FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_32_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_32_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326644
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT11090.3395133539878277No Hit
AATTATGACCAACCAACTTTCGATCTCTTGTCTGAAGACATGCTTAACCC10190.31196042174354954No Hit
CCCTAATTATGACCAACCAACTTTCGATCTCTTGTCTGAAGACATGCTTA9340.28593820795728686No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG8120.24858867758170977No Hit
GCTTAACCCTAATTATGACCAACCAACTTTCGATCTCTTGTCTGAAGACA6870.2103207161313234No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG6430.19685039370078738No Hit
CTGATATAGTTGTCACTAGCTACATGTGATCATTCATCTAAATTCAAATG6290.1925643820183441No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT6030.18460464603666377No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC5590.17113432360612776No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5550.1699097488397154No Hit
ATAATACCCATAGCAAAAGGTAAAAAGGCATTTTCATACAAAAAAAAGAA5510.16868517407330305No Hit
ATCCAATATTGTAGCCACCAACCACATGTGACTATTGAGCACTTGAAATG5470.1674605993068907No Hit
CCTATATTGAGCACGTACTGAGAGCTAGGAACTGAGCTGGATCCCTCAAG5110.15643942640917943No Hit
AGCTACATGTGATCATTCATCTAAATTCAAATGAATTGCAGCTAAATCAC5090.15582713902597323No Hit
TATCTCTACTATCCAATATTGTAGCCACCAACCACATGTGACTATTGAGC4930.15092883996032377No Hit
CATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAA4860.14878583411910215No Hit
TGCTTAACCCTAATTATGACCAACCAACTTTCGATCTCTTGTCTGAAGAC4740.14511210981986505No Hit
GTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAA4550.1392953796794063No Hit
GGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGC4360.1334786495389476No Hit
GATTATAAGAGCCCACATGGAAATGGCTTGATCTAAAGCATTACCATAAT4300.13164178738932905No Hit
ATATTGAGCACGTACTGAGAGCTAGGAACTGAGCTGGATCCCTCAAGGAG4250.1301110689313136No Hit
CTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGA4210.12888649416490125No Hit
CTTGTTTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT4150.1270496320152827No Hit
CACCTATATTGAGCACGTACTGAGAGCTAGGAACTGAGCTGGATCCCTCA4010.12276362033283943No Hit
CATAAAAACAATACCTCTGGCCAAAAACATGACAGTTGTAACTACACCTG4010.12276362033283943No Hit
GGTGGAGGGAGAGCGGCAGAGGTAGTTTTCGTTTTTAATGGCTTCCTTTC3930.1203144708000147No Hit
GTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTT3910.11970218341680852No Hit
GTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCAT3870.11847760865039615No Hit
GGCATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAA3860.11817146495879308No Hit
CTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTT3830.11725303388398378No Hit
CACTAATGCTGAACTCAAGAGGAGACCTCTTCCTCAGTGTTTGACGCTTT3780.11572231542596834No Hit
ATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCT3720.11388545327634979No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG3640.11143630374352506No Hit
GTCTAACAACATCAAAAGGTGTAAATTCATCTTCTAATAAAGCACTACCC3600.11021172897711269No Hit
TTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA3580.10959944159390651No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC3570.10929329790230344No Hit
ATCTAAAGCATTACCATAATAAACTTTATAAACGAGTGTCAAGACATTCA3550.10868101051909723No Hit
ACATAACACAGTCTTTTAGCTTAAAACCAGACAAACTAGTATCAACCATA3550.10868101051909723No Hit
ATTCATAAGTGTCCACACTCTCCTAGCACCATCATCATACACAGTTCTTG3510.10745643575268488No Hit
ACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAG3500.10715029206108179No Hit
ATTGAGTACTCTGGACTAAAACTAAAAGTGAAGTCAAAATTGTGAGTAAC3340.10225199299543233No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAG100.0070886915145.04
GTTATAC100.0070886915145.01
CCGTGAC1450.090.000013
TGTAGAG1050.082.857142
GTAGAGC1100.079.0909043
GGCCGTG1750.074.571431
TTGGCCG300.001934571372.53
TAGAGCA1400.067.321434
CGTATGG350.00356435462.1428571
AAGCCGT350.00356435462.1428576
GTACTAA954.5474735E-1061.0526351
TTGATTA753.7533027E-758.0000042
GCCGTGA2300.056.7391322
TGACGTG400.006047252654.3757
GCCAGTA553.2157887E-452.7272728
TTTGAGG553.2157887E-452.7272723
GATTAAT752.5467789E-548.3333364
TCACGCA604.9401587E-448.3333322
TTAGTTG450.00963337248.3333323
ATGATAG951.9168856E-645.7894745
Produced by FastQC (version 0.11.5)