FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_36_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_36_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587471
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAGAAATTAACAT107321.8268135788830426No Hit
ATTATGAACAGCTCTCTTTTATTATGGATGCATTTGGCCACTGCTGTAAT70641.2024423333236876No Hit
CTTTTATTATGGATGCATTTGGCCACTGCTGTAATTTTTTAAGAAACATG69271.1791220332578118No Hit
GTTTACAGTTGCTGAAGTTTGATTGATCTGTTGATAAACACTAATGTTTT52960.9014913076560376No Hit
CATTTGGCCACTGCTGTAATTTTTTAAGAAACATGTTCTCATCTCAACCA47640.8109336460863601No Hit
CCTATAAATAGTATCAGTGTCATCATCCTTTTTAAGTTAATAATGAGCCA47550.8094016555710836No Hit
GCTTAACCCTAATTATGAACAGCTCTCTTTTATTATGGATGCATTTGGCC46140.7854004708317517No Hit
CCCTAATTATGAACAGCTCTCTTTTATTATGGATGCATTTGGCCACTGCT38760.6597772485790788No Hit
CCCCAGAAAGGTGGGCAAGTGGCTGTTATTGTTAGTAGGTTTCACTTAGC38060.647861766793595No Hit
GTTTATTTGGTTTGGATATGGGGACATAGGACTCCCCAGAAAGGTGGGCA37900.6451382280997701No Hit
TGCTTAACCCTAATTATGAACAGCTCTCTTTTATTATGGATGCATTTGGC30320.516110582479816No Hit
GTTGATAAACACTAATGTTTTCCAGTTGACAATTTCTCCATGTTAATTTC29360.49976935031686676No Hit
GTGTCAAAGGGACAGTTTATTTGGTTTGGATATGGGGACATAGGACTCCC28760.48955608021502334No Hit
GTCAAAGGGACAGTTTATTTGGTTTGGATATGGGGACATAGGACTCCCCA27870.4744063962306225No Hit
CTTCCAGGCAGGTCTGCCAGCGTTAAAGAAATTAACATGGAGAAATTGTC27140.46198025094004636No Hit
ACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTGTTATTGTTAGTAGGT25730.4379790662007146No Hit
CTCTTTTATTATGGATGCATTTGGCCACTGCTGTAATTTTTTAAGAAACA24960.42487203623668235No Hit
CACTGATACTATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAG24570.41823341067048414No Hit
GTGTCAGAAACTGCAGTCAAGCACAACCTGTCCCCAAATCCCAGCCCCTT23240.3955939952780648No Hit
GGATATGGGGACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTGTTATT22440.3819763018089404No Hit
ACTGTAAACTGTGCTTTTAGCTAAGTGAAACCTACTAACAATAACAGCCA22320.3799336477885717No Hit
GTATCAGTGTCATCATCCTTTTTAAGTTAATAATGAGCCATCGTGTTTCA21460.3652946273092629No Hit
GTTAGTAGGTTTCACTTAGCTAAAAGCACAGTTTACAGTTGCTGAAGTTT20650.35150671267177447No Hit
ATGTGGGACAGAGGCAGGTAGACTCTTCCCTAACAGGCAGCAGCAGGATT19950.33959123088629056No Hit
GCCTGGAAGGGACCTATAAATAGTATCAGTGTCATCATCCTTTTTAAGTT18770.31950513301933203No Hit
GTTATTGTTAGTAGGTTTCACTTAGCTAAAAGCACAGTTTACAGTTGCTG18540.31559004614695874No Hit
TTATTAACTTAAAAAGGATGATGACACTGATACTATTTATAGGTCCCTTC17780.3026532373512905No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA17500.297887044637097No Hit
CACTAATGTTTTCCAGTTGACAATTTCTCCATGTTAATTTCTTTAACGCT17480.2975466023003689No Hit
AGCACAACCTGTCCCCAAATCCCAGCCCCTTGAAACACGATGGCTCATTA16920.28801421687198175No Hit
GTTCTCATCTCAACCAAGTTCCAAATGCTAACGTATCAGTGTCAGAAACT16820.2863120051883412No Hit
GGATTATGGCCACAGTGTCAAAGGGACAGTTTATTTGGTTTGGATATGGG16420.279503158453779No Hit
ATTATGGCCACAGTGTCAAAGGGACAGTTTATTTGGTTTGGATATGGGGA16350.2783116102752306No Hit
CCAGAAAGGTGGGCAAGTGGCTGTTATTGTTAGTAGGTTTCACTTAGCTA16210.27592851391813383No Hit
CTGTTATTGTTAGTAGGTTTCACTTAGCTAAAAGCACAGTTTACAGTTGC15700.267247234331567No Hit
CTTCCCTAACAGGCAGCAGCAGGATTATGGCCACAGTGTCAAAGGGACAG15210.2589063970817283No Hit
ACATTAGTGTTTATCAACAGATCAATCAAACTTCAGCAACTGTAAACTGT15210.2589063970817283No Hit
GTTTATCAACAGATCAATCAAACTTCAGCAACTGTAAACTGTGCTTTTAG15040.2560126372195394No Hit
GCTCTCTTTTATTATGGATGCATTTGGCCACTGCTGTAATTTTTTAAGAA14640.24920379048497715No Hit
GCTAAAAGCACAGTTTACAGTTGCTGAAGTTTGATTGATCTGTTGATAAA14050.23916074155149789No Hit
CTTAAAAAGGATGATGACACTGATACTATTTATAGGTCCCTTCCAGGCAG13750.23405410650057618No Hit
GCGTTAAAGAAATTAACATGGAGAAATTGTCAACTGGAAAACATTAGTGT13250.2255430480823734No Hit
CTGCTGTAATTTTTTAAGAAACATGTTCTCATCTCAACCAAGTTCCAAAT12940.22026619186308768No Hit
GGACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTGTTATTGTTAGTAG12800.21788309550599094No Hit
AATTATGAACAGCTCTCTTTTATTATGGATGCATTTGGCCACTGCTGTAA12590.2143084509703458No Hit
TCCCAGCCCCTTGAAACACGATGGCTCATTATTAACTTAAAAAGGATGAT12570.21396800863361767No Hit
GGTAGACTCTTCCCTAACAGGCAGCAGCAGGATTATGGCCACAGTGTCAA12190.20749960423578356No Hit
GGACAGAGGCAGGTAGACTCTTCCCTAACAGGCAGCAGCAGGATTATGGC11990.20409518086850242No Hit
CCATTTGACTCCTGGATGTGGGACAGAGGCAGGTAGACTCTTCCCTAACA11950.2034142961950462No Hit
CTTTAACGCTGGCAGACCTGCCTGGAAGGGACCTATAAATAGTATCAGTG11870.2020525268481338No Hit
ACACTAATGTTTTCCAGTTGACAATTTCTCCATGTTAATTTCTTTAACGC11860.2018823056797697No Hit
GCTGTAATTTTTTAAGAAACATGTTCTCATCTCAACCAAGTTCCAAATGC11750.20000987282776514No Hit
TTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAGAAATTAACATGG11640.19813743997576053No Hit
CTCCTGGATGTGGGACAGAGGCAGGTAGACTCTTCCCTAACAGGCAGCAG11360.19337124726156696No Hit
GAGGCAGGTAGACTCTTCCCTAACAGGCAGCAGCAGGATTATGGCCACAG10750.18298775599135958No Hit
CATCTCAACCAAGTTCCAAATGCTAACGTATCAGTGTCAGAAACTGCAGT10680.1817962078128112No Hit
TTCTGACACTGATACGTTAGCATTTGGAACTTGGTTGAGATGAGAACATG10630.18094510197099092No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT10300.17532780341497708No Hit
GATAAACACTAATGTTTTCCAGTTGACAATTTCTCCATGTTAATTTCTTT10270.17481713990988493No Hit
CAGGTAGACTCTTCCCTAACAGGCAGCAGCAGGATTATGGCCACAGTGTC9990.17005094719569136No Hit
CATTAGTGTTTATCAACAGATCAATCAAACTTCAGCAACTGTAAACTGTG9920.16885939901714297No Hit
CTATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAGAAATTAAC9900.16851895668041486No Hit
ATGCTAACGTATCAGTGTCAGAAACTGCAGTCAAGCACAACCTGTCCCCA9660.16443364863967755No Hit
CTGATACTATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAGAA9620.16375276396622132No Hit
GATATGGGGACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTGTTATTG9560.16273143695603698No Hit
GTCAAGCACAACCTGTCCCCAAATCCCAGCCCCTTGAAACACGATGGCTC9560.16273143695603698No Hit
GGTTTGGATATGGGGACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTG9410.16017811943057614No Hit
ATAATGAGCCATCGTGTTTCAAGGGGCTGGGATTTGGGGACAGGTTGTGC9390.15983767709384805No Hit
CCCTAACAGGCAGCAGCAGGATTATGGCCACAGTGTCAAAGGGACAGTTT9350.15915679242039182No Hit
GCTCATTATTAACTTAAAAAGGATGATGACACTGATACTATTTATAGGTC9220.1569439172316591No Hit
AACATGTTCTCATCTCAACCAAGTTCCAAATGCTAACGTATCAGTGTCAG9150.1557523690531107No Hit
TGATGACACTGATACTATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCG9070.15439059970619826No Hit
CCCCAAATCCCAGCCCCTTGAAACACGATGGCTCATTATTAACTTAAAAA8800.14979462816036876No Hit
GTCAGAAACTGCAGTCAAGCACAACCTGTCCCCAAATCCCAGCCCCTTGA8710.14826263764509226No Hit
CTGTAATGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTC8460.14400710843599088No Hit
GTCATCATCCTTTTTAAGTTAATAATGAGCCATCGTGTTTCAAGGGGCTG8420.14332622376253465No Hit
CCTGGATGTGGGACAGAGGCAGGTAGACTCTTCCCTAACAGGCAGCAGCA8420.14332622376253465No Hit
CAGTTTACAGTTGCTGAAGTTTGATTGATCTGTTGATAAACACTAATGTT8380.14264533908907845No Hit
GTATCAGTGTCAGAAACTGCAGTCAAGCACAACCTGTCCCCAAATCCCAG8340.14196445441562222No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA8270.14077290623707384No Hit
GGCAGGTAGACTCTTCCCTAACAGGCAGCAGCAGGATTATGGCCACAGTG8250.14043246390034572No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA8160.13890047338506922No Hit
GTGTCATCATCCTTTTTAAGTTAATAATGAGCCATCGTGTTTCAAGGGGC7990.13600671352288027No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC7910.13464494417596784No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA7850.1336236171657835No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA7830.13328317482905538No Hit
CATTATTAGTGATATGTACGACTCTAAGACTAAAAATGTTACAAAAGAAA7780.1324320689872351No Hit
TAGTATCAGTGTCATCATCCTTTTTAAGTTAATAATGAGCCATCGTGTTT7770.13226184781887107No Hit
CCTTGAAACACGATGGCTCATTATTAACTTAAAAAGGATGATGACACTGA7670.1305596361352305No Hit
GGTTGTGCTTGACTGCAGTTTCTGACACTGATACGTTAGCATTTGGAACT7670.1305596361352305No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC7670.1305596361352305No Hit
GTTCCAAATGCTAACGTATCAGTGTCAGAAACTGCAGTCAAGCACAACCT7600.12936808795668212No Hit
AGTTAATAATGAGCCATCGTGTTTCAAGGGGCTGGGATTTGGGGACAGGT7550.12851698211486184No Hit
CAGCAGGATTATGGCCACAGTGTCAAAGGGACAGTTTATTTGGTTTGGAT7500.12766587627304157No Hit
ATCAATTTCCATTTGACTCCTGGATGTGGGACAGAGGCAGGTAGACTCTT7240.12324012589557613No Hit
GGGCTGGGATTTGGGGACAGGTTGTGCTTGACTGCAGTTTCTGACACTGA7230.12306990472721208No Hit
TGACACCACCATCAATTTCCATTTGACTCCTGGATGTGGGACAGAGGCAG7180.12221879888539178No Hit
CTGTAAACTGTGCTTTTAGCTAAGTGAAACCTACTAACAATAACAGCCAC7120.12119747187520746No Hit
GTTTCACTTAGCTAAAAGCACAGTTTACAGTTGCTGAAGTTTGATTGATC7050.12000592369665908No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT7030.11966548135993096No Hit
GATACTATTTATAGGTCCCTTCCAGGCAGGTCTGCCAGCGTTAAAGAAAT7000.11915481785483878No Hit
TCTCAACCAAGTTCCAAATGCTAACGTATCAGTGTCAGAAACTGCAGTCA6840.1164312791610139No Hit
GTTAATAATGAGCCATCGTGTTTCAAGGGGCTGGGATTTGGGGACAGGTT6790.11558017331919364No Hit
TTTCTGGGGAGTCCTATGTCCCCATATCCAAACCAAATAAACTGTCCCTT6750.1148992886457374No Hit
GGCCACTGCTGTAATTTTTTAAGAAACATGTTCTCATCTCAACCAAGTTC6730.11455884630900928No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC6720.11438862514064525No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA6600.11234597112027658No Hit
GGATGATGACACTGATACTATTTATAGGTCCCTTCCAGGCAGGTCTGCCA6550.11149486527845628No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG6510.11081398060500008No Hit
GTTTCAAGGGGCTGGGATTTGGGGACAGGTTGTGCTTGACTGCAGTTTCT6500.11064375943663603No Hit
GTGTTTATCAACAGATCAATCAAACTTCAGCAACTGTAAACTGTGCTTTT6420.10928199008972359No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC6380.10860110541626734No Hit
ATTATGGATGCATTTGGCCACTGCTGTAATTTTTTAAGAAACATGTTCTC6360.10826066307953926No Hit
GAACAGCTCTCTTTTATTATGGATGCATTTGGCCACTGCTGTAATTTTTT6340.10792022074281114No Hit
GAAATTAACATGGAGAAATTGTCAACTGGAAAACATTAGTGTTTATCAAC6300.1072393360693549No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC6290.10706911490099084No Hit
GTTAATTTCTTTAACGCTGGCAGACCTGCCTGGAAGGGACCTATAAATAG6290.10706911490099084No Hit
CCATCAATTTCCATTTGACTCCTGGATGTGGGACAGAGGCAGGTAGACTC6240.10621800905917059No Hit
GGATGTGGGACAGAGGCAGGTAGACTCTTCCCTAACAGGCAGCAGCAGGA6240.10621800905917059No Hit
GTTTGGATATGGGGACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTGT6200.10553712438571435No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT6170.1050264608806222No Hit
CCACCATCAATTTCCATTTGACTCCTGGATGTGGGACAGAGGCAGGTAGA6120.1041753550388019No Hit
GGCTGTTATTGTTAGTAGGTTTCACTTAGCTAAAAGCACAGTTTACAGTT6090.10366469153370976No Hit
AAACACGATGGCTCATTATTAACTTAAAAAGGATGATGACACTGATACTA6060.10315402802861759No Hit
ATGCTTAACCCTAATTATGAACAGCTCTCTTTTATTATGGATGCATTTGG6000.10213270101843326No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA5990.1019624798500692No Hit
CATGTTCTCATCTCAACCAAGTTCCAAATGCTAACGTATCAGTGTCAGAA5940.1011113740082489No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC5940.1011113740082489No Hit
GACATAGGACTCCCCAGAAAGGTGGGCAAGTGGCTGTTATTGTTAGTAGG5920.10077093167152082No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA5910.10060071050315676No Hit
GACCTATAAATAGTATCAGTGTCATCATCCTTTTTAAGTTAATAATGAGC5900.1004304893347927No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAT151.229749E-4145.08
TCAACGG151.229749E-4145.06
AGCCGTC100.007090124144.999987
ACCGCAT100.007090124144.99998145
TTGTCGG100.007090124144.999986
TCGTCAG100.007090124144.999986
TCCCCGA100.007090124144.999986
CGTCAGT100.007090124144.999987
TGTCGGT100.007090124144.999987
GCCGTCG100.007090124144.999988
GCTTAAT301.1011434E-7120.8333361
TGCTTAA6250.0103.2400051
GACCGAG301.5989075E-596.6666647
GGACCGA301.5989075E-596.6666646
CCTATAT301.5989075E-596.6666641
GTTGACT259.3848055E-487.01
GTGATAC259.3848055E-487.03
TGACGAC259.3848055E-487.04
GACGACA259.3848055E-487.05
ACGACAC259.3848055E-487.06
Produced by FastQC (version 0.11.5)