FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717259
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG492176.861817000553495No Hit
ATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGC28330.3949758734292634No Hit
GACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAG23170.32303533312234495No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.3029589032692514No Hit
TCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACAC19600.27326251744488395No Hit
GTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATG18810.262248364956034No Hit
GGCCTAAACTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTT13440.18738001196220613No Hit
ATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGG12820.1787359935532353No Hit
ACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATA11260.15698652787905068No Hit
ACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAA10220.1424868840962609No Hit
GTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAA9860.13746777663298754No Hit
GAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAA9200.12826607961698633No Hit
ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAAT8890.12394407041250093No Hit
GGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGG8640.12045857911856107No Hit
CAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCA8630.12031915946680348No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGA8610.12004032016328829No Hit
ATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAG7960.1109780427990447No Hit
GTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGAT7670.10693487289807448No Hit
ACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCT7300.10177634578304351No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG7250.10107924752425554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCGA151.2298614E-4145.06
ATAATCG259.3856576E-487.03
ACTCGAC259.3856576E-487.07
GCATCCT451.193629E-464.44444145
ATAGAGC350.003566092362.1428573
CTCGACT450.00963803448.3333328
CCTATAC604.94414E-448.3333323
TAGTATT450.00963803448.3333324
ATGTCCT657.33519E-444.6153874
GCTTAAC2300.044.1304361
ATTATAT855.339977E-542.647062
CCGTGAC700.00105644141.428573
GTAGATC907.480322E-540.277784
CCTAGTA800.002036231836.252
TTGAAAG13600.034.650738145
TAATACT3150.034.523814
TTATAGT1051.8531948E-434.5238084
CTTAACC3050.033.2786872
TCCATAG5500.032.954544145
CTAATTA3300.032.9545449
Produced by FastQC (version 0.11.5)