FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_31_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_31_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466752
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAAT263195.638754627725216No Hit
GGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTC229134.9090309200603315No Hit
CTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTG209804.494892362539421No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATA185833.9813434114904704No Hit
GTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCA97352.0856900452488687No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGT88111.8877262443438914No Hit
ATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCC82471.7668911970382557No Hit
ATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATT78611.6841920334567395No Hit
CCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACT75431.6160616344439875No Hit
TCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACA70141.5027252159605102No Hit
AGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCA56971.2205625257095847No Hit
CAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTG54781.1736425339366516No Hit
GTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA53511.146433223639106No Hit
GTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAA49091.0517362539421362No Hit
GTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGT47661.0210989990401755No Hit
ACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGT45330.9711795557383792No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTG44370.9506118881118882No Hit
TCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGT43650.9351861373920198No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTG43350.9287587412587412No Hit
GGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGA43040.9221170985876869No Hit
CTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTT37320.7995680789798436No Hit
CAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGT37220.7974256136020841No Hit
CATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCT35470.759932469491293No Hit
GCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC31560.6761620732208967No Hit
CTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGA29330.62838509529686No Hit
AACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAG28520.6110311257370081No Hit
CACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCA27420.5874640065816537No Hit
GAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACA27230.5833933223639106No Hit
ATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATT26040.5578979843685726No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCGCA23610.5058360756890169No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG23460.5026223776223776No Hit
GAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACC22600.484197175373646No Hit
TGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGC22290.4775555327025915No Hit
CCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGAC21890.46898567119155354No Hit
TTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTTGCG20600.4413478678184561No Hit
CCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGT19740.4229226655697244No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT19700.4220656794186206No Hit
CAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGAT19510.4179949952008775No Hit
GTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAA18940.40578294254764846No Hit
CAATCCACAATAGCGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAG17530.37557418072123955No Hit
ATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGG17300.3706465103523927No Hit
CAATTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGT16600.35564925270807624No Hit
GCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCA16330.3498645961881256No Hit
ATTTTTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGT15600.3342245989304813No Hit
GGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAAT14850.31815610859728505No Hit
CCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG14550.3117287124640066No Hit
GATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGC13860.29694570135746606No Hit
GTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA13060.2798059783353901No Hit
TGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTTT12290.26330899492664195No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT12080.25880981763334704No Hit
GGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCAC11690.250454202660085No Hit
ACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGT11680.2502399561223091No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC11670.25002570958453313No Hit
GCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAA11030.23631393116687235No Hit
CACCTGTTGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCG10940.23438571232688882No Hit
CAATTGGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAC10940.23438571232688882No Hit
TTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCA10930.23417146578911285No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT10800.2313862607980255No Hit
TTACAAAATTGACCAATATTCCAGGCACCACCATGGGCCTCATAGCACCA10800.2313862607980255No Hit
GGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGC10610.22731557658028245No Hit
CACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAG10480.22453037158919514No Hit
GGTTCATAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTC10320.22110242698477994No Hit
CACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCA10250.21960270122034828No Hit
CCAATATTCCGGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAAT9840.21081859317153437No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA9800.20996160702043054No Hit
ATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT9730.20846188125599893No Hit
CCAATACTCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAAT9210.19732106129164953No Hit
GCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCT9210.19732106129164953No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC9140.19582133552721787No Hit
CAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC9030.19346462361168243No Hit
CTGTGATCCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTG8920.191107911696147No Hit
CGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGATGCGCAA8780.1881084601672837No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT8330.17846736596736598No Hit
GGTTCACAGTAGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTC8270.17718188674071025No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG7900.16925476484300012No Hit
CTGTGAACCTATTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTG7790.16689805292746468No Hit
CCCAAAAATGGATCATTACAAAATTGACCAATATTCCAGGCACCACCATG7730.165612573700809No Hit
TAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGC7660.16411284793637734No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA7580.16239887563416974No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG7480.16025641025641024No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT7480.16025641025641024No Hit
CTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAAC7280.15597147950089127No Hit
CTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCACTGTGAACCTGTT7110.15232928835870013No Hit
AGCAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAA7040.1508295625942685No Hit
CTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTAC7010.15018682298094063No Hit
TTACAAAATTGACCAATATTCCAGGCACCACCATGGGCCTCATAGCACAT6570.14075997531879886No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCC6480.13883175647881532No Hit
GAATATCGGTGAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATA6480.13883175647881532No Hit
GAATATTGGTAAACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATA6380.1366892911010558No Hit
TGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC6330.13561805841217606No Hit
ATTTTTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAG6320.13540381187440012No Hit
ATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA6320.13540381187440012No Hit
CAATACCAATGTGCTATGAGGCCCATGGTGGTGCTATGAGGCCCATGGTG6260.13411833264774442No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACT6170.13219011380776086No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC6100.13069038804332922No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT5950.12747668997668998No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT5560.11912107500342796No Hit
TTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGC5360.11483614424790894No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG5340.11440765117235706No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC5280.11312217194570137No Hit
GTGCTATGAGGCCCATGGTGGTGCTATGAGGCCCATGGTGGTGCCTGGAA5260.11269367887014946No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC5070.10862299465240642No Hit
TCCAGGCACCACTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAG4890.10476655697243932No Hit
ACAGATGCGCAAACAGGTTCACAGTGGTGCCTGGAATATTGGTGAACAGA4890.10476655697243932No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA4860.10412381735911147No Hit
GTGCTATGAGGCCCAATGTGCTATGAGGCCCAATGTGCTATGAGGCCCAA4860.10412381735911147No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG100.0070896586145.05
GGTAAAA202.1294036E-6145.01
ACGCATT100.0070896586145.09
TGGACGC100.0070896586145.06
GATCCGT100.0070896586145.06
CCGGACA100.0070896586145.06
GATTTAC253.7083737E-8145.01
GCATTAA100.0070896586145.06
TACGAGG100.0070896586145.05
CTTGCAT100.0070896586145.05
CGAGGCC100.0070896586145.07
GGACGCA100.0070896586145.07
TGCTACG100.0070896586145.02
GACGCAT100.0070896586145.08
AGTAGAC100.0070896586145.06
TAGTGAG100.0070896586145.09
CTGGACG100.0070896586145.05
ATAGTGA151.2295887E-4144.999988
TCCGGGC1300.0139.423078
AGTAGTG700.0134.642858
Produced by FastQC (version 0.11.5)