FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_31_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_31_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466752
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAAT269265.768802276155218No Hit
GGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTC232144.973519127930893No Hit
CTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTG219154.695212875359934No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATA193574.1471702317290555No Hit
GTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCA107312.2990795968737143No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGT87121.8665158371040724No Hit
ATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCC80991.7351827094474155No Hit
ATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATT79621.7058309337721103No Hit
CCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACT76821.6458419031948441No Hit
TCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACA70911.5192221993692583No Hit
GTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA56301.206208007678596No Hit
AGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCA56131.2025658165364046No Hit
CAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTG49521.060948855066502No Hit
GTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAA49101.0519505004799123No Hit
GTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGT47031.0076014671602906No Hit
TCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGT46180.989390511449335No Hit
ACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGT44820.9602529823118058No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTG44160.9461127108185932No Hit
GGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGA43540.9328294254764844No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTG42430.9090480597833539No Hit
CTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTT38160.8175647881530234No Hit
CATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCT36080.773001508295626No Hit
CAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGT34990.7496486356780474No Hit
GCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC34910.7479346633758399No Hit
CTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGA28440.6093171534348005No Hit
GAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACA27300.5848930481283422No Hit
CACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCA26660.5711812697106815No Hit
AACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAG25580.548042643630879No Hit
ATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATT25190.5396870286576169No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCA24250.5195478541066776No Hit
GAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACC22910.4908388180447004No Hit
TGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGC22420.4803407376936789No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG21840.4679144385026738No Hit
CCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGAC21730.46555772658713834No Hit
TTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCG21140.45291718085835736No Hit
CCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGT19640.42078020019196494No Hit
GTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAA19440.4164952694364459No Hit
CAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGAT19310.4137100644453585No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT18980.4066399286987522No Hit
GCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCA17800.3813588372411902No Hit
ATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGG16700.3577917180858357No Hit
CAATCCACAATAGCGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAG16540.3543637734814205No Hit
ATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGT16000.3427944604415193No Hit
CAATTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGT15900.34065199506375976No Hit
GTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA14330.3070152886329357No Hit
GGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAAT13940.29865967365967366No Hit
CCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG13750.2945889894419306No Hit
GATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGC13390.2868761140819964No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT12100.2592383107088989No Hit
TGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTT12080.25880981763334704No Hit
TTACAAAATTGACCAATATTCCAGGCACCACCATGGGCCTCATAGCACCA11900.25495337995337997No Hit
GGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCAC11740.25152543534896477No Hit
GCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAA11100.23781365693130396No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT11050.23674242424242425No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA10980.2352426984779926No Hit
ACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGT10590.22688708350473058No Hit
GGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGC10530.22560160427807485No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC10470.22431612505141918No Hit
TTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA10430.22345913890031538No Hit
CAATTGGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAC10410.22303064582476348No Hit
CCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCTCCAAT10400.22281639928698754No Hit
CACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCA10370.22217365967365965No Hit
CACCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCG10200.21853146853146854No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT9650.20674790895379133No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT9580.20524818318935967No Hit
CGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAA9410.2016059920471685No Hit
CACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAG9370.20074900589606473No Hit
GGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACTGGTTC9220.19753530782942547No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC8780.1881084601672837No Hit
CAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC8770.18789421362950776No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT8700.18639448786507612No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA8410.18018133826957355No Hit
CTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTCTGTG8230.17632490058960648No Hit
CCCAAAAATGGATCATTACAAAATTGACCAATATTCCAGGCACCACCATG7720.16539832716303304No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT7520.16111339640751404No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG7470.1600421637186343No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG7370.1578996983408748No Hit
TTACAAAATTGACCAATATTCCAGGCACCACCATGGGCCTCATAGCACAT7190.15404326066090773No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC7110.15232928835870013No Hit
AGCAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAA7010.15018682298094063No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC6970.14932983682983683No Hit
CTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC6790.14547339914986973No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT6620.1418312080076786No Hit
CTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTT6420.1375462772521596No Hit
ATTTTTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAG6320.13540381187440012No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT6060.12983340189222542No Hit
ATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA6030.12919066227889758No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC5910.12661970382558618No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC5820.12469148498560263No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCC5810.12447723844782668No Hit
CAATACCAATGTGCTATGAGGCCCATGGTGGTGCTATGAGGCCCATGGTG5800.12426299191005073No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT5750.123191759221171No Hit
TGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC5580.11954956807897985No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC5570.1193353215412039No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG5420.11612162347456466No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC5410.1159073769367887No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA5320.11397915809680514No Hit
GTGCTATGAGGCCCATGGTGGTGCTATGAGGCCCATGGTGGTGCCTGGAA5240.11226518579459757No Hit
GTGCTATGAGGCCCAATGTGCTATGAGGCCCAATGTGCTATGAGGCCCAA5020.10755176196352667No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGACCAATATTCCAGGCAC4990.10690902235019882No Hit
TCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAG4800.10283833813245577No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT4780.10240984505690388No Hit
CACAATCCACAATAGCGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC4710.10091011929247223No Hit
ACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGA4710.10091011929247223No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGAA100.0070896586145.05
ATTGCCG100.0070896586145.0145
AGGGTAG306.493792E-10144.999983
CTATACC750.087.04
GCTATAC750.087.03
TGTTGTC4150.085.602413
TGCTATA801.8189894E-1281.56252
TGATGTA801.8189894E-1281.56251
GTGATGT554.3892214E-879.0909041
CTGTTGT4500.078.944452
TATACCA903.6379788E-1272.55
TATGGTA300.001935093872.499998
GGTAGTA608.022289E-872.499995
TTGTCTA5350.066.401865
GATGTAG1009.094947E-1265.252
GTTGTCT5550.064.009014
CCCTAGC9250.063.4864921
TAGCATT9250.062.7027054
CTAGCAT9250.062.7027053
TATATGG350.00356531262.1428576
Produced by FastQC (version 0.11.5)