FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_30_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_30_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611530
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC34560.5651398950174154No Hit
CCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGA33260.5438817392441908No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT31580.5164096610141775No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC30260.4948244566905957No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC29090.4756921164946936No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG28280.46244665020522296No Hit
GCTTAACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAA26490.4331758049482446No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG25000.4088106879466257No Hit
AATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTG24680.40357791114090885No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA23530.3847726194953641No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA23280.3806845126158978No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC22850.37365296878321586No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC21820.3568099684398149No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC20420.33391656991480384No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA17400.2845322388108515No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC15750.2575507334063742No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT15060.2462675584190473No Hit
TGCTTAACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTA14990.24512288849279673No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG14410.23563848053243505No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG14200.2322044707536834No Hit
GTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCATCTTGGACTG14160.2315503736529688No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT13970.22844341242457444No Hit
ATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCT13790.22549997547135875No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA13640.223047111343679No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT13440.21977662584010596No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC12330.20162543129527577No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG12300.20113485846973983No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA12080.1975373244158095No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT11920.19492093601295113No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC11420.1867447222540186No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC11270.18429185812633886No Hit
GAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTT11260.1841283338511602No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC11240.1838012853008029No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG10920.1785685084950861No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA10850.17742383856883553No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC10500.17170048893758277No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC10410.17022877046097493No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC10350.16924762480990302No Hit
CTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTA9970.16303370235311432No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG9950.162706653802757No Hit
TGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTG9770.1597632168495413No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA9750.159436168299184No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG9720.15894559547364806No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG9710.1587820711984694No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC9410.1538763429431099No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT9410.1538763429431099No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA9380.15338577011757396No Hit
CTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATT9100.14880709041257173No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA9010.14733537193596388No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC8970.1466812748352493No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA8810.1440648864323909No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT8500.13899563390185274No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG8450.1381780125259595No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC8290.13556162412310108No Hit
CCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGA8190.13392638137131457No Hit
CATCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAAT8030.13130999296845616No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA8030.13130999296845616No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT8020.13114646869327753No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG7910.12934770166631238No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT7820.1278759831897045No Hit
GCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTC7780.1272218860889899No Hit
GATCACGCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC7750.12673131326345397No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA7600.12427844913577421No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG7480.1223161578336304No Hit
ATCACGCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACC7330.11986329370595064No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG7240.1183915752293428No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC7110.11626575965202034No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC7060.1154481382761271No Hit
ATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCATC7020.1147940411754125No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT7000.11446699262505518No Hit
GTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTG6990.11430346834987654No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA6810.11136003139666083No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC6700.10956126436969568No Hit
GGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACC6690.10939774009451704No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA6610.10808954589308782No Hit
GGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGG6540.10694487596683727No Hit
ACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATA6490.10612725459094403No Hit
CTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAA6440.10530963321505077No Hit
GTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGACCC6430.10514610893987214No Hit
GGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCATCTTG6390.10449201183915752No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGG6380.10432848756397888No Hit
ATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAA6360.10400143901362158No Hit
CCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAG6310.10318381763772833No Hit
CTATTAGCATAAGCAGTTGTGGCATCTCCTGATGAGGTTCCACCTGGTTT6280.10269324481219236No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT6250.10220267198665642No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC6140.10040390495969127No Hit
ATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGT6130.1002403806845126No Hit
CATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAA6130.1002403806845126No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC100.0070901923145.000025
TTATCCG100.0070901923145.00002145
TTGGCCG700.093.2142944
TCGTGGG353.436206E-582.85715145
TGCTTAA4400.079.0909041
CTTAACC8250.078.212112
TTAACCC8450.076.360943
TTTCGAT1550.074.838712
AACCCTA8750.074.571435
GCTTAAC8900.073.314611
TTGCGCT300.001935381772.59
GATTGCG300.001935381772.57
GCATCCC300.001935381772.59
ATTGGCC955.456968E-1268.684213
ACTTTCG754.8512447E-967.6666641
TCTGTTC8850.067.175149
CATAGGG3250.064.6923142
CTTTCGA1800.064.444451
TTCGATC1800.064.444453
CCGTGAC1200.060.4166683
Produced by FastQC (version 0.11.5)