FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_35_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_35_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695502
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGT100491.4448556582152172No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAA82141.1810174521424812No Hit
TCCCTATGGTGCTACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATG66930.9623264922315103No Hit
TCCCTATGGTGCTAACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGG59720.8586603633059288No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGTAGCACCATAGGG54510.7837504421266942No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACC54030.7768489522675708No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTTAGCACCATAGGGA44010.6327803514583711No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAAAGCACTCGTGGACA41400.5952535003493878No Hit
TCCCTATGGTGCTACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATG38460.5529818749622575No Hit
GTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGT38050.5470868523742562No Hit
GTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCAGCTGT37800.5434923264059629No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGTAGCA36340.5225002947511294No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGGGTAGCACCATAGGG34510.4961883646632217No Hit
CAGCTGGACTTCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTGGACT32760.47102668288516786No Hit
TCCCTATGGTGCTACAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTAT29170.41940928998047455No Hit
GTCCAGCTGTCCACGAGTGCTTTGCAGTAGCACCATAGGGAAGTCCAGCT29020.41725257439949853No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTTAGCAC28840.4146645157023272No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTGTAGCACCATAGG28570.41078242765657036No Hit
GTCCACGAGTGCTTTGCAGTAGCACCATAGGGAAGTCCAGCTGTCCACGA27390.39381626508622547No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTACTGCAAAGCACTCGTGGA26530.3814510957552962No Hit
GTGCTACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACC24830.35700831917090103No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTAACCAAAGCACTCGTGGAC24180.34766255165333815No Hit
GTGCTACAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAC23930.34406802568504474No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGGGTAGCA23370.3360162875160675No Hit
GTCCACGAGTGCTTTGGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAG22900.32925857869567593No Hit
GTGCTACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACC21820.3137302265126484No Hit
CAGCTGGACTTCCCTATGGTGCTACTGCAAAGCACTCGTGGACAGCTGGA20430.2937446621289371No Hit
CAGCTGGACTTCCCTATGGTGCTAACCAAAGCACTCGTGGACAGCTGGAC19590.2816670548754713No Hit
GTCCAGCTGTCCACGAGTGCTTTGGGGTAGCACCATAGGGAAGTCCAGCT19020.2734715356677623No Hit
GTCCACGAGTGCTTTGGGGTAGCACCATAGGGAAGTCCAGCTGTCCACGA18970.2727526304741036No Hit
GTCCAGCTGTCCACGAGTGCTTTGGTTAGCACCATAGGGAAGTCCAGCTG18920.2720337252804449No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTGTAGC18690.268726761389615No Hit
GCTGGACTTCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTGGACTTC18540.26657004580863897No Hit
ACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAAAGC18350.26383820607273595No Hit
TCCCTATGGTGCTACCAGCAAAGCACTCGTGGACAGCTGGACTTCCCTAT18270.26268795776288206No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTACCCCAAAGCACTCGTGGA17250.24802229181224497No Hit
ACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAAAGC16920.24327751753409768No Hit
TCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTA16820.24183970714678032No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCTGGTAGCACCATAGG16380.23551334144258393No Hit
GTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAA16010.2301934430095097No Hit
GGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACCA15820.2274616032736067No Hit
GTCCAGCTGTCCACGAGTGCTTTGGCTGTAGCACCATAGGGAAGTCCAGC15730.22616757392502107No Hit
GCTAGCTGGACTTCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTGGA14230.20460041811526064No Hit
GTCCACGAGTGCTTTGGCTGTAGCACCATAGGGAAGTCCAGCTGTCCACG14150.20345016980540676No Hit
CCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGTAGCACCATAG14000.20129345422443068No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGT13980.2010058921469672No Hit
TTGCAGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGC13950.20057454903077204No Hit
ATGGTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAC13370.19223524878433132No Hit
CAGCTGGACTTCCCTATGGTGCTACCCCAAAGCACTCGTGGACAGCTGGA13240.19036609528081874No Hit
GTCCAGCTAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCAG12980.18662778827379362No Hit
TTGGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCA12910.18562132100267145No Hit
GTGCTAACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCA12640.18173923295691458No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTACAGCCAAAGCACTCGTGG12600.18116410880198763No Hit
ACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACTGCAAA12320.177138239717499No Hit
GCTTTGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAG12090.17383127582666907No Hit
GCTGGACTTCCCTATGGTGCTACTGCAAAGCACTCGTGGACAGCTGGACT11970.17210590336188825No Hit
GCTGGACTTCCCTATGGTGCTAACCAAAGCACTCGTGGACAGCTGGACTT11730.1686551584323266No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTGACCATAGGGAAGT11570.1663546618126188No Hit
GCTTTGCTGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGG11550.16606709973515532No Hit
TCCCTATGGTGCTACTGCAAAGCACTCGTGGACAGCTAGCTGGACTTCCC11540.1659233186964236No Hit
GTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACTGC11170.16060342026334934No Hit
GCTTTGGCTGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGG11020.1584467046823733No Hit
ACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAACCAAAG10780.15499595975281164No Hit
GGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGTAGCAC10700.15384571144295775No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCTGGTAGC10520.1512576527457865No Hit
GTGCTACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACT10390.14938849924227393No Hit
GCTTTGCAGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGT10380.14924471820354218No Hit
TCCCTATGGTGCTAACCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCT10320.14838203197115177No Hit
CTTGTGATGGTGGTGTCACTCGTGGACAGCTGGACTTCCCTATGGTGCTA10230.14708800262256616No Hit
ACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAAAGCAC10220.1469442215838344No Hit
GGTGACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATA10200.14665665950637094No Hit
GGGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCAC10140.14579397327398053No Hit
CCATAGGGAAGTCCAGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGT9990.14363725769300448No Hit
ACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACTGCAAA9810.1410491989958332No Hit
CAGCTGGACTTCCCTATGGTGCTACAGCCAAAGCACTCGTGGACAGCTGG9740.14004273172471107No Hit
ATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAAGT9670.1390362644535889No Hit
ACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAACCAAAG9520.13687954887261286No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCTAGCTGT9300.13371636602051468No Hit
CCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGCAGTAGCACCAT9270.13328502290431948No Hit
GCTAGCTGGACTTCCCTATGGTGCTACTGCAAAGCACTCGTGGACAGCTG9170.1318472125170021No Hit
GTGCTACCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATGGTGCTA9160.13170343147827038No Hit
GTGCTACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAAC9020.12969049693602608No Hit
GTCCAGCTAGCTGTCCACGAGTGCTTTGCAGTAGCACCATAGGGAAGTCC8870.12753378135505003No Hit
GTGCTACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAAC8850.12724621927758653No Hit
GCTGGACTTCCCTATGGTGCTACCCCAAAGCACTCGTGGACAGCTGGACT8830.12695865720012306No Hit
GTCCAGCTGTCCACGAGTGCTTTGCTGGTAGCACCATAGGGAAGTCCAGC8680.12480194161914704No Hit
GCTTTGGGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGT8650.12437059850295183No Hit
TTGGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGTAG8540.12278900707690273No Hit
TCCCTATGGTGCTACCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGG8490.12207010188324405No Hit
GTGCTACCCCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATGGTGC8420.12106363461212188No Hit
GTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAACCA8410.12091985357339016No Hit
GTGCTACTGCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACT8390.12063229149592669No Hit
GTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCAGCTAG8390.12063229149592669No Hit
GGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACC8140.11703776552763327No Hit
GGTTAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGTAG8130.11689398448890154No Hit
TTGCAGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGTA8120.11675020345016981No Hit
GTGCTACTGCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATGGTGC8100.11646264137270632No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAGCAAAGCACTCGTGG7940.11416214475299855No Hit
GTCCACGAGTGCTTTGCTGGTAGCACCATAGGGAAGTCCAGCTGTCCACG7890.11344323955933987No Hit
GGGAAGTCCAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCA7770.11171786709455904No Hit
GGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTTAGCACC7720.11099896190090036No Hit
GCTAGCTGGACTTCCCTATGGTGCTAACCAAAGCACTCGTGGACAGCTGG7680.1104238377459734No Hit
GCTTTGGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCAGT7660.11013627566850993No Hit
CCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGTTAGCACCATA7590.10912980839738778No Hit
GGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTGACCAT7550.10855468424246083No Hit
CTACAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAA7540.10841090320372909No Hit
GTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCAGCTAGCTGTCCAC7520.10812334112626563No Hit
GTGCTAACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACTG7500.10783577904880216No Hit
GCTACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCAA7480.10754821697133868No Hit
GCTTTGGTAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTG7460.1072606548938752No Hit
ACCCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTACCCCAAA7450.10711687385514349No Hit
GTCCAGCTAGCTGTCCACGAGTGCTTTGGTTAGCACCATAGGGAAGTCCA7270.10452881515797222No Hit
TTGCAGTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTTAG7210.1036661289255818No Hit
TCCCTATGGTGCTACCCCAAAGCACTCGTGGACAGCTAGCTGGACTTCCC7100.10208453749953271No Hit
TTGGTTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGTTAGC7080.10179697542206925No Hit
CCCTATGGTGCTACCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTG7060.10150941334460577No Hit
TCCCTATGGTGCTACCAAAGCACTCGTGGACAGCTAGCAAAGCACTCGTG7050.10136563230587403No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGGTAGCACCATAGGGA7040.1012218512671423No Hit
GTCTAGCTGTCCACGAGTGCTTTGGTAGCACCATAGGGAAGTCCAGCTGT7010.10079050815094709No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAT100.0070904023144.999981
CTGATTA100.0070904023144.999989
TGCATAG600.0132.916673
ACGAGTA1200.0126.8755
CGAGTAC1100.0125.227286
GAGTACT1100.0125.227287
ATGGTAC2300.0122.9347846
TATTGCA600.0120.8333365
GGTACTA2400.0117.81258
AGCCTAA256.4634787E-6116.03
GTACTTT1200.0114.7916649
GTACTAC1950.0111.538469
TGGTACT2550.0110.882357
TTAGTAC1850.0109.729743
ACTGCGA203.8655574E-4108.749996
CTGCGAA203.8655574E-4108.749997
TGCGAAG203.8655574E-4108.749998
AACTATG203.8655574E-4108.749995
TAACTAT203.8655574E-4108.749994
GGTTAGT1900.0106.842111
Produced by FastQC (version 0.11.5)