FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_32_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_32_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456763
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT54291.1885813868461323No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG48311.0576600994388774No Hit
CCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGAC47141.0320450649461537No Hit
ATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGT40650.889958249683096No Hit
CCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATT33410.7314515405144462No Hit
GTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA29500.6458491602866256No Hit
GGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACG26330.5764477420456561No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC25570.5598089162213227No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTG24470.5357264051597875No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT24100.527625924166362No Hit
CCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAAC23740.5197443750916777No Hit
CACCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCG23380.5118628260169935No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA22110.48405847233685745No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT21260.46544925924385294No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA20650.45209441220063795No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT20500.44881043341951954No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT20020.43830170131994056No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT19420.4251657861954668No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC18350.4017400708901553No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT17850.3907934749530938No Hit
ATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG17710.3877284280907166No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC16940.370870670347642No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG16150.3535750487670849No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT16140.35335611684834367No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC15980.34985320614848403No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG15130.3312439930554795No Hit
ATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA14480.3170134183372997No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC14450.31635662258107594No Hit
CTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC14400.3152619629873698No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT13740.30081245635044873No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC13580.29730954565058904No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC13480.29512022646317676No Hit
ATTTTTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAG13320.29161731576331706No Hit
TGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC13160.2881144050634574No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC12980.2841736305261153No Hit
GTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCA12080.2644697578394047No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT11860.2596532556270976No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAA10730.23491394880933875No Hit
GCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTT10040.21980764641619396No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG9980.2184940549037466No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT9920.21718046339129923No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC9810.21477221228514567No Hit
CAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCT9730.21302075693521585No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAA9650.21126930158528603No Hit
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC9590.20995571007283864No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA9430.206452799372979No Hit
GTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAA9020.19747659070458856No Hit
TCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGG8990.19681979494836488No Hit
GTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGAT8880.19441154384221138No Hit
TCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG8870.19419261192347015No Hit
ATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCG8770.19200329273605787No Hit
GGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGAT8540.1869678586050096No Hit
ATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA8540.1869678586050096No Hit
GGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCT8530.18674892668626838No Hit
TTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTA8390.18368387982389117No Hit
GATCCATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAG8390.18368387982389117No Hit
CCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTT8340.182589220230185No Hit
GTCTAGGTTTACCAACTTCCCATGAAAGATGTAATTCTCTGTCAGACAGC8160.1786484456928429No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT8000.17514553499298324No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA7800.17076689661815864No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT7320.16025816451857966No Hit
GTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGA7190.15741204957494367No Hit
GTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCT6860.15018729625648314No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGC6850.1499683643377419No Hit
GATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC6830.14953050050025946No Hit
GGGTAAGGAAGGTACACATAATCATCACCCTGTTTAACTAGCATTGTATG6660.14580865788165853No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC6480.1418678833443164No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT6460.14143001950683395No Hit
GTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATT6410.14033535991312782No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG6120.1339863342696322No Hit
TTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAA6090.13332953851340848No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG6070.13289167467592602No Hit
GAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCT6020.1317970150822199No Hit
AGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGA6000.13135915124473743No Hit
CTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTT5960.13048342356977252No Hit
ATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTC5960.13048342356977252No Hit
GGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAG5930.1298266278135488No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG5870.12851303630110145No Hit
CTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCAC5720.125229057519983No Hit
CATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTT5710.12501012560124178No Hit
GTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG5680.1243533298450181No Hit
TACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAAC5630.12325867025131194No Hit
TCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCTGTTCA5600.12260187449508826No Hit
ACATAAGAATGGTCTACGTATGCAAGCACCACATCTTAATGAAGTCTGTG5540.1212882829826409No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGG5530.12106935106389967No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA5490.12019362338893473No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTA5480.1199746914701935No Hit
ATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGT5450.11931789571396982No Hit
CAGGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA5420.11866109995774614No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA5330.11669071268907508No Hit
GAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGG5320.11647178077033385No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC5280.11559605309536894No Hit
AATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAA5280.11559605309536894No Hit
CAGTTAAATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGT5270.11537712117662771No Hit
AGCAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACAG5260.11515818925788648No Hit
ATCATAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGC5230.11450139350166277No Hit
GGTGAACAGATGCGCAAACAGGTTCACGGTGCCTGGAATATTGGTGAACA5150.11274993815173295No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT5140.11253100623299173No Hit
CTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACA5130.1123120743142505No Hit
CTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTT5120.11209314239550927No Hit
TTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCT5070.11099848280180312No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG5030.11012275512683821No Hit
AGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTT4990.1092470274518733No Hit
GTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATT4970.10880916361439084No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAA4870.10661984442697854No Hit
ATTATGAACAGCCCACATGATTCGGAAAGCTGGGCCACCAATCTCATCAA4850.10618198058949609No Hit
GGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC4770.10443052523956625No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCGTGAACCTGTTTGCGCATCT4750.1039926614020838No Hit
GTAATGATCCATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT4740.10377372948334257No Hit
TAAGTGTACCATCTGTTTTTACGATATCATCTACAAAACAGCCGGCCCCT4730.10355479756460134No Hit
CAATAATAGCTCATACCTCCTAAGTAAAGTTGAGTCACATCTGTGACATC4720.10333586564586011No Hit
GTTCATAATGGTACTCGACCACCACTGATAAAAAATTTACCTAAGCCCAT4690.10267906988963643No Hit
GCATAACAGAATACATGTCTAACATGTGTCCTGTTAACTCATCATGTAGC4690.10267906988963643No Hit
GTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCA4660.10202227413341275No Hit
GGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT4620.10114654645844781No Hit
CTCCTGATTAGGATGTTTAGTAAGTGGGTAAGCATCTATAGCTAAAGACA4610.10092761453970658No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG4590.10048975070222413No Hit
GTTTAACTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTA4570.10005188686474167No Hit
GAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA4570.10005188686474167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA202.1293563E-6145.05
TTCCGGG202.1293563E-6145.07
TAACGGT100.0070896107145.04
GTAGCAG151.2295712E-4145.05
ATTCAAA100.0070896107145.06
CGTACCG100.0070896107145.05
CATAACG100.0070896107145.02
GCCGTGC100.0070896107145.08
GCGTAAT100.0070896107145.01
TATTCCG301.10074325E-7120.8333365
CTATCGA256.461085E-6116.04
TTATACG256.461085E-6116.02
ATTCCGG256.461085E-6116.06
ACCTATC256.461085E-6116.02
CGGTACC256.461085E-6116.07
CCTATCG256.461085E-6116.03
TATCGAG256.461085E-6116.05
ATCGAGC256.461085E-6116.06
TCCGGGC256.461085E-6116.08
TCTCTAA203.8646982E-4108.757
Produced by FastQC (version 0.11.5)