FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_03_C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_03_C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521536
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGAAATCAAT36280.6956375015339306No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA30550.585769726346791No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC23420.4490581666462143No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT23060.4421554791999018No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT22640.43410234384587065No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC16930.3246180512946374No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG16380.3140722788072156No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG16060.3079365566327157No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC15140.2902963553810283No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC15020.28799545956559086No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC14270.2736148607191066No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA14240.2730396367652473No Hit
TATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAA14020.26882132777027856No Hit
CATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACA12450.23871794085163822No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT12350.236800527672107No Hit
GAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATT12300.2358418210823414No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT11890.22798042704626334No Hit
GTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAAT11710.22452908332310714No Hit
CATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACC11620.22280341146152902No Hit
GCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATC11460.21973555037427903No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT11380.21820161983065406No Hit
CTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACG10890.20880629525095104No Hit
GTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAA10820.20746410602527918No Hit
TTTCTACTCTGAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATC10670.20458798625598235No Hit
ATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGACAA10500.20132838385077925No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC10470.20075315989691989No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA10270.1969183335378574No Hit
GTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAA10220.19595962694809177No Hit
TCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCA10210.19576788563013867No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT10150.19461743772241993No Hit
GTATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGAC10080.19327524849674807No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG9970.1911660939992637No Hit
ACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGAC9640.18483863050681065No Hit
GTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAAT9540.18292121732727942No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT9500.18215425205546693No Hit
GTCTTAGGGTCGTACATATCACTAATAATGAGATCCCATTTATTAGCTGT9350.17927813228617007No Hit
ATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAG9340.17908639096821696No Hit
TCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCA9020.172950668793717No Hit
GTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGA8980.17218370352190454No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA8980.17218370352190454No Hit
AGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTCTAATA8960.17180022088599828No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT8920.1710332556141858No Hit
GTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAG8870.17007454902442018No Hit
GTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGA8810.16892410111670142No Hit
GTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTG8530.163555344214014No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC8470.16240489630629523No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG8440.1618296723524359No Hit
TAATTGAACTGTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAG8390.16087096576267026No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC8120.15569395017793594No Hit
ATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCA7920.15185912381887348No Hit
TTTATTAGCTGTATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTG7840.1503251932752485No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC7710.1478325561418579No Hit
ACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATG7680.14725733218799852No Hit
TTGTTGGACAACACCATCAACTTTCTTATAATAATTGAACTGTGTTTTTA7590.1455316603264204No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC7460.14303902319302983No Hit
GAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATCAACTTTCTTA7440.1426555405571236No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG7430.14246379923917044No Hit
CCCTTACGAAGAATGGTTTTCAAGCCAGATTCATTATGGTATTCGGCAAG7390.14169683396735797No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG7360.1411216100134986No Hit
CTTTATAGCCACGGAACCTCCAAGAGCTAGCTTTTGTTGTATAAACCCAC7330.1405463860596392No Hit
CCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTC7320.1403546447416861No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC7260.13920419683396734No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC7250.13901245551601424No Hit
GTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGT7250.13901245551601424No Hit
TTCCAAGAATGTTCTGTTATCTTTATAGCCACGGAACCTCCAAGAGCTAG7230.13862897288010798No Hit
ATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTCTAATAGCATT7120.13651981838262364No Hit
GGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCAT6970.13364369861332678No Hit
ATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTAT6940.13306847465946742No Hit
CTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCC6930.13287673334151429No Hit
GTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA6810.13057583752607682No Hit
CCATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAAC6580.126165787213155No Hit
CTTTAGAGTCATTTTCTTTTGTAACATTTTTAGTCTTAGGGTCGTACATA6530.12520708062338937No Hit
AGCTTATAAAGATCAGCATTCCAAGAATGTTCTGTTATCTTTATAGCCAC6510.12482359798748313No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC6460.12386489139771752No Hit
GTAATAAGAACACCATTACGGGCATTTCTAAATAAGTCTACTTGACCATC6460.12386489139771752No Hit
CCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCATTTCTAAA6440.12348140876181127No Hit
AACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTT6370.12213921953613942No Hit
CTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTA6350.12175573690023314No Hit
GTTGTAAACCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCA6280.12041354767456129No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG6230.11945484108479568No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG6210.11907135844888943No Hit
CAGTAGATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGA6160.11811265185912381No Hit
CATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTT6150.1179209105411707No Hit
AGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTC6150.1179209105411707No Hit
GTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAGACTAGCTTGT6130.11753742790526446No Hit
GAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACA6010.11523653208982698No Hit
GATTAGGCATAGCAACACCCGGTTGCCACGCTTGACTAGATTGTAATTTT6010.11523653208982698No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT5940.11389434286415512No Hit
CCATAATTTTGAAGGTCACACTTTTCTAATAGCATTCTTTGCATTTTGTA5890.1129356362743895No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT5850.11216867100257699No Hit
GTTCGAAGGCATAGCCTTCTAATTTATACCGTTCAATGAATTCATCCATA5820.11159344704871764No Hit
GTAATAAATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCA5800.1112099644128114No Hit
ACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAAT5790.11101822309485826No Hit
GTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCATCTTGGACTG5770.11063474045895202No Hit
GTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACA5760.1104429991409989No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT5750.11025125782304578No Hit
GTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGACAAAGTCATT5690.10910080991532703No Hit
ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA5680.10890906859737393No Hit
ACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGC5590.1071833967357958No Hit
GAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTTTAGCTAGT5590.1071833967357958No Hit
ATGTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAAT5510.10564946619217083No Hit
AGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAA5480.10507424223831145No Hit
CCTTAGAAACTACAGATAAATCTTGGGATTTTATTATTTCAACAAAATCA5430.10411553564854584No Hit
ATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCATC5430.10411553564854584No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC5430.10411553564854584No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT5420.1039237943305927No Hit
GTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAA5420.1039237943305927No Hit
GTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGC5390.10334857037673335No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC5380.10315682905878021No Hit
CTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTTG5380.10315682905878021No Hit
GTATTTTGAGAGTATAAGTTGGATATGTGATGTGAGAGAAAGAGAGGAAT5280.10123941587924898No Hit
GCCTTCTAATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGA5270.10104767456129587No Hit
ACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTG5260.10085593324334274No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACT5260.10085593324334274No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG5250.10066419192538963No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCC151.2296703E-4145.05
AGCGGCC100.007089896145.09
ACTTTCG351.0913936E-11145.01
CTCCTAG1200.0108.751
TAGCACC1300.0100.384625
GGGCTTT900.096.6666641
ATTTGAG1000.094.252
GCAAACC700.093.214293
TCCTAGC1450.090.02
GGCTTTT750.087.02
CTACCCC259.384212E-487.04
CCTAGCA1550.084.193553
CCCCCTT353.4357312E-582.857148
TACCTGC451.2320561E-680.555566
ATAGGGA2300.078.8043441
AGCTTTG1200.078.5416647
TAGGGAA2400.075.5208362
CCCTTAC1550.074.8387151
CCCCTTT406.6602E-572.59
ATAGGAG300.001935220972.55
Produced by FastQC (version 0.11.5)