FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_27_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_27_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683686
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGG469666.869527824176595No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGA442916.478266338640839No Hit
GGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCAC255123.731537577191869No Hit
GTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCC233703.41823585681146No Hit
GTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTG200252.9289761674218866No Hit
GTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAG174882.5578993865604973No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGAC169792.483450004826777No Hit
CAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGAC154312.2570302741316923No Hit
GCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCT92741.356470660507894No Hit
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCT92141.347694701953821No Hit
AGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGC87151.2747079799791132No Hit
GTCCAGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCA86211.2609589782443986No Hit
CCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATA81851.1971870127514679No Hit
GCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTT76291.1158631301503907No Hit
GCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGG66510.9728150057189997No Hit
CTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGC65010.950875109333817No Hit
GCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAG57800.845417340709039No Hit
GGCTAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGCTA47770.6987125668801174No Hit
GTGCTAGCCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATGGTGCT47190.6902291402778469No Hit
GTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTA46490.6799905219647616No Hit
ATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAG46240.6763338725672312No Hit
CAGCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCT44200.6464956134833827No Hit
GGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCC38870.5685358483280336No Hit
GTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTAGCTGTCCA34940.511053319798855No Hit
GAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAG33290.48691943377515406No Hit
CTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTAT30960.45283946139017034No Hit
GGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCC30400.44464856673970216No Hit
CAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCT28380.41510283960765615No Hit
CCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGT23870.34913688447620694No Hit
CTCGTGGACAGCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGT23560.34460263922326917No Hit
GTCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTG22710.3321700312716656No Hit
CGATAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCAC22630.3309999034644559No Hit
GCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAG22140.32383287064529626No Hit
GCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCAC21090.30847494317566837No Hit
GTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGG20430.29882138876618797No Hit
GCTTTGGCTAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTT18810.2751263006701907No Hit
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCTTATGG18780.27468750274248704No Hit
GGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGC18780.27468750274248704No Hit
GCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTG18530.2710308533449566No Hit
TCCCTATGGTGCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGT17900.2618160968631799No Hit
CACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGC17520.2562579897789336No Hit
CTTGTGATGGTGGTGTCACTCGTGGACAGCTGGACTTCCCTATGGTGCTA17230.2520162764777983No Hit
GTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGC16920.24748203122486054No Hit
CCAGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGC16520.24163139218881183No Hit
GTGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGAC16370.23943740255029355No Hit
CGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTT16130.23592701912866434No Hit
GTGCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCT15890.2324166357070351No Hit
CGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAG15810.23124650789982537No Hit
CCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCAC15720.2299301141167144No Hit
AAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGC15670.22919878423720833No Hit
AGCCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATGGTGCTAGCCA15440.2258346667914803No Hit
CCATAGGGAAGTCCAGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGT14840.21705870823740722No Hit
CCATAGGGAAGTCCAGCACCATAGGGAAGTCCAGCACCATAGGGAAGTCC14380.21033047334595123No Hit
CACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGG14290.20901407956284024No Hit
GCTTAACCCTAATTATGGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCAC14090.2060887600448159No Hit
AGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCT13990.20462610028580375No Hit
CCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTG13800.2018470467436806No Hit
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCAAAGCACTCGT13530.19789786539434767No Hit
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGACTTCCCTATGG13040.19073083257518803No Hit
TCCCTATGGTGCTGGACTTCCCTATGGTGCTGGACTTCCCTATGGTGCTG12990.18999950269568194No Hit
CAGCTAGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGAC12940.18926817281617586No Hit
GCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCTGG12270.17946835243079426No Hit
GTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCTAGCTG12240.1790295545030906No Hit
CAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCA12040.17610423498506625No Hit
CTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATG11780.17230131961163458No Hit
GGCTAGCACCATAGGGAAGTCCAGCTAGCACCATAGGGAAGTCCAGCTGT11630.1701073299731163No Hit
TTGTCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGC11570.169229734117709No Hit
GTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTAGCACCATA11460.1676208083827956No Hit
GCTTAACCCTAATTATGATGCTAGCTGTCCACGAGTGCTTTGGCTAGCAC11350.16601188264788222No Hit
GTGCTGGACTTCCCTATGGTGCTGGACTTCCCTATGGTGCTGGACTTCCC11260.16469548886477126No Hit
GTGCTATCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCG11220.16411042496116637No Hit
GTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCT10290.15050768920235313No Hit
GCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGCTAGCACC10240.14977635932284702No Hit
GCTAGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTT10150.1484599655397361No Hit
GTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCT9980.1459734439494154No Hit
GTCCAGCACCATAGGGAAGTCCAGCACCATAGGGAAGTCCAGCACCATAG9970.14582717797351416No Hit
CCCTAATTATGATGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGG9370.1370512194194411No Hit
TAATTATGGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAA9300.13602735758813256No Hit
GTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGTTAGCC9150.13383336794961428No Hit
CCCTAATTATGGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGG8910.13032298452798505No Hit
AGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCAC8800.12871405879307166No Hit
TCCCTATGGTGCTACCTATGGTGCTAAGCTGGACTTCCCTATGGTGCTAG8680.12695886708225707No Hit
AGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTC8650.1265200691545534No Hit
CTTGTGTGATGGTGGTGTCACTCGTGGACAGCTGGACTTCCCTATGGTGC8640.12637380317865218No Hit
CCATAGGGAAGTCCAGCTTAGCACCATAGGTAGCACCATAGGGAAGTCCA8570.12534994134734367No Hit
TAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGAC8560.12520367537144245No Hit
GTCCAGCACTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTC8450.12359474963652906No Hit
CTTGTTGATGGTGGTGTCACTCGTGGACAGCTGGACTTCCCTATGGTGCT8200.1199381002389986No Hit
GTCCAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCAC8140.1190605043835913No Hit
CTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACG8070.11803664255228277No Hit
AAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCG7990.11686651474507304No Hit
GCTAGTGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCT7900.11555012096196207No Hit
CTTGTGTTCTCAATTGATGGTGGTGTCACTCGTGGACAGCTGGACTTCCC7810.11423372717885112No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGT7790.11394119522704868No Hit
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCAAGGCACTACC7770.11364866327524623No Hit
CCATAGGGAAGTCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGA7750.11335613132344381No Hit
TCCCTATGGTGCTATCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAA7580.1108696097331231No Hit
CAGCTGGACTTCCCTATGGTGCTAGCTGGACTTCCCTATGGTGCTAGCCA7540.11028454582951823No Hit
GCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCA7430.10867562009460484No Hit
GTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCTACGAG7410.1083830881428024No Hit
GTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATA7330.10721296033559266No Hit
GGGTGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGG7310.10692042838379022No Hit
CAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGAC7310.10692042838379022No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCTGGACTTCCCTATGGT7250.10604283252838291No Hit
ACCCTAATTATGGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAG7200.10531150264887683No Hit
GGCTAGCACCATAGGGAAGTCCAGCTGTCCACGATAGCACCATAGGGAAG7180.10501897069707439No Hit
TGCTTAACCCTAATTATGGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCA7170.10487270472117316No Hit
CCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTG7170.10487270472117316No Hit
GCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGT7150.10458017276937075No Hit
ATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGG7090.10370257691396344No Hit
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGACAAAGCACTCG7070.103410044962161No Hit
CCCTAATTATGACCCAGGAGTCAAATGGAAATTGAACCAACCAACTTTCG6950.10165485325134638No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTTA100.0070903758145.04
TGTGTTG2700.061.7592623
CTTGATG1850.058.7837871
CTTAACC13500.051.5555532
TAACCCT13800.051.4855044
GCTTAAC13600.051.1764681
TTAACCC13800.050.9601443
ACCCTAA13700.050.8029176
AACCCTA14300.049.6853185
TGCTTAA8550.049.1812861
GTGTTGA3450.048.3333324
ATGACTC450.00963784448.3333323
ATGAAGA2400.048.3333321
GAAGACT2300.044.1304363
ATGACAT855.339715E-542.6470575
CCCTAAT16350.042.5688067
AAGACTA2400.042.2916684
TTGTGTT8400.042.2916642
CCTAATT16500.042.1818168
CTAATTA16850.041.3056379
Produced by FastQC (version 0.11.5)