FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_19_C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_19_C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582135
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGAAATCAAT44930.7718140981043916No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT33710.5790753004028275No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA27040.4644970668315769No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC26520.4555644309309696No Hit
TATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAA24330.41794429127264293No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT23060.39612804590000605No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC21080.3621153168938476No Hit
CATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACC20130.3457960782292767No Hit
CATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACA19540.33566097211128No Hit
GTAATAAGAACACCATTACGGGCATTTCTAAATAAGTCTACTTGACCATC19370.33274068729761996No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC18560.3188263890678279No Hit
TAATTGAACTGTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAG18000.3092066273287124No Hit
TTTCTACTCTGAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATC17540.30130468018586753No Hit
ACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGAC15750.2705557989126234No Hit
GTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAA14810.2544083417076795No Hit
GCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATC14770.25372121586917123No Hit
CTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACG14770.25372121586917123No Hit
GAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATT14720.2528623085710359No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA14520.2494266793784947No Hit
TCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCA14510.24925489791886762No Hit
ATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAG14450.24822420916110524No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT14090.242040076614531No Hit
GTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTG13960.2398069176393792No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG13240.22743865254623066No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT13150.22589261940958713No Hit
GTTTCAGGTAATTGTTGGACAACACCATCAACTTTCTTATAATAATTGAA12930.22211342729779177No Hit
GTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAAT12470.21421148015494687No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC12140.2085426919872538No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC12040.2068248773909832No Hit
GAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATCAACTTTCTTA11420.1961744268941053No Hit
GTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAA11230.19291057916119114No Hit
TTGTTGGACAACACCATCAACTTTCTTATAATAATTGAACTGTGTTTTTA10950.18810069829163337No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA10830.18603932077610866No Hit
CCATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAAC10660.18311903596244858No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC10610.18226012866431326No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA10220.17556065173885782No Hit
CCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCATTTCTAAA10210.17538887027923075No Hit
GTTGTAAACCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCA9880.1697200821115377No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC9860.16937651919228358No Hit
ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA9830.1688611748134024No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA9820.16868939335377534No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG9820.16868939335377534No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC9770.16783048605564No Hit
GTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGA9750.16748692313638588No Hit
TCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCA9540.1638795124842176No Hit
GTCTTAGGGTCGTACATATCACTAATAATGAGATCCCATTTATTAGCTGT9500.16319238664570934No Hit
GTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAGACTAGCTTGT9500.16319238664570934No Hit
GTAATAAATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCA9300.15975675745316809No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG9190.1578671613972704No Hit
GTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAAT9190.1578671613972704No Hit
TATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACT9150.15718003555876214No Hit
ATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGACAA9130.156836472639508No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT9070.15580578388174565No Hit
AGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAACACTACC9070.15580578388174565No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC9060.15563400242211858No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG8920.1532290619873397No Hit
GTATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGAC8830.15168302885069615No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG8810.15133946593144204No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT8810.15133946593144204No Hit
ACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATG8800.15116768447181494No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG8610.14790383673890076No Hit
ATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTG8540.14670136652151133No Hit
GTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAA8480.14567067776374898No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT8450.14515533338486777No Hit
TTCAAAAGGTGATTCCTTAAAACGTTTAGCTAGTCCAATCAGTAGATGTA8330.14309395586934304No Hit
GAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTTTAGCTAGT8310.1427503929500889No Hit
AGCTTATAAAGATCAGCATTCCAAGAATGTTCTGTTATCTTTATAGCCAC8280.1422350485712077No Hit
TTTCTAAATAAGTCTACTTGACCATCAACTCTACCATCAAAAAAGACAGT8180.1405172339749371No Hit
ATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCA7930.13622269748426052No Hit
CTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCC7880.1353637901861252No Hit
CCTTAAAACGTTTAGCTAGTCCAATCAGTAGATGTAAACCACCTAACTGA7840.13467666434761696No Hit
TTCCAAGAATGTTCTGTTATCTTTATAGCCACGGAACCTCCAAGAGCTAG7720.1326152868320922No Hit
GTTATGAAATAGTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAA7700.1322717239128381No Hit
GTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGC7570.13003856493768626No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT7370.12660293574514503No Hit
TATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATT7320.12574402844700971No Hit
TTTATTAGCTGTATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTG7310.12557224698738267No Hit
CAGTAGATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGA7280.12505690260850147No Hit
GTTCGAAGGCATAGCCTTCTAATTTATACCGTTCAATGAATTCATCCATA7240.12436977676999321No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAATAACTCGTC7190.12351086947185791No Hit
AGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTC7150.12282374363334965No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC7100.12196483633521434No Hit
ACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGC7090.12179305487558728No Hit
CCCTTACGAAGAATGGTTTTCAAGCCAGATTCATTATGGTATTCGGCAAG7080.12162127341596021No Hit
AACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTT7050.12110592903707904No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA7050.12110592903707904No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT7010.12041880319857079No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG7000.12024702173894372No Hit
GCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAA6960.11955989590043546No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC6940.11921633298118135No Hit
AGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTCTAATA6920.11887277006192722No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG6870.11801386276379192No Hit
ATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAACAC6850.11767029984453778No Hit
GTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTAT6780.11646782962714834No Hit
ATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGATATGTT6700.11509357795013184No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA6690.11492179649050477No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC6670.11457823357125066No Hit
CTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTA6650.11423467065199654No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG6640.11406288919236947No Hit
AATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATG6600.11337576335386122No Hit
GTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGAT6530.11217329313647179No Hit
CATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAA6480.11131438583833646No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT6440.11062725999982821No Hit
CTTTATAGCCACGGAACCTCCAAGAGCTAGCTTTTGTTGTATAAACCCAC6380.10959657124206584No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC6350.10908122686318467No Hit
GCCTTCTAATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGA6340.1089094454035576No Hit
ATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAG6290.10805053810542227No Hit
CTTTAGAGTCATTTTCTTTTGTAACATTTTTAGTCTTAGGGTCGTACATA6250.10736341226691402No Hit
GAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTT6230.1070198493476599No Hit
CTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCAT6200.10650450496877872No Hit
CCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTC6110.10495847183213515No Hit
CATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAACA6080.10444312745325397No Hit
ACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATG6080.10444312745325397No Hit
TTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATTTGTGCACCAC6010.10324065723586454No Hit
GTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA5990.10289709431661041No Hit
CAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACA5980.10272531285698334No Hit
GTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAA5970.10255353139735628No Hit
GATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGATATGT5940.1020381870184751No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC5920.10169462409922096No Hit
ACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAAT5910.10152284263959391No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAAC100.0070901075144.999981
CCTTACG850.0119.4117662
CTCCTAG1550.098.225811
TCCTAGC1700.089.558832
CCTAGCA1850.086.216223
TAGCACC1900.083.9473655
CCCTTAC1250.081.21
ATAGGGA1600.067.968741
TAGGGAA1600.067.968742
TTGTAGA4700.067.8723452
GGGAAGT1650.065.909094
TCTGTTC4900.065.102049
AGGGAAG1700.063.970593
AAACCTC851.3040335E-859.7058835
GGAAGTC1850.058.7837875
CAAACCT753.7584505E-758.04
ACTTTCG753.7584505E-758.01
GAAGTCC1900.057.236846
GAAGAAT1800.056.388898
GCTGTCC1750.053.857141
Produced by FastQC (version 0.11.5)