FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_27_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_27_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381169
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGG81632.1415697499009627No Hit
CCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCACCAATATT56741.4885785570180157No Hit
GGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGG47341.2419687854993455No Hit
ATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCA46471.2191442640928303No Hit
CCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCAC44781.1748069753836223No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTG34660.9093079447699052No Hit
GCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCA33200.8710047249382821No Hit
TTACAAAATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAG26240.6884085536861602No Hit
ATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTTGGGTGT25310.6640099273550577No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGGTAGGCAGCTCTCCCTA24000.6296419698349026No Hit
CCCTGTTCACCAATATTCCAGGCACCCTGTTTGCGCATCTGTTCACCAAT23380.6133762189475009No Hit
GTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCACCA21640.5677271761344705No Hit
AAATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC21370.5606437039738279No Hit
CCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTGCGCATCTGT19460.5105346972078002No Hit
GGGTGCCTGGAATATTGGTGAACAGGGTGCCTGGAATATTGGTGAACAGA17790.46672211014012155No Hit
GGTTCAGGGCAATTTTGTAATGATCCATTTTTGGGTGTGGGTGCCTGGAA16340.42868124112926287No Hit
GGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGGTGCCT15740.4129401918833904No Hit
TTGGTGAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGTGAA15590.4090049295719222No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGGGCAATTTTGTAATGA15440.405069667260454No Hit
CCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCAC14490.38014633928782243No Hit
CCCCTATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA14380.3772604802594125No Hit
ATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCA12880.3379078571447311No Hit
GTGCTATGAGGCCCAGAAGGTGCCTGGAATATTGGTGAACAGGTGCCTGG12880.3379078571447311No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTGCG12290.3224291587196231No Hit
CCTGTATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA12050.31613273902127403No Hit
CCCACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTGCGC11790.3093116176813959No Hit
CAATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTTGGGT11330.29724347992622696No Hit
GGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGG10750.2820271323218835No Hit
ATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTG10620.2786165716519444No Hit
CCAATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTTGGG10300.27022134538747905No Hit
CAGGGTGCCTGGAATATTGGTGAACAGGGTGCCTGGAATATTGGTGAACA10140.2660237322552464No Hit
GGTGAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGTGAACA10130.2657613814344818No Hit
CAGGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGT10110.26523667979295273No Hit
CAATGTGCTATGACCAACGGTGCCTGGAATATTGGTGAACAGATGCGCAA10040.26340022404760094No Hit
CCCCCATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA9650.2531685420377838No Hit
CCCCTATTGTGGGCTCAATGTGTCCAGGCACCACACCCAAAAATGGATCA9640.25290619121701924No Hit
CAATTGGTGCCTGGAATATTGGTGCCTGGAATATTGGTGAACAGATGCGC9610.2521191387547256No Hit
CCCTAGCATTGGTGCCTGGACACATTGAGCCCACAATACACCCAAAAATG9430.24739682398096383No Hit
ATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCT9210.24162510592414388No Hit
GGTTCAGGGCAATTTTGTAATGATCCATTTTTGGGTGTCTGGACACATTG8640.22667110914056493No Hit
CCAATATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA8600.22562170585750677No Hit
GTGGGCCCAATGTGCTATGAGGCCCAGAAGGTGCCTGGAATATTGGTGAA8480.22247349600833227No Hit
GAATATTGGTGAACAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACA8380.21984998780068682No Hit
TCTGTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCA8360.21932528615915772No Hit
CAATAGGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGG8090.2122418139985151No Hit
CAGGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA8010.21014300743239875No Hit
CCAATATTCCAGGCACCCTGTTCACCAATATTCCAGGCACCCTGTTTGCG7890.20699479758322423No Hit
CCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCTGTTC7860.20620774512093062No Hit
CCCTAGCATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTG7850.2059453943001661No Hit
CTGTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCAC7760.20358423691328517No Hit
CCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTGTTCA7710.20227248280946247No Hit
GCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCTGTT7680.20148543034716881No Hit
GCCTACCAATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTCAATG7580.19886192213952342No Hit
CCTGTATTGTGGGCTCAATGTGTCCAGGCACCACACCCAAAAATGGATCA7430.19492665982805527No Hit
CCAATATTCCAGGCACCTGTTCACCAATATTCCAGGCACCTTCTGGGCCT7350.1928278532619389No Hit
ATTGGTAGGCAGCTCTCCCTAGCATTGGTGCCTGGACACATTGAGCCCAC7140.18731848602588352No Hit
GAATATTGGTGAACAGATGCGCAAACAGGGTGCCTGGAATATTGGTGAAC7110.18653143356358992No Hit
GCCCAGAAGGTGCCTGGAATATTGGTGAACAGGTGCCTGGAATATTGGTG7090.18600673192206083No Hit
CACCAATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTTG7070.18548203028053173No Hit
ATTGGTAGGCAGCTCTCCCTAGCATTGAGCCCACAATACACCCAAAAATG7020.184170276176709No Hit
CCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCAC6940.18207146961059267No Hit
CCCAATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTTGG6770.17761150565759545No Hit
CAATAGGGGTGCCTGGAATATTGGTGCCTGGAATATTGGTGAACAGATGC6670.17498799744995003No Hit
CCCTAGCATTGTGGGCCCAATGTGCTATGAGGCCCAGAAGGTGCCTGGAA6640.1742009449876564No Hit
CCCCCATTGTGGGCTCAATGTGTCCAGGCACCACACCCAAAAATGGATCA6600.17315154170459823No Hit
CATCTGTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTT6580.17262684006306914No Hit
GGTTCAGGGCAATTTTGTAATGATCCATTTTTGGGTGTGGTGCCTGGACA6500.1705280334969528No Hit
ATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGG6490.17026568267618825No Hit
GGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGG6420.16842922693083645No Hit
GCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTGTTC6330.16606806954395556No Hit
CAATACAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGG6220.1631822105155456No Hit
CAATGGGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGG6170.1618704564117229No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGGGCAATTTTGTAATGATCCAT6140.16108340394942927No Hit
GGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGCCTG6110.1602963514871356No Hit
CCACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTGCGCA6080.15950929902484198No Hit
CAATCGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGGGT6000.15741049245872565No Hit
CCAATATTCCAGGCACCCTGTTTGCGCATCTGTTCACCAATATTCCAGGC5830.15295052850572843No Hit
ATTTTGTAATGATCCATTTTTGGGTGTGGGTGCCTGGAATATTGGTGAAC5600.14691645962814393No Hit
GGGCCCAATGTGCTATGAGGCCCAGAAGGTGCCTGGAATATTGGTGAACA5490.14403060059973397No Hit
CAATCAATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTT5470.14350589895820487No Hit
GAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGTGAACAGAT5380.141144741571324No Hit
GTCCAGACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTG5260.13799653172214948No Hit
CAATTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGGGT5250.13773418090138495No Hit
CCAATATTGTGGGCTCAATGTGTCCAGGCACCACACCCAAAAATGGATCA5110.13406126941068136No Hit
CACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCACCAATA5060.13274951530685863No Hit
CCAATATTCCAGGCACCCACACCCAAAAATGGATCATTACAAAATTGCCC5050.1324871644860941No Hit
CCCATATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA5020.13170011202380047No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCT5010.1314377612030359No Hit
CAATATTCCAGGCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCAC4970.13038835791997777No Hit
CCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACACC4920.12907660381615504No Hit
CCCAAAAATGGATCATTACAAAATTGGTAGGCAGCTCTCCCTAGCATTGT4910.12881425299539048No Hit
CAATATTGGTGCCTGGAATATTGGTGCCTGGAATATTGGTGAACAGATGC4900.12855190217462595No Hit
GGCCCAATGTGCTATGAGGCCCAGAAGGTGCCTGGAATATTGGTGAACAG4870.12776484971233235No Hit
GTCCAGGCACCACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCT4810.12619074478774506No Hit
CCAATATTCCAGGCACCAATGCTAGGGAGAGCTGCCTACCAATTTTGTAA4760.12487899068392236No Hit
CAATCCCAATGTGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAG4710.12356723658009965No Hit
GTGAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGTGAACAG4690.12304253493857055No Hit
GCATTGGTGCCTGGACACATTGAGCCCACAATACACCCAAAAATGGATCA4650.12199313165551237No Hit
CCTACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCAC4560.1196319742686315No Hit
GTTCAGGGCAATTTTGTAATGATCCATTTTTGGGTGTCTGGACACATTGA4400.11543436113639882No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGC4390.11517201031563427No Hit
GGTGAACAGATGCGCAAACAGGTTCAGGGCAATTTTGTAATGATCCATTT4390.11517201031563427No Hit
CAATACCAATGTGCTATGAGGCCCAGAAGGTGCCTGGAATATTGGTGAAC4340.11386025621181156No Hit
CCCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCACCTGTTT4300.11281085292875338No Hit
ATATTGGTGAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGT4300.11281085292875338No Hit
CCACCCATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTAC4280.11228615128722431No Hit
ATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAA4250.11149909882493067No Hit
CCCAAAAATGGATCATTACAAAATTGACACCCAAAAATGGATCATTACAA4170.10940029225881434No Hit
GAATATTGGTGAACAGGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC4150.10887559061728526No Hit
GGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACG4120.10808853815499161No Hit
CCCTAGCATTGTGGGACCAATGTGCTATGAGGCCCAGAAGGTGCCTGGAA4100.10756383651346253No Hit
GTTCAGGGCAATTTTGTAATGATCCATTTTTGGGTGTGGGTGCCTGGAAT4050.10625208240963983No Hit
CAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCG4040.10598973158887529No Hit
GCATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTGGTAGG4040.10598973158887529No Hit
GCCCACAATACACCCAAAAATGGATCATTACAAAATTGGTAGGCAGCTCT3950.10362857420199438No Hit
CACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCACCT3930.10310387256046531No Hit
CCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAATATT3890.10205446927740713No Hit
ATTGCATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA3880.10179211845664259No Hit
AGCAACAGATGCGCAAACAGGTTCAGGGCAATTTTGTAATGATCCATTTT3860.1012674168151135No Hit
GGCACCACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTG3850.10100506599434896No Hit
CCAATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACAAA3840.10074271517358441No Hit
ATTGGTAGGCAGCTCTCCCTAGCATTGGTGCCTGGAATATTGGTGAACAG3830.10048036435281987No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTTT100.0070891525145.0145
CTATTGA100.0070891525145.04
GTATGAC352.758443E-7103.571432
TATGACA406.1101673E-790.6253
GTGATGT259.3822606E-487.01
GATGTAG451.2314176E-680.555562
TGATGTA451.2314176E-680.555561
ATGACAC502.303499E-672.54
CAATTAC651.3957288E-766.923071
GGTAACA1350.064.444441
AATCGGT3850.064.025982
TCGGTGC3900.063.2051284
GTAACAC1400.062.1428572
CAATCGG4000.061.6251
CCCCTAT9100.061.346151
CCGTGAA10650.061.2676051
TAGGGGT5550.060.090094
CGTGAAC10950.059.5890432
CCCCATT5350.058.2710272
GGTATGA753.7549944E-758.01
Produced by FastQC (version 0.11.5)