FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_29_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_29_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681341
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTAC27550.40434965751363855No Hit
GTCTTATTACAGTAGGCTAAGATAAGTGCACAAAAGTTAGCAGCTTCACC18870.2769538307543506No Hit
GGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTTAAGT16740.24569195160719817No Hit
CCCTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAA16290.23908732925216597No Hit
GTTTAAACACCGTGTAACTATGTTAGTAGTTGTACTAACAACTTTGTTAA15440.22661193147043845No Hit
TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT15370.22558454577076675No Hit
GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG15260.22397008252842557No Hit
GTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGT15100.22162177235774744No Hit
GCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTAT14810.21736546017339337No Hit
GTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCA14110.20709160317667658No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA14010.20562390932000277No Hit
CTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTT13840.20312882976365726No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT13720.20136759713564864No Hit
GTATAATGTCTCCTACAACTTCGGTAGTTTTCACATTACACTCAAGAACG13110.19241466460993834No Hit
TCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG12860.1887454299682538No Hit
GGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTT12640.18551650348357138No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC12640.18551650348357138No Hit
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC12510.1836085014698954No Hit
CCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAA12080.17729741788619796No Hit
GGTTCACCTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA11420.16761063843215074No Hit
AGTTCATACTGAGCAGGTGGTGCTGACATCATAACAAAAGGTGACTCCTG11420.16761063843215074No Hit
ATGCTAGGGAGAGCTGCCTACTGGACCAATTTGTAATGATCCATTTTTGG11180.16408817317613353No Hit
GACTAGAATTGTCTACATAAGCAGCCATTAGATCTGTGTGGCCAACCTCT11020.16173986300545543No Hit
GTAATAAGCATCTTGTAGAGCAGGTGGATTAAACTTCAACTCTATTTGTT10830.15895124467777516No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC10460.153520777408082No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT10120.14853061829539102No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT9370.1375229143703373No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC9310.13664229805633304No Hit
GGGTAAGGAAGGTACACATAATCATCACCCTGTTTAACTAGCATTGTATG9070.13311983280031586No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA9060.13297306341464846No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG8870.1301844450869682No Hit
CGTATACACCAGGTATTTGGTTTATACGTGGCTTTATTAGTTGCATTGTT8740.12827644307329222No Hit
GGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCG8610.12636844105961625No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG8590.12607490228828147No Hit
GGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGA8540.12534105535994458No Hit
CAATACACCCAAAAATGGATCATTACAAATTGGTCCAGTAGGCAGCTCTC8400.12328628396060122No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG8390.12313951457493386No Hit
GTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTA8160.11976381870458404No Hit
CCTCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAGGTG8100.11888320239057976No Hit
CTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTT8030.11785581669090808No Hit
GTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTA7740.113599504506554No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT7690.11286565757821707No Hit
CTTATAACCAGTTAACTGGTTTAAATCATCAGCAAATTTGATATTATCAC7630.11198504126421278No Hit
CTATTAACAGCAGCTAAACCATGAGTAGCAAGGGTTTTCAAACCTAATAC7580.11125119433587588No Hit
CTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAAGA7530.11051734740753895No Hit
GTTTATAATCAATAGCCACCACATCACCATTTAAGTCAGGGAAAAATGTA7430.10904965355086514No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT7410.10875611477953037No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG7350.10787549846552606No Hit
TTCTTAAATTGTTCATAAGAAAGTGTGCCCATGTACATAACAGCTTCTAC7350.10787549846552606No Hit
CTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTG7290.10699488215152178No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT7240.10626103522318486No Hit
AACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAA7190.10552718829484795No Hit
CCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAGGTG7190.10552718829484795No Hit
CCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATC7170.10523364952351319No Hit
GATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGA7140.10479334136651104No Hit
GTTTTACACACCACGTTCAAGACTCTTTTGCAAGAATCTAAATTGGCATG7130.10464657198084365No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG7110.10435303320950891No Hit
GCCTACTGGACCAATTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTC7060.10361918628117198No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC7010.10288533935283507No Hit
ATTTATAAGTAACTGGTTTTATGGTTGTTGTGTAACTGTTTTCTTTGTAG6980.10244503119583293No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTCG151.2298347E-4145.05
AAGGGTA450.0145.02
ATACGAC100.0070903706144.999983
GAAGGGT500.0130.51
AGGGTAG550.0118.636373
GCCGTAC259.3854556E-487.0145
CATGAAG900.080.555566
ATGAAGG900.080.555567
ATTCCGC300.001935476772.56
GCAGGTC300.001935476772.58
GCCATGC3200.063.4374968
GGCAGGT350.00356601662.1428577
AGGCAGG350.00356601662.1428576
AATTCCG350.00356601662.1428575
GGGTAGT2150.060.6976781
AGCCATG3350.060.5970157
AGTAGAG2150.057.3255845
AGGTGGG902.048182E-856.38889145
TTCCGCA400.00605006354.3749967
TGGGGTG400.00605006354.3749965
Produced by FastQC (version 0.11.5)