FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_32_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_32_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326644
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT10690.3272676063237041No Hit
AATTATGACCAACCAACTTTCGATCTCTTGTCTGAAGACATGCTTAACCC10360.3171648645008021No Hit
CCCTAATTATGACCAACCAACTTTCGATCTCTTGTCTGAAGACATGCTTA10130.310123559593931No Hit
GCTTAACCCTAATTATGACCAACCAACTTTCGATCTCTTGTCTGAAGACA8100.24797639019850357No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG7730.23664907360918921No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG6720.20572856075727702No Hit
ATCCAATATTGTAGCCACCAACCACATGTGACTATTGAGCACTTGAAATG6220.1904213761771225No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC6140.18797222664429777No Hit
CTGATATAGTTGTCACTAGCTACATGTGATCATTCATCTAAATTCAAATG5670.1735834731389525No Hit
TGCTTAACCCTAATTATGACCAACCAACTTTCGATCTCTTGTCTGAAGAC5630.17235889837254015No Hit
ATAATACCCATAGCAAAAGGTAAAAAGGCATTTTCATACAAAAAAAAGAA5290.16195001285803504No Hit
TATCTCTACTATCCAATATTGTAGCCACCAACCACATGTGACTATTGAGC5220.1598070070168134No Hit
AGCTACATGTGATCATTCATCTAAATTCAAATGAATTGCAGCTAAATCAC5170.15827628855879797No Hit
CCTATATTGAGCACGTACTGAGAGCTAGGAACTGAGCTGGATCCCTCAAG4980.15245955841833922No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT4790.1466428282778805No Hit
GATTATAAGAGCCCACATGGAAATGGCTTGATCTAAAGCATTACCATAAT4790.1466428282778805No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA4380.1340909369221538No Hit
ATATTGAGCACGTACTGAGAGCTAGGAACTGAGCTGGATCCCTCAAGGAG4370.1337847932305507No Hit
TTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA4270.13072335631451978No Hit
GGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGC4260.1304172126229167No Hit
GGTGGAGGGAGAGCGGCAGAGGTAGTTTTCGTTTTTAATGGCTTCCTTTC4260.1304172126229167No Hit
CTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGA4210.12888649416490125No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC4160.1273557757068858No Hit
CTTGTTTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT4140.1267434883236796No Hit
ATTCATAAGTGTCCACACTCTCCTAGCACCATCATCATACACAGTTCTTG4010.12276362033283943No Hit
CATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAA3920.12000832710841161No Hit
GTCTAAAGTAGCGGTTGAGTAAACAAAAGAGGCCAAAGTAACAAGTACAA3910.11970218341680852No Hit
GTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTT3900.11939603972520542No Hit
CTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTT3840.11755917757558687No Hit
CACTAATGCTGAACTCAAGAGGAGACCTCTTCCTCAGTGTTTGACGCTTT3800.11633460280917451No Hit
ACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAG3730.11419159696795288No Hit
CACCTATATTGAGCACGTACTGAGAGCTAGGAACTGAGCTGGATCCCTCA3730.11419159696795288No Hit
GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT3650.11174244743512815No Hit
GTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAA3630.11113016005192197No Hit
ATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCT3630.11113016005192197No Hit
ACATAACACAGTCTTTTAGCTTAAAACCAGACAAACTAGTATCAACCATA3620.11082401636031888No Hit
CATAAAAACAATACCTCTGGCCAAAAACATGACAGTTGTAACTACACCTG3620.11082401636031888No Hit
CTCCATATCTCTACTATCCAATATTGTAGCCACCAACCACATGTGACTAT3600.11021172897711269No Hit
GTCTAACAACATCAAAAGGTGTAAATTCATCTTCTAATAAAGCACTACCC3570.10929329790230344No Hit
GGCATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAA3540.10837486682749416No Hit
GACCAGAACTGTCCGGTAGAACTTTCTTTGATGATGGAAATACTCCATAT3490.1068441483694787No Hit
ATCTAAAGCATTACCATAATAAACTTTATAAACGAGTGTCAAGACATTCA3370.10317042407024161No Hit
GTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCAT3310.10133356192062305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAG651.5606929E-978.076922
CGACATA300.001934571372.56
CTAATGC1350.069.814823
CCGTGAC1009.094947E-1265.253
GCTGAAC1450.065.000018
CTTAGGG954.5474735E-1061.052635145
GATTAAT606.7953715E-660.4166684
TTGGCCG400.006047252654.3754
GTCTAGG400.006047252654.3751
CGGGGCG400.006047252654.3759
GTAGAGC953.1306627E-853.4210553
TTATATG2350.052.4468081
GGCCGTG1258.913048E-1152.21
TTGATTA1056.8874215E-848.3333322
TAGAGTT1650.048.3333328
GTACATA901.3208155E-648.3333321
GGACTTA450.00963337248.3333326
CCTAATG604.9401587E-448.333332145
GTGGATC450.00963337248.3333327
GTACTAA450.00963337248.3333321
Produced by FastQC (version 0.11.5)