FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_36_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_36_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515149
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTTTTGG67991.3198123261425336No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG46160.896051433662882No Hit
CCCAAAAATGGATCATTACAAAATTGCCTGTTAGGCAGCTCTCCCTAGCA41550.8065627614534825No Hit
CCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGAC40690.7898685622994512No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT34850.6765033029278907No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGCCTGTTAGGCAGCTCTC31820.6176853686991531No Hit
CCCTAGCATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTG31050.6027382368984507No Hit
GTGCTATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGATCCAT27070.525479036162353No Hit
GTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA26660.5175201737749661No Hit
GCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTTTTGGGT26000.5047083465172212No Hit
CCCAAAAATGGATCATTACAAAATTGACACCCAAAAAGTCACTGGGCCTC25700.4988847886727918No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT25600.4969436027246486No Hit
ATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGT24060.4670493391232439No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT23810.462196374252886No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC23260.4515198515380987No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA20140.39095484995603214No Hit
CACCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCG19650.38144303881013064No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGACACCCAAAAAGTCACT18570.3604782305701845No Hit
GCCTAACAGGCAATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTC17950.34844287769169696No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC17820.3459193359591109No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT17120.33233103432210875No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT16930.32864278102063677No Hit
CATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTGCCTGTT16660.32340157896065025No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA15900.30864856575476224No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG15120.29350731535924557No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG14750.2863249273511159No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT13900.26982484679189905No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT13870.2692424910074561No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC13740.26671894927487No Hit
ATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA13390.25992479845636896No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC12890.25021886871565313No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC12610.24478354806085229No Hit
TGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC12360.23993058319049437No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT12200.23682468567346535No Hit
CTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC12160.23604821129420808No Hit
GTCCAATGTGCTATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAAT12090.23468938113050788No Hit
ACATTGGACACATTGAGCCCACAATACACCCAAAAATGGATCATTACAAA12070.23430114394087923No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG11950.23197172080310743No Hit
GCCCACAATACACCCAAAAATGGATCATTACAAAATTGCCTGTTAGGCAG11720.22750699312237818No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT11310.21954813073499124No Hit
ATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTCAATGTGTCCAGC11140.21624811462314786No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA10890.21139514975278997No Hit
CCCAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTT10560.20498923612391756No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC10490.20363040596021736No Hit
CAATCAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCAT9800.19023622291802955No Hit
TTACAAAATTGATGGATCATTACAAAATTGCTGGACACATTGAGCCCACA9750.18926562994395796No Hit
TTACAAAATTGCCTGTTAGGCAGCTCTCCCTAGCATTGTAGGCAGCTCTC9720.188683274159515No Hit
ATGCTAGGGAGAGCTGCCTACAATGCTAGGGAGAGCTGCCTAACAGGCAA9700.18829503696988636No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC9690.18810091837507206No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA9510.1846067836684144No Hit
CATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTGACACCC9450.18344207209952848No Hit
CTATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGATCCATTTT9230.17917146301361353No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA9030.1752890911173272No Hit
CAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTTTT8930.17334790516918408No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT8880.1723773121951125No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC8850.17179495641066952No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA8730.16946553327289773No Hit
CCCAAAAATGGATCATTACAAAATTGATGGATCATTACAAAATTGCTGGA8720.16927141467808343No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC8600.16694199154031164No Hit
CCACAATACACCCAAAAATGGATCATTACAAAATTGCCTGTTAGGCAGCT8550.16597139856624005No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC8480.16461256840253985No Hit
GGTTCACAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCC8400.16305961964402532No Hit
CCAATGTGCTATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGA8380.1626713824543967No Hit
TGATAGAGCCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTG8280.16073019650625353No Hit
GGACACATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTGC8260.1603419593166249No Hit
TTACAAAATTGCTGGACACATTGAGCCCACAATACACCCAAAAATGGATC8250.16014784072181057No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGATGGATCATTACAAAAT8210.1593713663425533No Hit
ATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCG8090.15704194320478151No Hit
GGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGAT8070.15665370601515288No Hit
TCATAGCACATTGGACACATTGAGCCCACAATACACCCAAAAATGGATCA8020.15568311304108132No Hit
GCCTACAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCA8000.15529487585145269No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT7950.15432428287738112No Hit
AGCCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTT7950.15432428287738112No Hit
TTACAAAATTGCCTGTTAGGCAGCTCTCCCTAGCATTGAGCCCACAATAC7880.1529654527136809No Hit
TTACAAAATTGACACCCAAAAAGTCACTGGGCCTCATAGCACATTGGACA7830.15199485973960933No Hit
GCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTT7640.1483066064381373No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC7630.14811248784332298No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC7570.1469477762744371No Hit
ATTTTGTAATGATCCATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGG7450.14461835313666532No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA7430.1442301159470367No Hit
CCCTAGCATTGGTGCCTGGACACATTGAGCCCACAATACACCCAAAAATG7430.1442301159470367No Hit
ATTTTTGGGTGTATTGTGGGCTCAATGTGTCCAGCAATTTTGTAATGATC7160.13898891388705015No Hit
GGCTCAATGTGTCCAATGTGCTATGAGGCCCAGTGACTTTTTGGGTGTCA7130.1384065581026072No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT6850.13297123744780637No Hit
GGACACATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTGA6840.13277711885299207No Hit
CTCCCTAGCATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAAT6620.1285065097670771No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA6510.12637120522411963No Hit
GTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAA6510.12637120522411963No Hit
AGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTTTTGGGTGTA6480.12578884943967666No Hit
GCCCACAATACACCCAAAAATGGATCATTACAAAATTGACACCCAAAAAG6480.12578884943967666No Hit
GATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC6480.12578884943967666No Hit
GTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCT6380.12384766349153352No Hit
CCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG6310.12248883332783331No Hit
CAATGTGTCCAATGTGCTATGAGGCCCAGTGACTTTTTGGGTGTCAATTT6300.122294714733019No Hit
CAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCT6190.12015941019006152No Hit
CCAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATTTT6160.11957705440561855No Hit
GGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCT6110.11860646143154699No Hit
GCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGATCCATTTTTGGGTGT6080.11802410564710404No Hit
CCACCAATGTTCTATGAGGCCCAACACCCAAAAATGGATCATTACAAAAT6020.11685939407821815No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG5990.11627703829377521No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT5980.11608291969896088No Hit
GAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAAT5920.11491820813007499No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT5910.11472408953526066No Hit
ATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGATCCATTTTTG5860.11375349656118909No Hit
CTACAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATT5860.11375349656118909No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT5830.11317114077674614No Hit
ATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTCAATGTGTCCAAT5830.11317114077674614No Hit
ACAATACACCCAAAAATGGATCATTACAAAATTGCCTGTTAGGCAGCTCT5830.11317114077674614No Hit
CACCAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATT5810.11278290358711751No Hit
CCAATATTCCAGGCACCAATGCTAGGGAGAGCTGCCTAACAGGCAATTTT5790.11239466639748888No Hit
GGTTCAAACAGATGCGCAAACAGGTTCACAATGCTAGGGAGAGCTGCCTA5770.11200642920786025No Hit
CAATAATGCTAGGGAGAGCTGCCTAACAGGCAATTTTGTAATGATCCATT5690.11045348044934572No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTA5690.11045348044934572No Hit
GCATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTGCCTGT5650.10967700607008846No Hit
ATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTCAATGTGTCCAGG5620.1090946502856455No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTAACAGGCAATTT5570.10812405731157393No Hit
GCTATGAGGCCCAGTGACTTTTTGGGTGTCAATTTTGTAATGATCCATTT5530.10734758293231668No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT5500.10676522714787372No Hit
TTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTA5500.10676522714787372No Hit
ATTGATGGATCATTACAAAATTGCTGGACACATTGAGCCCACAATACACC5490.10657110855305942No Hit
AGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGA5470.10618287136343078No Hit
ATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTT5430.10540639698417352No Hit
ATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTCAATGCTAGGGAG5270.10230049946714445No Hit
CATTGGACACATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAA5230.10152402508788719No Hit
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT5200.10094166930344425No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG100.0070898705145.05
TAATACC100.0070898705145.04
ACTATAC100.0070898705145.05
GATACGC100.0070898705145.08
CAACGCT253.7089194E-8145.04
CCGATGT253.7089194E-8145.09
CTATCGA151.2296617E-4145.04
GGCCCGC100.0070898705145.06
ATCCGTT100.0070898705145.07
TATTGCC306.493792E-10145.07
GTATGTA100.0070898705145.02
AACGCTA306.493792E-10145.05
CTACCGG100.0070898705145.05
ACGCTAG306.493792E-10145.06
CGTAAAG151.2296617E-4145.01
CCGAAAA253.7089194E-8145.09
GGACCGA100.0070898705145.06
ACCCGAA253.7089194E-8145.07
GCTACGA202.1296164E-6145.03
CCTATCG151.2296617E-4145.03
Produced by FastQC (version 0.11.5)