FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_32_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_32_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456763
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT57161.2514148475248652No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG46231.0121222603407019No Hit
CCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGAC44060.964614033973855No Hit
ATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGT41260.903313096726311No Hit
CCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATT31700.694014182409696No Hit
GTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA28950.6338079047558581No Hit
GGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACG26740.5854239507140465No Hit
CCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAAC25690.5624360992462174No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTG25660.5617793034899937No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT24670.5401050435346121No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC24050.5265312645726559No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA23910.5234662177102787No Hit
CACCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCG21980.4812123573932214No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT21760.4763958551809144No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA21300.46632498691881785No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT21160.46325994005644067No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT19590.42888762881406767No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT19510.42713617346413785No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC18970.4153138498521115No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT18460.4041483219963088No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT17990.393858521815471No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG17010.3724031937788306No Hit
ATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG16630.3640837808666639No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC16310.3570779594669446No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC15300.3349658356740804No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC15060.32971146962429093No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG15050.3294925377055497No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT14550.31854594176848827No Hit
CTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC14190.310664392693804No Hit
ATTTTTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAG13760.3012503201879312No Hit
GTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCA13580.29730954565058904No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC13500.2955580903006592No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC13240.28986586041338724No Hit
ATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA13180.2885522689009399No Hit
TGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC13020.2850493582010802No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC11340.24826879585255374No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT11040.24170083829031688No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT11030.2414819063715756No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC10840.23732219991549228No Hit
GCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTT10690.23403822113437384No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAA10640.2329435615406677No Hit
GTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAA10610.23228676578444402No Hit
CAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCT9550.20907998239787373No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG9380.20535813977927286No Hit
TCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG9370.20513920786053164No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA9290.20338775251060176No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT9270.2029498886731193No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAA9200.2014173652419307No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG9190.20119843332318949No Hit
TTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTA9160.2005416375669658No Hit
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC9110.19944697797325966No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT8660.18959504162990434No Hit
ATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA8250.18061883296151396No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT8120.177772718017878No Hit
GGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCT8050.17624019458668938No Hit
ATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCG8050.17624019458668938No Hit
GTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGA8010.17536446691172447No Hit
GGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGAT7880.17251835196808848No Hit
GATCCATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAG7860.17208048813060603No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGC7760.16989116894319373No Hit
GGGTAAGGAAGGTACACATAATCATCACCCTGTTTAACTAGCATTGTATG6990.1530334112001191No Hit
GATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC6970.15259554736263664No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA6940.15193875160641296No Hit
GTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATT6910.15128195585018928No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC6760.14799797706907084No Hit
ATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTC6570.14383827061298748No Hit
TCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCA6430.14077322375061027No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT6300.13792710880697429No Hit
GTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCT6160.1348620619445971No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT6110.13376740235089096No Hit
GGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAG6110.13376740235089096No Hit
GAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCT6080.13311060659466725No Hit
CATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTT5990.1311402193259962No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG5990.1311402193259962No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG5880.12873196821984267No Hit
CTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTT5860.12829410438236022No Hit
TACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAAC5850.128075172463619No Hit
TTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAA5820.1274183767073953No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG5760.12610478519494792No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCT5700.12479119368250055No Hit
CTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCAC5630.12325867025131194No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC5600.12260187449508826No Hit
AGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGA5590.12238294257634703No Hit
GAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA5530.12106935106389967No Hit
GTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCA5490.12019362338893473No Hit
CTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTT5470.11975575955145228No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA5460.11953682763271105No Hit
ACATAAGAATGGTCTACGTATGCAAGCACCACATCTTAATGAAGTCTGTG5440.1190989637952286No Hit
GTCTAGGTTTACCAACTTCCCATGAAAGATGTAATTCTCTGTCAGACAGC5300.1160339169328514No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC5270.11537712117662771No Hit
TTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCT5240.11472032542040403No Hit
GTAATGATCCATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT5240.11472032542040403No Hit
AGCAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAG5220.11428246158292155No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTA5220.11428246158292155No Hit
AGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTT5190.11362566582669786No Hit
GGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT5190.11362566582669786No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGG5150.11274993815173295No Hit
GCATAACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGC5140.11253100623299173No Hit
GTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATT5120.11209314239550927No Hit
GGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACA5100.11165527855802682No Hit
CAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGT5080.11121741472054435No Hit
ATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGT5060.11077955088306189No Hit
GAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGG5040.11034168704557944No Hit
GTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG5020.10990382320809698No Hit
GCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGT5020.10990382320809698No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT5010.10968489128935575No Hit
AATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAA5000.10946595937061453No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG4980.10902809553313207No Hit
CTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACA4900.10727664018320222No Hit
CAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA4870.10661984442697854No Hit
CTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACA4830.10574411675201363No Hit
ATCATAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGC4820.1055251848332724No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG4770.10443052523956625No Hit
ATATTATACTGCTGCCCACGCAATGAGTTGGTGTTTACAGTGTTCCCAAG4660.10202227413341275No Hit
CATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGAC4650.1018033422146715No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA4610.10092761453970658No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGAA203.8646982E-4108.751
ATAGAGC1100.0105.4545443
AAGGGTA1400.0103.571432
AGAGCCA1350.091.296295
ATTTTTG10350.091.0628051
TAGAGCC1400.088.035714
TGATAGA1350.085.925921
GCCATGC1350.085.925928
GATAGAG1350.085.925922
TTGGGTG11400.084.5833365
TGTTGTC8300.082.108433
GGGTAGT3500.080.785711
ATGATCC900.080.555564
TTTTGGG12150.080.555563
GAAGGGT1800.080.555561
GGGTGTG8850.073.728817
CTTAAGC300.001935066772.58
CATTGGG300.001935066772.57
AGCCATG1600.072.57
TTAAGCA300.001935066772.59
Produced by FastQC (version 0.11.5)