160.14671093621117406No Hit
GGCATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAA9140.14639060665612783No Hit
GCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC8950.14334747588318864No Hit
ACATAACACAGTCTTTTAGCTTAAAACCAGACAAACTAGTATCAACCATA8910.14270681677309616No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA8880.14222632244052683No Hit
GTCTAACAACATCAAAAGGTGTAAATTCATCTTCTAATAAAGCACTACCC8740.13998401555520318No Hit
GTATCTACACAAACTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACC8660.13870269733501828No Hit
GATTATAAGAGCCCACATGGAAATGGCTTGATCTAAAGCATTACCATAAT8660.13870269733501828No Hit
ATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCT8560.1371010495597871No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC8460.13549940178455594No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA8450.13533923700703282No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC8230.1318156119015243No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC8200.13133511756895494No Hit
CTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG8190.13117495279143182No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT8180.1310147880139087No Hit
GTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGA8180.1310147880139087No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG8180.1310147880139087No Hit
CATAAAAACAATACCTCTGGCCAAAAACATGACAGTTGTAACTACACCTG8040.12877248112858508No Hit
ATCTAAAGCATTACCATAATAAACTTTATAAACGAGTGTCAAGACATTCA8010.12829198679601575No Hit
GCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATT7990.1279716572409695No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC7820.12524885602307653No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC7820.12524885602307653No Hit
ATTGAGTACTCTGGACTAAAACTAAAAGTGAAGTCAAAATTGTGAGTAAC7790.1247683616905072No Hit
GTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCT7580.12140490136252176No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT7560.12108457180747553No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT7400.11852193536710567No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT7390.11836177058958255No Hit
ATTTTAGACTGTACAGTGGCTACTTTGATACAAGGTTTGCCACCAACACC7370.11804144103453633No Hit
GTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGAC7360.11788127625701322No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG7350.1177211114794901No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC7320.11724061714692074No Hit
GATAATAATGGTGCAAGTAGAACTTCGTGCTGATTAAAATTTTCATAAGC7300.11692028759187452No Hit
GTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTT7230.11579913414921271No Hit
AACTAGTATCAACCATATCCAACCATGTCATAATACGCATCACCCAACTA7140.11435765115150467No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT7000.11211534426618104No Hit
CAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAAT6790.10875188393819561No Hit
GTTTTTATGTGTGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATAC6780.1085917191606725No Hit
CTTGTAGATCTGTTCTCTAAACGAACATGAAAATTATTCTTTTCTTGGCA6760.10827138960562627No Hit
GTATAGGGTCAGCACCAAAAATACCAGCTGATAATAATGGTGCAAGTAGA6620.10602908272030265No Hit
ATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTATGCATCA6560.10506809405516396No Hit
GGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTG6530.10458759972259461No Hit
ATTTAATGTTGAGTTTGAAGGCATCTATGCTATTCTTGGGTGGGAGTAGT6420.10282578716984032No Hit
ATTCTACTCTGAGTTGTTGCAAAACTGAGAGTAAGACTACTGATGTGCAC6380.10218512805974787No Hit
GTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTTACTCTCAGTTT6350.10170463372717851No Hit
CCATTAAGGAATGAACCCTTAATAGTGAAATTGGGCCTCATAGCACATTG6340.10154446894965541No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC6330.10138430417213229No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT6330.10138430417213229No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG6290.10074364506203982No Hit
GTCATAAGGATTAGTAACACTACAGCTGATGCATACATAACACAGTCTTT6260.10026315072947048No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGC100.0070902277145.000023
CCACCCG100.0070902277145.00002145
TGCGCTA608.2945917E-1084.5833367
TATGCGC651.5625119E-978.076925
ATGCGCT702.8103386E-972.56
GAGCAGG300.001935400872.56
CCTGCTC955.456968E-1268.684218
ATATGCG754.8512447E-967.6666644
TCCAAGG702.3331086E-762.1428572
GGTGTTC805.870115E-754.3750048
GGAGTCA400.006049824454.3750048
AGCCTGC1206.002665E-1154.3756
AAATTAG6500.053.538465
GCGCTAG953.1368472E-853.4210558
AAGGGTG858.91936E-751.176475
TCAAATG1950.048.3333362
GTCTACC1109.951327E-846.1363689
AGGAGTA803.7330305E-545.3125047
GAAGGAG803.7330305E-545.3125045
AATTAGC2150.043.8372082
Produced by FastQC (version 0.11.5)