FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_02_D.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_02_D.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502906
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG73741.466277992308702No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG35940.7146464746891069No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA34540.6868082703328257No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG33330.6627481079963253No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA31750.6313307059370936No Hit
GACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAG30900.6144289390064943No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC27830.553383733739506No Hit
GTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAA26330.5235570862149189No Hit
ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAAT23400.46529570138355875No Hit
GTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTTTAGTG22300.44342282653219484No Hit
AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC22270.4428262935817031No Hit
CGTTTATATAGCCCATCTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGA21080.41916381987886403No Hit
GTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCTCAG20530.40822738245318213No Hit
GCTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAATCTGCAGCAG19630.39033139393842986No Hit
AAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAAC19310.38396837579985127No Hit
CCATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCA17950.3569255487108923No Hit
ATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCT17750.3529486623742807No Hit
GTGTTACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTA17550.34897177603766905No Hit
CCCTAATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAAC17450.3469833328693632No Hit
ATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGG17350.34499488970105746No Hit
GCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTC16490.3278942784536275No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA16080.3197416614635737No Hit
GTTTTGTATGCGTCAATATGCTTATTCAGCAAAATGACTTGATCTTTGAA15510.3084075354042306No Hit
ACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAA14680.29190345710729243No Hit
GTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCT14630.2909092355231395No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG14530.2889207923548337No Hit
CAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAA14420.2867335048696973No Hit
ACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGAC14110.2805693310479493No Hit
AGTCAGCACTGCTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAA14000.27838204356281293No Hit
GAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAA13460.26764445045396157No Hit
GTTACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGA13300.2644629413846723No Hit
GTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTTGCAATTGTTT13290.2642640970678417No Hit
TCAATATGCTTATTCAGCAAAATGACTTGATCTTTGAAATTTGGATCTTT13190.2622756538995359No Hit
GACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCC12970.2579010789292631No Hit
AGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTG12610.25074268352336226No Hit
ATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGC12570.2499473062560399No Hit
ATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTGCCTTG12530.24915192898871757No Hit
TACTTGTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTAT12270.2439819767511225No Hit
GATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGC12040.23940855746401912No Hit
GTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTT12030.23920971314718856No Hit
CCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACG11880.2362270483947298No Hit
CTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTC11470.22807443140467604No Hit
GTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTATATATC11460.22787558708784544No Hit
ACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCA10990.21852990419680818No Hit
ATTCAAGGCTCCCTCAGTTGCAACCCATATGATGCCGTCTTTGTTAGCAC10770.21415532922653538No Hit
ACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTGCC10570.21017844288992374No Hit
GTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTG10530.20938306562260145No Hit
GTTGCGACTACGTGATGAGGAACGAGAAGAGGCTTGACTGCCGCCTCTGC10390.2065992451869733No Hit
GCACAAGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATAT10260.20401426906817574No Hit
CTCCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGG10230.203417736117684No Hit
TCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACAC10150.20182698158303938No Hit
TGCTTATTCAGCAAAATGACTTGATCTTTGAAATTTGGATCTTTGTCATC9890.1966570293454443No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC9730.193475520276155No Hit
ACATCTACTTGTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAG9500.18890210098905164No Hit
ATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTATATATCGTAAA9450.18790787940489873No Hit
CTTCTGCGTAGAAGCCTTTTGGCAATGTTGTTCCTTGAGGAAGTTGTAGC9340.18572059191976234No Hit
GTTCCTTGTCTGATTAGTTCCTGGTCCCCAAAATTTCCTTGGGTTTGTTC9250.1839309930682871No Hit
GCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACA9230.18353330443462595No Hit
ATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACAT9110.18114717263265898No Hit
TTCTTGAACTGTTGCGACTACGTGATGAGGAACGAGAAGAGGCTTGACTG9010.17915872946435318No Hit
CACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCA8840.17577837607823332No Hit
ATATAGCCCATCTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGA8750.1739887772267581No Hit
GAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAG8720.17339224427626634No Hit
GTTTGTAATCAGTTCCTTGTCTGATTAGTTCCTGGTCCCCAAAATTTCCT8670.17239802269211343No Hit
CTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCTCAGCAGCA8640.1718014897416217No Hit
TTACCAGACATTTTGCTCTCAAGCTGGTTCAATCTGTCAAGCAGCAGCAA8580.1706084238406382No Hit
ACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGC8580.1706084238406382No Hit
GGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCAT8560.17021073520697705No Hit
AGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTC8550.17001189089014648No Hit
CACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTC8420.1674269147713489No Hit
GTTCCCCTACTGCTGCCTGGAGTTGAATTTCTTGAACTGTTGCGACTACG8400.16702922613768778No Hit
GATTAGTTCCTGGTCCCCAAAATTTCCTTGGGTTTGTTCTGGACCACGTC8350.16603500455353487No Hit
AACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACC8320.16543847160304312No Hit
GAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAAC8210.16325118411790673No Hit
TTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAA8170.16245580685058442No Hit
TTTTTAGGCTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATT8100.16106389663277035No Hit
GCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAA8060.16026851936544803No Hit
GGTTCAATCTGTCAAGCAGCAGCAAAGCAAGAGCAGCATCACCGCCATTG8040.15987083073178684No Hit
GAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACA7980.15867776483080337No Hit
TCTTAGAAGCCTCAGCAGCAGATTTCTTAGTGACAGTTTGGCCTTGTTGT7980.15867776483080337No Hit
ATATGATGCCGTCTTTGTTAGCACCATAGGGAAGTCCAGCTTCTGGCCCA7940.15788238756348105No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC7890.15688816597932814No Hit
CCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGA7830.15569510007834467No Hit
ACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGAT7700.1531101239595471No Hit
CACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATC7680.15271243532588596No Hit
GTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGA7670.15251359100905537No Hit
TGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAA7610.15132052510807187No Hit
CACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAA7380.14674710582096853No Hit
CTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGT7370.14654826150413797No Hit
CCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATT7360.14634941718730737No Hit
GCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGC7360.14634941718730737No Hit
CTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAATG7340.14595172855364622No Hit
CCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTC7230.14376444106850983No Hit
ATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAG7150.14217368653386517No Hit
GTCAAGCAGCAGCAAAGCAAGAGCAGCATCACCGCCATTGCCAGCCATTC7140.1419748422170346No Hit
GCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTTGCAA6950.13819680019725356No Hit
GCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTT6910.13740142292993124No Hit
GTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATG6850.13620835702894776No Hit
AATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAG6830.13581066839528658No Hit
CATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTG6770.1346176024943031No Hit
GTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAATGAC6750.13421991386064194No Hit
GGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCC6710.13342453659331963No Hit
GTTGCAATTGTTTGGAGAAATCATCCAAATCTGCAGCAGGAAGAAGAGTC6700.13322569227648906No Hit
CTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTGCCT6690.13302684795965847No Hit
GTTAGCAGGATTGCGGGTGCCAATGTGATCTTTTGGTGTATTCAAGGCTC6680.13282800364282787No Hit
GCTCCCTTCTGCGTAGAAGCCTTTTGGCAATGTTGTTCCTTGAGGAAGTT6650.13223147069233615No Hit
GGCTTGAGTTTCATCAGCCTTCTTCTTTTTGTCCTTTTTAGGCTCTGTTG6650.13223147069233615No Hit
GTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCT6590.13103840479135265No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT6530.12984533889036917No Hit
GAGTTGAGTCAGCACTGCTCATGGATTGTTGCAATTGTTTGGAGAAATCA6340.12606729687058812No Hit
ATTCCGAAGAACGCTGAAGCGCTGGGGGCAAATTGTGCAATTTGCGGCCA6340.12606729687058812No Hit
CTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAG6280.12487423096960465No Hit
GCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCA6240.12407885370228233No Hit
TTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCTC6210.1234823207517906No Hit
GATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAA6190.12308463211812944No Hit
ATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGG6110.12149387758348477No Hit
GCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAAT6070.12069850031616247No Hit
ATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTT6040.12010196736567072No Hit
ATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAG6030.11990312304884015No Hit
ATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAA6030.11990312304884015No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATATCAGATGAGTTT5940.1181135241973649No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG5930.11791467988053433No Hit
GTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGT5910.11751699124687316No Hit
GATATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTG5870.11672161397955085No Hit
CTTAGTGACAGTTTGGCCTTGTTGTTGTTGGCCTTTACCAGACATTTTGC5860.11652276966272028No Hit
TCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATT5810.11552854807856737No Hit
ATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCT5800.11532970376173679No Hit
CCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTC5790.1151308594449062No Hit
GCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCAC5760.11453432649441446No Hit
GTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGT5700.11334126059343097No Hit
GTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGAT5680.11294357195976981No Hit
GGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAAT5640.1121481946924475No Hit
GGTTTGTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTT5630.11194935037561692No Hit
GTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTT5620.11175050605878632No Hit
CTTAGAAGCCTCAGCAGCAGATTTCTTAGTGACAGTTTGGCCTTGTTGTT5600.11135281742512518No Hit
GTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTG5580.110955128791464No Hit
TTCCATGCCAATGCGCGACATTCCGAAGAACGCTGAAGCGCTGGGGGCAA5560.11055744015780285No Hit
CACCTGTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATG5550.11035859584097227No Hit
TCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGG5550.11035859584097227No Hit
TGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTT5510.10956321857364995No Hit
ACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCT5500.10936437425681936No Hit
GTATACACTATGCGAGCAGAAGGGTAGTAGAGAGCTAGGCCAATAGCAAA5500.10936437425681936No Hit
CCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAA5470.10876784130632762No Hit
GCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAAT5470.10876784130632762No Hit
GCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTATATATCGT5440.10817130835583588No Hit
TATATAGCCCATCTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTG5440.10817130835583588No Hit
GCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCTCAGCAGC5430.1079724640390053No Hit
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC5430.1079724640390053No Hit
GGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGC5420.10777361972217471No Hit
TTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGT5410.10757477540534413No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG5340.10618286518753008No Hit
GAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGT5340.10618286518753008No Hit
AATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTG5330.10598402087069948No Hit
TCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGG5250.10439326633605486No Hit
GTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAAT5230.10399557770239369No Hit
CATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTC5200.10339904475190195No Hit
GAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGC5200.10339904475190195No Hit
GCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATT5060.10061522431627382No Hit
GGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGACATTC5030.10001869136578208No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGG100.007089821145.0145
TCGTGGG1250.0127.6145
TCCCTAG1350.0118.148152
CCCTAGC5750.0109.6956561
TTACCCA1800.0108.749994
TTTACCC1850.0105.8108143
CCTAGCA6000.0105.1252
CTAGCAT6000.0105.1253
TAGCATT6050.0104.2561954
ATTGTTC7400.086.216228
ATAATAC15100.079.701993
TAATACT15700.077.579624
TACTGCG15950.075.909097
ACTGCGT15800.075.712038
ATACTGC16150.075.417956
CTGCGTC16050.074.984439
AATAATA16550.073.595162
CAATAAT16600.072.9367451
TTGTTCA8650.072.9190759
AAAACGG300.001935180672.5145
Produced by FastQC (version 0.11.5)