FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_35_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_35_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594709
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTT88781.4928309475726784No Hit
GTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC69191.1634261462328634No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCC63581.069094296538307No Hit
GGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAA62511.0511023038158158No Hit
GGTTCAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA53220.8948914511130655No Hit
CCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTCACCAATA51030.858066718344602No Hit
CCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG45550.7659208116910959No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATAT39760.6685622716320083No Hit
CACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA35260.5928950125187277No Hit
CCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACC33790.5681770412083893No Hit
CATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAG32160.5407686784629121No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAA31600.5313523084399261No Hit
GTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCAT27000.4540035546796837No Hit
CAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCAC26100.43887010285702754No Hit
GCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTT26060.43819750499824284No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG24930.4191966154875746No Hit
CCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC22620.3803540891427572No Hit
CAATACCAATGTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTG22400.37665480091944126No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT21420.3601761533792157No Hit
GTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCA20420.3433612069095978No Hit
CCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGAC20310.3415115627979398No Hit
CCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAACT19420.3265462604399799No Hit
CCAATGTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTGGGTGT18410.3095631645056658No Hit
CTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGC18300.30771352039400784No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATATTGGTG17970.3021645880590339No Hit
GGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATA17230.28972152767151665No Hit
CAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGTTG16350.2749243747782529No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT16130.27122508655493693No Hit
GTTCACCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTCAC15930.26786209726101334No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT15170.25508273794410374No Hit
ATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCAC14630.2460026668505101No Hit
ATACCAATGTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTGGG14040.2360818484334355No Hit
ATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCA13920.23406405485708134No Hit
GCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCC13870.2332233075336005No Hit
CACAATACCAATGTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTT13830.23255070967481573No Hit
GTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAACTG13740.23103736449255016No Hit
GTTCAGTTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAG13650.22952401931028454No Hit
GTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA13630.2291877203808922No Hit
GGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACA12810.2153994642758055No Hit
CAATACCAATGTTCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTG12560.21119572765840097No Hit
CAATTACCAATGTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTTT11880.19976156405906081No Hit
AACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCG11850.19925711566497228No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC11710.19690302315922575No Hit
TCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCG11440.19236298761242893No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG11410.1918585392183404No Hit
GAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTG11120.1869822047421512No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC10910.18345106598353145No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA10730.1804243756190002No Hit
ATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGT10460.17588434007220338No Hit
ATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTC10400.17487544328402632No Hit
GGTGAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATATTGGTGAA10330.17369839703115306No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGGTGGGCCTCATAGCACA10220.17184875291949508No Hit
GGTTCACAGATGCGCAAACAGGTTCACTGTTCCTGGAATATTGGTGAACA10090.16966280987844476No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG10080.16949466041374858No Hit
CTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGC9920.16680426897860973No Hit
CACCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCG9900.16646797004921735No Hit
TTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAA9900.16646797004921735No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAAC9420.15839679574380075No Hit
AACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCAA9410.15822864627910457No Hit
CATCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTG9370.15755604842031984No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGT9210.15486565698518098No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA8910.1498211730442956No Hit
GGTATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACAAA8880.1493167246502071No Hit
ATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTG8840.14864412679142236No Hit
GGGTAATAATGACTTGTTGGTGATTGTAGATATTGGGCTGTTAATTGTCA8820.14830782786203No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT8800.14797152893263762No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT8770.14746708053854912No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT8650.14544928696219495No Hit
GGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGTTGG8570.14410409124462553No Hit
ATACCAATGTTCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTGGG8560.14393594177992936No Hit
ACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCAC8530.1434314933858408No Hit
ATGTTCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTGGGTGTCTG8520.14326334392114462No Hit
ACTATGGGTGTTAAATTTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG8410.14141369980948665No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT8330.1400685040919172No Hit
TGATAGAGCCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTG8070.13569661800981656No Hit
CACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTG8030.13502402015103185No Hit
GTGCTATGAGGCCCACCAATTTTGTAATGATCCATTTTTGGGTGTCTGGA7890.13266992764528535No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG7870.132333628715893No Hit
TGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACA7870.132333628715893No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTTACTCTCTCTACAAGGTT7860.1321654792511968No Hit
CAATACAGGTTCATCGGTGCCTGGAATATTGGTGAACAGATGCGCAAACA7790.13098843299832355No Hit
CTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGT7580.12745729423970378No Hit
CAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTG7470.1256076501280458No Hit
TCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAAT7450.12527135119865346No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAG7430.12493505226926109No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC7240.1217402124400337No Hit
GATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAA7210.12123576404594516No Hit
GGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTC7190.1208994651165528No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA7140.12005871779307191No Hit
GTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATAT7100.11938611993428719No Hit
AGCCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTG7030.11820907368141395No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGT7000.11770462528732539No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC7000.11770462528732539No Hit
TACTATGGGTGTTAAATTTTTTTACTCTCTCTACAAGGTTTTTTCCTAGT6900.1160231306403636No Hit
TCTGTTCACCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTT6880.11568683171097126No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC6770.11383718759931329No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC6760.1136690381346171No Hit
GAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCAATGTG6720.11299644027583239No Hit
CCCAAAAATGGATCATTACAAAATTGGTGGGCCTCATAGAACATTGGTAT6700.11266014134644002No Hit
ATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA6660.11198754348765531No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA6610.11114679616417442No Hit
TGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC6540.10996974991130115No Hit
TTACAAAATTGGTGGGCCTCATAGAACATTGGTATTGTGGGCTCAATGTG6430.10812010579964318No Hit
GGCACCAACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC6400.10761565740555466No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG6380.10727935847616228No Hit
ATTTTGTAATGATCCATTTTTGGGTGTCTGGACACATTGAGCCCACAATA6370.1071112090114661No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCAACTGAACCTGTTTGC6370.1071112090114661No Hit
CTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC6320.10627046168798521No Hit
CTGTTCACCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTGTTC6280.10559786382920049No Hit
GCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAG6230.10475711650571959No Hit
CACAATACCAATGTTCTATGAGGCCCACCAATTTTGTAATGATCCATTTT6220.10458896704102343No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA6210.10442081757632725No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC6140.103243771323454No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT6100.10257117346466928No Hit
CATCTGTTCACCAATATTCCAGGCACCAACTGAACCTGTTTGCGCATCTG6050.10173042614118838No Hit
CCCAAAAATGGATCATTACAAAATTGGTGGGCCTCATAGCACATTGGTAT5990.1007215293530113No Hit
GAACAGATGCGCAAACAGGTTCAGTTGGTGCCTGGAATATTGGTGAACAG5980.10055337988831513No Hit
CATTGAGCCCACAATACCAATGTTCTATGAGGCCCACCAATTTTGTAATG5960.10021708095892276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGT253.709647E-8145.01
ACACCCG151.2297566E-4145.05
CACCCGA151.2297566E-4145.06
GTAAAGG151.2297566E-4145.08
CAGCGCG100.0070901453144.99998145
GGGTATG100.0070901453144.999987
GAGACCC202.129891E-6144.999988
TACGAGG202.129891E-6144.999985
GAGCCCG100.0070901453144.999985
ACGTCCC100.0070901453144.999983
GCGGCCC100.0070901453144.999989
TGCTACG202.129891E-6144.999982
GATTAGT100.0070901453144.999987
CCTAGTA351.900844E-9124.285712
TATTCCG1050.0124.285715
CGAGGCC256.462702E-6116.07
AGACCCA256.462702E-6116.09
GAAGGGT256.462702E-6116.01
ATTCCGG1150.0113.4782646
TCCGGGC1150.0113.4782648
Produced by FastQC (version 0.11.5)