FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_19_D.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_19_D.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379078
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG34290.9045631769714939No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA22690.5985575527991601No Hit
GACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAG22440.5919626040023426No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG21890.577453716649344No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG20460.5397306095315476No Hit
GTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAA17350.4576894464991374No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA17180.45320488131730147No Hit
GTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTTTAGTG14990.39543312985717977No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC14600.3851450097341444No Hit
ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAAT14440.3809242425041812No Hit
CGTTTATATAGCCCATCTGCCTTGTGTGGTCTGTATGAGTTTAGGCCTGA14140.37301030394800017No Hit
GTATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTTGCAA13790.3637773756324556No Hit
GCTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAATCTGCAGCAG12350.32579047056278654No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC11960.31550235043975117No Hit
GTGTTACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTA11540.30442283646109775No Hit
ATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCT11060.291760534771208No Hit
CCCTAATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAAC10940.28859495934873564No Hit
AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC10340.2727670822363735No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT10220.2696015068139011No Hit
AGTCAGCACTGCTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAA10020.26432554777644707No Hit
AAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAAC9980.26327035596895626No Hit
ATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGG9930.26195136620959275No Hit
GTTTTGTATGCGTCAATATGCTTATTCAGCAAAATGACTTGATCTTTGAA9750.2572030030758841No Hit
GTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCTCAG9600.2532460337977936No Hit
GCCTTGTGTGGTCTGTATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTC9540.2516632460865574No Hit
CCATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCA9390.24770627680846688No Hit
GTATACACTATGCGAGCAGAAGGGTAGTAGAGAGCTAGGCCAATAGCAAA8990.2371543587335588No Hit
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC8830.23293359150359555No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT8640.22792143041801424No Hit
ACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAA8590.22660244065865073No Hit
GTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCT8400.2215902795730694No Hit
CTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTC8090.21341254306501564No Hit
ACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCA7900.2084003819794343No Hit
GTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTT7860.20734519017194353No Hit
ATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGC7790.2054986045088346No Hit
CAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAA7670.20233302908636216No Hit
CATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGAC7530.19863985776014437No Hit
GTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGC7480.19732086800078086No Hit
GACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCC7230.1907259192039633No Hit
GTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCA7170.1891431314927271No Hit
GTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTTGCAATTGTTT7150.18861553558898167No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG7100.18729654582961816No Hit
GTTACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGA7080.18676894992587278No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA6970.18386717245527306No Hit
AGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTG6910.18228438474403685No Hit
ACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGAC6880.18149299088841875No Hit
GAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAA6860.18096539498467334No Hit
TTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAA6810.17964640522530984No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG6670.175953233899092No Hit
CCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACG6620.1746342441397285No Hit
GCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAA6530.17226006257287418No Hit
TCAATATGCTTATTCAGCAAAATGACTTGATCTTTGAAATTTGGATCTTT6430.16962208305414717No Hit
GATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGC6330.16698410353542015No Hit
CACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCA5950.15695978136425748No Hit
ATTCAAGGCTCCCTCAGTTGCAACCCATATGATGCCGTCTTTGTTAGCAC5890.15537699365302127No Hit
GAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAG5670.14957343871182185No Hit
GCACAAGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATAT5660.14930964075994915No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG5640.14878204485620372No Hit
CTCACATAGTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTATGC5590.1474630550968402No Hit
ATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACAT5540.1461440653374767No Hit
ACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGT5530.145880267385604No Hit
CTGTATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTTGC5510.14535267148185863No Hit
GCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACA5500.14508887352998592No Hit
GATTAGTTCCTGGTCCCCAAAATTTCCTTGGGTTTGTTCTGGACCACGTC5480.1445612776262405No Hit
TACTTGTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTAT5460.14403368172249512No Hit
CTCCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGG5440.1435060858187497No Hit
ATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTGCCTTG5410.1427146919631316No Hit
GTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTATATATC5410.1427146919631316No Hit
TCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATACAGACCACAC5380.14192329810751347No Hit
CTTCTGCGTAGAAGCCTTTTGGCAATGTTGTTCCTTGAGGAAGTTGTAGC5260.13875772268504108No Hit
GTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTG5240.13823012678129568No Hit
GTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCT5210.13743873292567757No Hit
CTGTATACACTATGCGAGCAGAAGGGTAGTAGAGAGCTAGGCCAATAGCA5160.13611974316631406No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG5160.13611974316631406No Hit
GCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTG5120.13506455135882325No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT5070.13374556159945974No Hit
GAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAAC5050.13321796569571434No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC5050.13321796569571434No Hit
GTTGCGACTACGTGATGAGGAACGAGAAGAGGCTTGACTGCCGCCTCTGC5040.13295416774384164No Hit
GGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCAT5010.13216277388822353No Hit
ACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTGCC4990.13163517798447813No Hit
GTGCTGACTCAACTCAGGCCTAAACTCATACAGACCACACAAGGCAGATG4970.13110758208073273No Hit
GTTCCTTGTCTGATTAGTTCCTGGTCCCCAAAATTTCCTTGGGTTTGTTC4950.13057998617698732No Hit
CTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACA4930.13005239027324192No Hit
GTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATT4920.12978859232136922No Hit
GGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCC4900.1292609964176238No Hit
AACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACC4890.1289971984657511No Hit
GTTTGTAATCAGTTCCTTGTCTGATTAGTTCCTGGTCCCCAAAATTTCCT4880.1287334005138784No Hit
GAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACA4880.1287334005138784No Hit
CACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATC4770.1258316230432787No Hit
GTCTGTATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTT4770.1258316230432787No Hit
TGCTTATTCAGCAAAATGACTTGATCTTTGAAATTTGGATCTTTGTCATC4760.125567825091406No Hit
GTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTATGCGAGCAGAA4740.12504022918766058No Hit
GGTTCAATCTGTCAAGCAGCAGCAAAGCAAGAGCAGCATCACCGCCATTG4710.12424883533204249No Hit
GTTCCCCTACTGCTGCCTGGAGTTGAATTTCTTGAACTGTTGCGACTACG4690.12372123942829708No Hit
TGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAA4630.12213845171706086No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG4600.12134705786144276No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA4580.12081946195769735No Hit
GGCTTGAGTTTCATCAGCCTTCTTCTTTTTGTCCTTTTTAGGCTCTGTTG4580.12081946195769735No Hit
CACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAA4550.12002806810207925No Hit
AGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTC4540.11976427015020655No Hit
GTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCACATGT4540.11976427015020655No Hit
GTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGA4450.11739008858335225No Hit
AGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAG4440.11712629063147953No Hit
GTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAA4430.11686249267960683No Hit
CACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTA4410.11633489677586144No Hit
ACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGAT4400.11607109882398874No Hit
GAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCG4390.11580730087211602No Hit
CCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGA4390.11580730087211602No Hit
TTCTTGAACTGTTGCGACTACGTGATGAGGAACGAGAAGAGGCTTGACTG4380.11554350292024332No Hit
CTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCTCAGCAGCA4360.11501590701649793No Hit
AACTCATACAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTT4340.11448831111275251No Hit
CTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGT4330.11422451316087981No Hit
GCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGC4290.113169321353389No Hit
TTTTTAGGCTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATT4230.11158653364215279No Hit
GTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGC4220.1113227356902801No Hit
GATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAA4210.1110589377384074No Hit
GTACACATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCAT4200.1107951397865347No Hit
CTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAATG4200.1107951397865347No Hit
GCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAAT4190.11053134183466201No Hit
ACATTTATCTATAGGCAAATATTTTAATGCCTTCTCACATAGTGCATCAA4170.1100037459309166No Hit
TTACCAGACATTTTGCTCTCAAGCTGGTTCAATCTGTCAAGCAGCAGCAA4160.10973994797904389No Hit
TTCTCACATAGTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTAT4150.10947615002717119No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC4150.10947615002717119No Hit
ATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTATATATCGTAAA4150.10947615002717119No Hit
AGTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATA4150.10947615002717119No Hit
CCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATT4140.1092123520752985No Hit
ACATCTACTTGTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAG4110.10842095821968038No Hit
GCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAAT4090.10789336231593499No Hit
CACCTGTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATG4090.10789336231593499No Hit
CCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTC4080.10762956436406226No Hit
ATATGATGCCGTCTTTGTTAGCACCATAGGGAAGTCCAGCTTCTGGCCCA4070.10736576641218958No Hit
CATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTG4060.10710196846031687No Hit
AAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTT4050.10683817050844417No Hit
CCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTC4040.10657437255657148No Hit
GTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTTGT4040.10657437255657148No Hit
GTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGT4020.10604677665282607No Hit
GTTCTAATGTTGAATTCACTTTGAATTTATCAAAACACTCTACACGAGCA4010.10578297870095336No Hit
ACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAA3980.10499158484533525No Hit
GCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCAC3970.10472778689346256No Hit
ACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGC3970.10472778689346256No Hit
ACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCT3960.10446398894158986No Hit
GACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCT3950.10420019098971715No Hit
TACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCA3950.10420019098971715No Hit
CTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAAT3940.10393639303784447No Hit
CACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTC3920.10340879713409905No Hit
ATATAGCCCATCTGCCTTGTGTGGTCTGTATGAGTTTAGGCCTGAGTTGA3900.10288120123035364No Hit
ATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAG3880.10235360532660825No Hit
TGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCT3870.10208980737473554No Hit
GTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTT3860.10182600942286284No Hit
AATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAG3840.10129841351911745No Hit
GTGCAGGTAATTGAGCAGGGTCGCCAATGTACACATAGTGCTTAGCACGT3820.10077081761537203No Hit
GTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGT3810.10050701966349933No Hit
ATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTA3800.10024322171162663No Hit
CTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTGCCT3800.10024322171162663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAAAG100.007089138145.05
TCGTGGG500.0145.0145
TCCCTAG1100.0112.045462
TTACCCA850.0110.882354
CACACCG850.0102.3529366
ACACCGC900.096.6666647
CCTAGCA3300.094.4696962
CCCTAGC3300.094.4696961
CTAGCAT3400.091.691183
GTCTGGA800.090.6252
TAGCATT3600.086.597224
TTTACCC1100.085.681823
GGACCTA850.085.294116
TACTGCG7200.081.56257
ACTGCGT7300.080.445218
ATACTGC7250.080.06
CTGCGTC7450.079.798669
ATTGTTC4150.075.120488
TAATACT8000.072.54
ATAATAC7900.072.53
Produced by FastQC (version 0.11.5)