FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_31_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_31_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476641
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGA90861.9062564907341164No Hit
AATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTG72921.5298725875449237No Hit
GCTTAACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAA68721.4417559546912666No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC67681.4199365979846468No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC63221.3263651259543345No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA57061.1971273977689707No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC56801.1916725585923158No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA55431.16292975216148No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT41830.8775997029210664No Hit
TGCTTAACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTA40720.8543117356668856No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG36930.7747969645917997No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC32040.6722040277693274No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG31790.6669589900994669No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA31220.6550003042121848No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA30190.6333907490123594No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC29890.6270967038085268No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG29480.6184948420299554No Hit
GAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTT27720.5815697768341372No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA25780.5408682845160194No Hit
TGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTG25540.5358330483529533No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA25410.5331056287646259No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA25010.5247135684928489No Hit
GATCACGCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC22750.4772984279573096No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA22470.4714239857670658No Hit
ATCACGCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACC20050.42065202112281574No Hit
ATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGT19820.415826586466544No Hit
CTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT19280.4044973050996452No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA17290.3627468052475553No Hit
GGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGG17060.3579213705912836No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT16710.3505783178534788No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA16040.33652161689825255No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC13530.28386143869285274No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA13180.27651838595504796No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT12980.2723223558191595No Hit
CCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTA12270.25742644883675553No Hit
GGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGG11780.24714617500382888No Hit
GTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT11230.2356070921301357No Hit
CTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGA11010.2309914589806584No Hit
AGATCACGCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACA10910.22889344391271416No Hit
ATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTC10770.22595622281759228No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA10000.20980150679442178No Hit
AATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTT9750.20455646912456127No Hit
ACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATG9590.2011996450158505No Hit
CAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTA9470.19868202693431744No Hit
ATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGA9390.19700361487996207No Hit
ACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAAC9270.194485996798429No Hit
ACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAA9230.1936467907712513No Hit
CTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTT8890.18651353954024097No Hit
TGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCA8730.18315671543153023No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC8710.18273711241794138No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC8560.17959008981602506No Hit
ATGCTTAACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGT8450.17728227324128643No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG7890.1655333888607988No Hit
ATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA7310.15336490146672233No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC7130.14958847434442274No Hit
TAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGAT7040.14770026078327295No Hit
ATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTC6740.1414062155794403No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC6690.1403572080454682No Hit
GCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGA6530.13700038393675745No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT6130.12860832366498057No Hit
GCTAGGGCCTGGAGATGCAGCCAGGGGAATGAGTTGGGAGCTAGGCCACC6080.12755931613100843No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC5990.12567110256985864No Hit
GGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCA5650.11853785133884831No Hit
GTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATAT5470.11476142421654872No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT5430.11392221818937104No Hit
ACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCT5350.11224380613501567No Hit
AATTATGACTGCACCAAAAACAAAGTATCCTTCCCGCAAACTGCAAGAAT5310.11140460010783798No Hit
GAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTG5290.11098499709424914No Hit
CTCCAGAGGTGGCTGGGCAGAGGTGGTGAGGCTCAGAGGCTTGGCTCAGG5290.11098499709424914No Hit
GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA5230.10972618805348261No Hit
GTGCTGGGGTGCCTGCTTCCATGTGGGTGTCACCACAGAGTGGAGGCAGC5120.10741837147874396No Hit
TTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTT5090.1067889669583607No Hit
GTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCAT4970.10427134887682762No Hit
GTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAG4950.10385174586323878No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC4870.10217333380888342No Hit
GGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATG4870.10217333380888342No Hit
ACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTG4820.1011243262749113No Hit
GTATAAGGAGCATGAGTTTCTTGCTAAGGCCTGGGCTGCCTGGGAGAAAG4800.10070472326132246No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCG100.007089706145.0145
TGCTTAA9050.096.132611
CCAGTCG259.383713E-487.09
CGTGGAC2550.076.76473
TCGTGGA2650.073.867922
CAGCGAT406.659612E-572.54
GTACTAA502.3043867E-672.51
CGATGAC406.659612E-572.57
GCTTAAC17850.072.296921
TTAACCC18100.071.298353
CTCGTGG2750.071.1818161
GGCCGTG1750.070.4285661
TAACCCT18450.070.338764
ACACGCA955.456968E-1268.684218
AACCCTA18800.068.6436165
CTTAACC19100.067.5654452
CATTGGC651.3964927E-766.923081
TACTAAT554.0583454E-665.9090962
GATCACG5200.062.7403871
ACAGCTA3200.061.1718758
Produced by FastQC (version 0.11.5)