FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_33_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_33_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473275
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTAC28350.5990174845491522No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT23230.4908351381332206No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA22380.47287517827901326No Hit
GTCTTATTACAGTAGGCTAAGATAAGTGCACAAAAGTTAGCAGCTTCACC21370.45153452009930806No Hit
GTAATAAGCATCTTGTAGAGCAGGTGGATTAAACTTCAACTCTATTTGTT19960.4217421161058581No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC17660.37314457767682635No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT17600.37187681580476467No Hit
GTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCA16970.3585653161481169No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG16080.339760181712535No Hit
GGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTTAAGT15740.33257619777085207No Hit
TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT15630.33025196767207227No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG15510.32771644392794885No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC15360.3245470392477946No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA14110.29813533357984257No Hit
GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG14030.29644498441709366No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG13340.28186572288838413No Hit
CCCTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAA13260.2801753737256352No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT13150.2778511436268554No Hit
GCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTAT12670.2677090486503618No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT12210.2579895409645555No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC11950.25249590618562145No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG11490.2427763984998151No Hit
CATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTA11020.23284559716866515No Hit
CTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTT10910.23052136706988535No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT10560.2231260894828588No Hit
GTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTA10520.22228091490148436No Hit
AGTTCATACTGAGCAGGTGGTGCTGACATCATAACAAAAGGTGACTCCTG10360.2189002165759865No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG10280.21720986741323753No Hit
CTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTT9930.20981458982621098No Hit
CCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAA9850.20812424066346205No Hit
GGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTT9510.2009402567217791No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT9230.19502403465215784No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT9210.19460144736147061No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT9040.19100945539062913No Hit
CTCCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAG8760.18509323332100786No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC8700.18382547144894618No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC8570.18107865405947915No Hit
AGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTG8460.17875442396069938No Hit
CTATTAGCATAAGCAGTTGTGGCATCTCCTGATGAGGTTCCACCTGGTTT8270.17473984469917067No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC8200.17326078918176535No Hit
GTTTTACACACCACGTTCAAGACTCTTTTGCAAGAATCTAAATTGGCATG8070.17051397179229835No Hit
GTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATAAAGAAC8030.1696687972109239No Hit
GAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGC7980.1686123289842058No Hit
GTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGT7820.16523163065870794No Hit
GTAGATAAAAGTGCATTAACATTGGCCGTGACAGCTTGACAAATGTTAAA7790.1645977497226771No Hit
GAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTG7790.1645977497226771No Hit
GTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTA7700.16269610691458455No Hit
ATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTA7570.15994928952511753No Hit
ATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAA7550.1595267022344303No Hit
CTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAAGA7540.15931540858908666No Hit
GATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGA7470.15783635307168137No Hit
CCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATC7470.15783635307168137No Hit
GCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGAGTATCATCAT7430.1569911784903069No Hit
ACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGA7410.15656859119961966No Hit
GGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGC7390.15614600390893243No Hit
TTCTTAAATTGTTCATAAGAAAGTGTGCCCATGTACATAACAGCTTCTAC7380.15593471026358882No Hit
CGTACAAGGTATCTGAACACCTTTCTTAAATTGTTCATAAGAAAGTGTGC7290.15403306745549628No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT7260.15339918651946544No Hit
ACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAA7180.1517088373567165No Hit
CATAAAAACATTGTTTTGATAATAAAGAACTGACTTAAAGTTCTTTATGC7160.15128625006602925No Hit
CATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGA7050.14896201996724948No Hit
GCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAAC7020.14832813903121864No Hit
CACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGT6900.14579261528709525No Hit
ATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGA6850.14473614706037716No Hit
GTAAATGTACCATGCTTAAGTTCATACTGAGCAGGTGGTGCTGACATCAT6770.14304579789762822No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA6510.1375521631186942No Hit
CATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAG6510.1375521631186942No Hit
GAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCA6470.13670698853731975No Hit
GTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAG6440.13607310760128888No Hit
ATTTATAAGTAACTGGTTTTATGGTTGTTGTGTAACTGTTTTCTTTGTAG6430.13586181395594527No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC6310.13332629021182188No Hit
TTTGTATTCTGAGGACTTTGTAAGTAAAGCACCGTCTATGCAATACAAAG6310.13332629021182188No Hit
CATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGT6290.13290370292113463No Hit
GTTTATAATCAATAGCCACCACATCACCATTTAAGTCAGGGAAAAATGTA6280.132692409275791No Hit
TTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACAC6280.132692409275791No Hit
GTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCACA6270.1324811156304474No Hit
GTTTTATGGTTGTTGTGTAACTGTTTTCTTTGTAGAAAACATCCGTAATA6190.13079076646769847No Hit
GTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTG6150.129945591886324No Hit
ATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGAC6130.1295230045956368No Hit
ATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTAT6110.12910041730494956No Hit
GTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGT6070.12825524272357508No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC6040.12762136178754424No Hit
CCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAG6040.12762136178754424No Hit
AATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGA5910.12487454439807721No Hit
CTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAA5910.12487454439807721No Hit
GTTCAAGACTCTTTTGCAAGAATCTAAATTGGCATGTTGAAACAAGTAAC5880.12424066346204637No Hit
GTCAGAGAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACT5870.12402936981670276No Hit
CCCTAGCATTGTTAACATGCCAAACAATAGGTTTATGTAACAATTTAGCT5850.12360678252601554No Hit
TCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACG5780.12212772700861022No Hit
CACTAGCACAAGTAAATGTACCATGCTTAAGTTCATACTGAGCAGGTGGT5760.12170513971792299No Hit
CTTATAACCAGTTAACTGGTTTAAATCATCAGCAAATTTGATATTATCAC5740.12128255242723576No Hit
GTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTT5720.12085996513654851No Hit
GCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTC5690.12022608420051767No Hit
ACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGA5680.12001479055517406No Hit
GAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGC5640.11916961597379959No Hit
GTCCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAA5630.11895832232845598No Hit
CTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACA5630.11895832232845598No Hit
GAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACAC5600.11832444139242512No Hit
CTCTATTTCTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGACA5540.11705667952036343No Hit
AGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGG5530.11684538587501982No Hit
GTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATC5440.11494374306692727No Hit
CTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGT5380.11367598119486556No Hit
GACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTC5370.11346468754952195No Hit
CAACAGCATCGTCAGAGAGTATCATCATTGAGAAATGTTTACGCAAATAT5320.11240821932280386No Hit
GTACTAGATATTTTGTAGCTTGTTTACCACACGTACAAGGTATCTGAACA5290.11177433838677302No Hit
CACTGGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTT5190.10966140193333686No Hit
GTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTACCATCAGTAG5150.1088162273519624No Hit
GCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTT5140.10860493370661876No Hit
GTTATATGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGC5140.10860493370661876No Hit
CTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATC5050.10670329089852623No Hit
AGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGT5040.1064919972531826No Hit
GCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATA5040.1064919972531826No Hit
CCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTC5020.10606940996249538No Hit
GTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACA4990.10543552902646453No Hit
TAATAGGACCTTTGTATTCTGAGGACTTTGTAAGTAAAGCACCGTCTATG4990.10543552902646453No Hit
AGATAAAAGTGCATTAACATTGGCCGTGACAGCTTGACAAATGTTAAAAA4990.10543552902646453No Hit
GTGCTATTGAAACACACAACAGCATCGTCAGAGAGTATCATCATTGAGAA4940.10437906079974645No Hit
TATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAG4910.10374517986371559No Hit
GTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATA4880.10311129892768475No Hit
GTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCAC4880.10311129892768475No Hit
ATCACATACAAACTTAAAATTATCGAAGCTTGCGTTTGGATATGGTTGGT4780.10099836247424859No Hit
CAATAGGTTTATGTAACAATTTAGCTCCTTTCTTAAAAGAGGGTGTGTAG4780.10099836247424859No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCC100.00708969145.08
AGCCGTG100.00708969145.07
AACCGGG100.00708969145.07
ACCGGGT151.2295993E-4145.08
GTCGAGG100.00708969145.05
GTCGAGA100.00708969145.05
GATCGAT100.00708969145.07
CCCTAGT202.1294345E-6145.01
CCGTGCC100.00708969145.09
TACGGTT100.00708969145.05
GTAGGGT100.00708969145.06
GGGAGTC100.00708969145.07
TGCATTC100.00708969145.05
AAACGTC100.00708969145.07
GCATTCT100.00708969145.06
ACAGGGG100.00708969145.06
GTGTCGA100.00708969145.03
ACGTGTC100.00708969145.01
AAACCGG100.00708969145.06
TAGGGTG100.00708969145.07
Produced by FastQC (version 0.11.5)