FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_29_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_29_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508800
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC13500.2653301886792453No Hit
CTATATATGGAGCATCTTTCTTTAAGAAAGTGATGTCAATGTCACTAACA13430.2639544025157233No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC12510.24587264150943397No Hit
GTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTG12370.24312106918238993No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT12100.23781446540880502No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA11720.23034591194968554No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG10740.21108490566037735No Hit
CTCATATACCGAGCAGCTTCTTCCAAATTTAAGCCATGTGTTACATAGCC9880.19418238993710693No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG8730.1715801886792453No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA8680.17059748427672958No Hit
CCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGT8510.1672562893081761No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC8450.16607704402515724No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC8300.16312893081761007No Hit
GTATAGTTGAAACTATGGCTTTAGTTTCCACACAGACAGGCATTAATTTG8140.15998427672955975No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA7950.15625No Hit
CTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC7800.15330188679245285No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT7490.14720911949685533No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC7480.1470125786163522No Hit
CTCTTAAGAAGTTGAATGTCTTCACCTTTGTTAACATTTGGGCCGACAAC7430.14602987421383648No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT7370.14485062893081763No Hit
CCCTTATATTTACGCTGTATAGTTGAAACTATGGCTTTAGTTTCCACACA7210.14170597484276728No Hit
GTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAAT7120.139937106918239No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG6960.13679245283018868No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC6900.1356132075471698No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC6630.1303066037735849No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC6410.12598270440251572No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA6400.12578616352201258No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA6260.12303459119496855No Hit
GTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCATCTTGGACTG6140.12067610062893083No Hit
CCATTATACGCTGTAACAGCATCAGGTGAAGAAACAGAAACTGTAGCTGG6050.11890723270440251No Hit
GTCCAGAATAGGACCAATCTTTATAGGAACCAGCAAGTGAGATGGTTTCA5930.11654874213836477No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG5890.11576257861635221No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC5850.11497641509433962No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT5840.11477987421383648No Hit
CTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCTGATAATAAT5820.11438679245283018No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG5740.11281446540880505No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC5650.11104559748427673No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG5590.10986635220125787No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT5450.10711477987421383No Hit
GTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTA5420.10652515723270439No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT5400.10613207547169812No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT5380.10573899371069183No Hit
GTATCTACACAAACTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACC5370.10554245283018868No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG5350.10514937106918239No Hit
ATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGAT5240.10298742138364779No Hit
ATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAG5150.1012185534591195No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCCC100.007089847144.99998145
ACTTTCG100.007089847144.999981
CAGGTAT203.8649543E-4108.749994
TTTCGAT553.6379788E-12105.4545442
CTTTCGA607.2759576E-1296.6666641
TTGGCCG259.3840784E-487.04
ATATGCG259.3840784E-487.09
GTATATA851.8189894E-1276.764711
ATTCAGC300.00193519472.51
ATAGGGA809.822543E-1172.499991
AACCCTA1150.069.347835
TCATTCG851.6916601E-1068.23538
CTTAACC1200.066.4583362
ATAGGCG451.1932967E-464.444447
TTAACCC1350.059.0740743
GGATTAA753.7575046E-758.01
CAGACTA902.0469088E-856.388894
GGGAAGT1051.1077645E-955.2380944
AAATTAG4350.053.3333325
CATTCGT1101.6734703E-952.7272729
Produced by FastQC (version 0.11.5)