FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_28_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_28_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325722
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCAC19940.6121784834920576No Hit
CTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGCAAAGCACTCGTG19500.598670031499254No Hit
GCTAGACCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGGACA19050.5848545692338866No Hit
CCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCAC15280.46911169647736417No Hit
GTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCAC15210.46696262456941806No Hit
GTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCATCAATTTC14870.45652427530225165No Hit
CATGTAGAGAGACCACATATAGAGAAAGATGCCAGAGGGATGCCAGCTGT13600.41753397068665915No Hit
GCTTTTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGAC13500.41446386796102197No Hit
GTTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGC13040.4003413954230909No Hit
GGGTACGAGTGCTTTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCAC12710.3902100564284881No Hit
TCCCTATGGTGCTAGCTGTCCACGAGTGACACCACCATCAACCAACCAAC11370.34907067990494967No Hit
GGTTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAG10750.330036043005999No Hit
CTTGTGTTCTCAATTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCA9800.30087006711244557No Hit
GTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCATCAACCAA9510.2919667692080977No Hit
CCATAGGGAAGTCCAGCTCTGAAGACATGCTTAACCCTAATTATGTCACG8740.2683269782206913No Hit
CTCGTACCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGGACA8650.2655638857676178No Hit
CCATAGGGAAGTCCAGCTATCCTGAAGCCAACATGTAGAGAGACCACATA8610.26433584467736293No Hit
CTTGTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACA8230.25266945431994153No Hit
GCTTAACCCTAATTATGTCACGAGTGACACCACCATCAATTTCCATTTGA7940.24376615641559365No Hit
CTCGTGGACAGCTAGACCCAGGAGTCAAATGGAAATTGATGGTGGTGTCA7620.23394182769355462No Hit
GTCCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACC7390.22688059142458908No Hit
CCATAGGGAAGTCCAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGT7340.22534554006177046No Hit
GTCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCA6980.21429317024947653No Hit
GGGTACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCATCAACCAA6940.21306512915922168No Hit
TCCCTATGGTGCTACAAGAGATCGAAAGTTGGTTGGTTGATGGTGGTGTC6750.20723193398051099No Hit
CTCGTGGACAGCTAGCAAAGCACTCGTACCCAGGAGTCAAATGGAAATTG6710.20600389289025609No Hit
GTCCAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGCTAG6620.20324080043718262No Hit
TCCCTATGGTGCTGGACTTCCCTATGGTGCTAGCTGTCCACGAGTGACAC6420.1971005949859082No Hit
GCTAGCAAAGCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGC6380.19587255389565336No Hit
CATATAGAGAAAGATGCCAGAGGGATGCCAGCTGTCCTAGCTGTCCACGA6380.19587255389565336No Hit
CCATAGGGAAGTCCAGCCCTCAAATTCCAAGGTCCCACTAGCTGTCCACG6380.19587255389565336No Hit
GTCCACGAGTGACACCACCATCAATTGAGAACACAAGAGATCGAAAGTTG6330.19433750253283474No Hit
CTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGACCCAGGAGTCAA6210.1906533792620701No Hit
TCCCTATGGTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTG6170.18942533817181523No Hit
GCTAGCAAAGCACTCGTACCCAGGAGTCAAATGGAAATTGATGGTGGTGT6160.1891183278992515No Hit
ACCCTAATTATGTCACGAGTGACACCACCATCAATTTCCATTTGACTCCT5920.18175008135772222No Hit
CTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTA5900.18113606081259478No Hit
CCCTAATTATGTCACGAGTGACACCACCATCAATTTCCATTTGACTCCTG5880.18052204026746735No Hit
GTGCTGGACTTCCCTATGGTGCTAGCTGTCCACGAGTGACACCACCATCA5790.17775894781439386No Hit
GTCCACGAGTGACACCACCATCAATTTCCATTTGACTCCTGGGTCTAGCT5590.17161874236311947No Hit
GCTAGGACAGCTGGCATCCCTCTGGCATCTTTCTCTATATGTGGTCTCTC5550.1703907012728646No Hit
CTTTGCTAGCTGTCCACGAGTGACACCACCATCAATTTCCATTTGACTCC5410.16609255745697252No Hit
GTCCACGAGTGACACCACCATCAATTTCCATTTGACTCCTGGGTACGAGT5280.16210142391364415No Hit
GTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCATCAATTGA5100.1565752390074972No Hit
GTGCTAGCTGTCCACGAGTGACACCACCATCAACCAACCAACTTTCGATC5050.15504018764467858No Hit
GTGTTCTCAATTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTC5040.15473317737211487No Hit
ATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGC4980.15289111573673256No Hit
CTTGTGTTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGG4920.15104905410135025No Hit
GTTGATGGTGGTGTCACTCGTGGACAGCTAGCACCATAGGGAAGTCCAGC4910.15074204382878653No Hit
GGGTCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGCTTTGCT4820.14797895137571304No Hit
GTCCAGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACC4760.1461368897403307No Hit
TGCTTAACCCTAATTATGTCACGAGTGACACCACCATCAATTTCCATTTG4680.14368080755982093No Hit
GCTAGCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGGACAGC4630.14214575619700237No Hit
GTGCTACAAGAGATCGAAAGTTGGTTGGTTGATGGTGGTGTCACTCGTGG4630.14214575619700237No Hit
CTTTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCA4580.14061070483418375No Hit
GGTCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACC4560.13999668428905632No Hit
GTGCTTTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACA4540.13938266374392888No Hit
GCTCTGAAGACATGCTTAACCCTAATTATGTCACGAGTGACACCACCATC4520.13876864319880144No Hit
GCAACAGCCTGCCTTTTCTGGGTTGAGGCTGATGGTGGTGTCACTCGTGG4490.13784761238111026No Hit
GTCAAATGGAAATTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCAC4450.1366195712908554No Hit
CAGCTAGACCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGGA4290.13170740692983587No Hit
CTCGTGGACAGCTAGCCCAGGAGTCAAATGGAAATTGATGGTGGTGTCAC4250.130479365839581No Hit
TTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACC4220.12955833502188982No Hit
GCTAGAGGGTGTGGTCACACAGGGAAGAGCCACTGGAGAACAAAGGCTGG4150.1274092631139438No Hit
GTCCACGAGTGACACCACCATCAACCAACCAACTTTCGATCTCTTGTCAC4120.1264882322962526No Hit
CTTTGCTAGCTGTCCACGAGTGACACCACCATCAACCAACCAACTTTCGA3930.12065503711754197No Hit
CTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGCACCATAGGGAAG3910.12004101657241452No Hit
GGTTGATGGTGGTGTCACTCGTGGACAGCTAGCACCATAGGGAAGTCCAG3900.1197340062998508No Hit
TGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTA3890.11942699602728707No Hit
TCCCTATGGTGCTGGACTTCCCTATGGTGCTACCAACCAACTTTCGATCT3840.11789194466446846No Hit
TCCCTATGGTGCTACCAACCAACTTTCGATCTCTTGTGTTCTCAATTGAT3770.11574287275652242No Hit
GCTAGCAAAGCACTCGTGGACAGCTAGACCCAGGAGTCAAATGGAAATTG3690.11328679057601267No Hit
GCTAGCCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGGACAG3680.11297978030344895No Hit
CTCGTGGACAGCTAGCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACT3680.11297978030344895No Hit
GTCCAGCTATCCTGAAGCCAACATGTAGAGAGACCACATATAGAGAAAGA3680.11297978030344895No Hit
CAATTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACA3650.11205874948575781No Hit
CGTACCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGGACAGC3650.11205874948575781No Hit
TAGCAACAGCCTGCCTTTTCTGGGTTGAGGCTGATGGTGGTGTCACTCGT3620.11113771866806664No Hit
GGGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACC3600.11052369812293919No Hit
CTCTATATGTGGTCTCTCTACATGTTGGCTTCAGGATAGCTGGACTTCCC3590.11021668785037547No Hit
GTGCTTTGCTAGCTGTCCACGAGTGACACCACCATCAATTTCCATTTGAC3580.10990967757781174No Hit
GTCCACGAGTGACACCACCATCAACCAACCAACTTTCGATCTCTTGTGTT3580.10990967757781174No Hit
GGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGCAAAGC3570.10960266730524802No Hit
GTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGCAAA3550.1089886467601206No Hit
TTTCTGGGTTGAGGCTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGC3540.10868163648755688No Hit
CCAGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCAC3530.10837462621499315No Hit
GTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACC3520.10806761594242943No Hit
GGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCA3450.10591854403448339No Hit
GCTAGACCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGACAT3440.10561153376191967No Hit
CCATAGGGAAGTCCAGCAAGTACCATGTTCATAAAGATTGGCCTGACCCT3350.1028484413088462No Hit
GGTGCTAGCTGTCCACGAGTGACACCACCATCAACCAACCAACTTTCGAT3340.10254143103628248No Hit
GCTACTGTCAGGTGGGCACAGCAGGTGTGGAGGAGAACATGCAACGGGAG3310.10162040021859131No Hit
GCTAGAGTGAACGGGAGGAAGGTGACAGGGACGAGGCCAGTGACAAGAGA3280.10069936940090016No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCG100.0070886835144.99998145
CTAATTG100.0070886835144.999983
GGGATTG100.0070886835144.99998145
CAGTTGT458.367351E-11112.777781
CTAATAG900.0112.777781
TAATAGC950.0106.8420942
TGTTCAG501.9463187E-10101.55
AATAGCA1100.098.863633
CGGAGAT301.5978325E-596.6666645
GTACTAA1250.087.01
CACTAAC608.276402E-1084.5833367
GATGTGT353.433791E-582.857149
CTCGGAG353.433791E-582.857143
ACTAGCA1600.081.562492
TGCCTTA1600.081.562496
GTTGTTC651.5606929E-978.076933
TACTAAT1400.077.678572
GCACTAA755.638867E-1177.333336
TAGAGCA502.3027442E-672.54
GTACAAC300.001934566572.54
Produced by FastQC (version 0.11.5)