FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n02_CV_02_C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n02_CV_02_C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198490
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGCACCATGGCACAGAGAGGTTAAGTAACATGCCCGAAGCTACAC5450.27457302634893443No Hit
GTGTAGGACTGGGGCACCAAGAGAAAGGAGCAGAGAGAGGGTATCTGAAT4010.2020252909466472No Hit
AATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGAAATCAAT3490.17582749760693234No Hit
GTCCTACACACTGCCCCGGGACCCTCCCAAATGTCCCACACAGGCCTGGG3370.16978185299007506No Hit
CCCTTATCCTCAGTCTGACTAACTTCTGCTCATCTTCCACACCTCCACCA3030.15265252657564612No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT2160.10882160310343089No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA2140.10781399566728803No Hit
CTATAACATTCACCCTGCCTTCAACCCATGGTTATTTGGGACTTAACTGA2110.10630258451307371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACC1251.8189894E-1258.05
CCTAGCA1700.046.9117623
TCCTAGC1751.8189894E-1245.5714262
CTAGCAC1801.8189894E-1244.3055574
GTACTAA750.001480519938.6666641
ATACACC1157.1138384E-637.8260883
ATAGGGA2002.2191671E-1036.251
CTCCTAC850.002736717734.1176451
CTCCTAG3850.032.012991
GGAAGTC2351.2496457E-930.8510655
TAAGATA950.00471826730.5263184
GAAGTCC2151.7949787E-830.3488376
TATACCG1555.4785552E-528.0645145
ATACCGT1555.4785552E-528.0645146
GCACCAT2354.2611646E-827.7659597
TACTGCT1050.00769381527.6190477
TATACCA1050.00769381527.6190474
CCGTTCA1606.801047E-527.18759
TAGGGAA2456.383925E-826.6326522
AGCACCA2507.76472E-826.16
Produced by FastQC (version 0.11.5)