FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_33_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_33_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624357
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT24530.392884199264203No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG20230.3240133449292632No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA19980.3200092254911853No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG18510.2964650031952873No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA17980.28797626998656217No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT15060.24120815494981235No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC14420.23095760918833297No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG12820.20533124478463444No Hit
GGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGC12380.19828399457361734No Hit
ATAATACCCATAGCAAAAGGTAAAAAGGCATTTTCATACAAAAAAAAGAA11290.18082603382359771No Hit
GATTATAAGAGCCCACATGGAAATGGCTTGATCTAAAGCATTACCATAAT10990.17602109049790424No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA10760.1723373006148726No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC10710.171536476727257No Hit
GTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAA10450.167372192511656No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC10380.1662510390689942No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG10340.1656103799589017No Hit
GTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTT10270.1644892265162399No Hit
ACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAG10270.1644892265162399No Hit
CATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAA10240.16400873218367057No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA9980.15984444796806954No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC9970.15968428319054645No Hit
CTCTTAAGAAGTTGAATGTCTTCACCTTTGTTAACATTTGGGCCGACAAC9870.15808263541531528No Hit
CTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGA9850.15776230586026904No Hit
TTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA9710.15551999897494542No Hit
GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT9690.15519966941989918No Hit
GTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCAT9460.15151587953686754No Hit
CTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTT9410.15071505564925194No Hit
GGCATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAA9220.14767192487631275No Hit
CATAAAAACAATACCTCTGGCCAAAAACATGACAGTTGTAACTACACCTG9210.14751176009878963No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA9190.1471914305437434No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA9170.14687110098869718No Hit
CTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCTGATAATAAT9110.14591011232355847No Hit
ACATAACACAGTCTTTTAGCTTAAAACCAGACAAACTAGTATCAACCATA8950.14334747588318864No Hit
ATTCATAAGTGTCCACACTCTCCTAGCACCATCATCATACACAGTTCTTG8910.14270681677309616No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC8750.1401441803327263No Hit
GTATCTACACAAACTCTTAAAGAATGTATAGGGTCAGCACCAAAAATACC8500.13614006089464842No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT8410.13469857789694037No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA8380.134218083564371No Hit
ATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCT8290.132776600566663No Hit
GCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC8220.13165544712400118No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA8170.13085462323638558No Hit
GTCTAAAGTAGCGGTTGAGTAAACAAAAGAGGCCAAAGTAACAAGTACAA8110.1298936345712469No Hit
GCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATT8110.1298936345712469No Hit
TCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCAC8070.12925297546115444No Hit
ATCTAAAGCATTACCATAATAAACTTTATAAACGAGTGTCAAGACATTCA8070.12925297546115444No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG7910.12669033902078458No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG7900.12653017424326146No Hit
GTCTAACAACATCAAAAGGTGTAAATTCATCTTCTAATAAAGCACTACCC7730.12380737302536848No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT7690.12316671391527603No Hit
ATTGAGTACTCTGGACTAAAACTAAAAGTGAAGTCAAAATTGTGAGTAAC7590.12156506614004486No Hit
CTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG7570.12124473658499865No Hit
ATTTTAGACTGTACAGTGGCTACTTTGATACAAGGTTTGCCACCAACACC7260.11627962848178205No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC7240.11595929892673582No Hit
AACTAGTATCAACCATATCCAACCATGTCATAATACGCATCACCCAACTA7240.11595929892673582No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC7230.11579913414921271No Hit
CTTGTAGATCTGTTCTCTAAACGAACATGAAAATTATTCTTTTCTTGGCA7210.11547880459416648No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC7200.11531863981664336No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT7130.11419748637398155No Hit
GTAGTATCTTTAGCTAAGAGAATGTCATTGTGTAACTGGACACATTGAGC7120.11403732159645844No Hit
GTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCT7070.11323649770884286No Hit
GATAATAATGGTGCAAGTAGAACTTCGTGCTGATTAAAATTTTCATAAGC7040.1127560033762735No Hit
GTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGA6960.11147468515608858No Hit
GTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTT6850.10971287260333432No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG6700.10731040094048758No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT6610.10586891794277953No Hit
CAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAAT6500.10410710539002525No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT6500.10410710539002525No Hit
ATTTAATGTTGAGTTTGAAGGCATCTATGCTATTCTTGGGTGGGAGTAGT6440.10314611672488656No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT6430.10298595194736344No Hit
CCATTAAGGAATGAACCCTTAATAGTGAAATTGGGCCTCATAGCACATTG6370.10202496328222475No Hit
GGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTG6290.10074364506203982No Hit
TACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTGT6290.10074364506203982No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT6250.10010298595194737No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGT100.0070902277145.000021
TAGAGGG100.0070902277145.000027
ACACCGA100.0070902277145.000023
ATAGAGG100.0070902277145.000026
CTGACAG100.0070902277145.000025
CAGGCTT100.0070902277145.000027
TCCCGCC100.0070902277145.000027
ACTCCCG100.0070902277145.000025
TAGGAGT100.0070902277145.000026
ATGCCCA100.0070902277145.000029
TTACCGG100.0070902277145.000026
GTGCGCA100.0070902277145.000029
TACACGA100.0070902277145.000026
GGCCGTG151.2297858E-4145.01
TGTATCC151.2297858E-4145.08
TTACGCA151.2297858E-4145.07
GTATCCA203.8653685E-4108.750019
TTCTGAG203.8653685E-4108.750017
CAGGTAT501.9463187E-10101.54
GTCATAT651.6370905E-1189.230771
Produced by FastQC (version 0.11.5)