FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_31_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_31_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476641
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGA81931.718903745166698No Hit
AATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTG69681.461896899343531No Hit
GCTTAACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAA64581.3548981308783759No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC64191.3467158721133936No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC63191.3257357214339514No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA60371.2665716965179243No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC59651.251465988028726No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA58131.219576158995974No Hit
TGCTTAACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTA41720.8752918863463277No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT38480.8073161981449352No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG37090.7781537887005104No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG33830.7097584974855289No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC32270.6770294624255991No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC31710.6652805780451115No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA31570.6623433569499896No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA31170.6539512966782127No Hit
GAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTT27940.5861854099836145No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA27460.5761149376574822No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA25700.539189872461664No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG25360.5320566212306537No Hit
TGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTG24210.5079294479492952No Hit
GATCACGCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACAC23570.49450215151445215No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA23070.4840120761747311No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA22770.4777180309708984No Hit
ATCACGCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACC21170.44414978988379095No Hit
ATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGT19960.4187638075616659No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA18850.39547584030748506No Hit
CTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT18200.3818387423658477No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT17400.3650546218222939No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA16910.35477434798936724No Hit
GGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGG15810.3316961822419809No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC14070.2951907200597515No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA13760.28868687334912435No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT13380.2807144160909364No Hit
CCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTA11750.24651677048344564No Hit
AGATCACGCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACA11650.2444187554155014No Hit
CTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGA10890.22847384089912534No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA10650.2234386047360592No Hit
ACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAAC10470.2196621776137596No Hit
ACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATG10180.2135779339167214No Hit
GGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGG10020.21022110980801062No Hit
CAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTA9760.20476627063135566No Hit
AATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTT9440.19805262241393418No Hit
ATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTC9420.1976330194003453No Hit
ATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGA9390.19700361487996207No Hit
ACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAA9280.19469579830522343No Hit
TGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCA9020.18924095912856845No Hit
GTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT8950.1877723485810075No Hit
ATGCTTAACCCTAATTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGT8500.17833128077525853No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC8340.17497445666654776No Hit
CTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTT8150.17098822803745375No Hit
ATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA7770.16301577077926574No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC7690.16133735872491037No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC7620.15986874817734942No Hit
TAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGAT7330.1537845044803112No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC7330.1537845044803112No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG7090.14874926831724505No Hit
ATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTC6640.13930820051149606No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC6460.13553177338919647No Hit
GCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGA6220.13049653722613036No Hit
GCTAGGGCCTGGAGATGCAGCCAGGGGAATGAGTTGGGAGCTAGGCCACC5980.12546130106306425No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT5920.1242024920222977No Hit
ACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCT5870.12315348448832558No Hit
AATTATGACTGCACCAAAAACAAAGTATCCTTCCCGCAAACTGCAAGAAT5710.11979666037961484No Hit
GTGCTGGGGTGCCTGCTTCCATGTGGGTGTCACCACAGAGTGGAGGCAGC5620.11790844681846505No Hit
GTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATAT5510.11560063024372641No Hit
ACGCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAA5330.11182420312142681No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT5250.11014579106707145No Hit
GGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCA5240.10993598956027702No Hit
GACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGA5200.10909678353309933No Hit
CATCAAGACAGCCCACACGTGGGTGGCCAGCTAAAAGCAGGTCTAGGGAA5190.10888698202630492No Hit
ACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTG5140.1078379744923328No Hit
GAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAA5130.1076281729855384No Hit
CTCCAGAGGTGGCTGGGCAGAGGTGGTGAGGCTCAGAGGCTTGGCTCAGG5110.10720856997194954No Hit
GTATAAGGAGCATGAGTTTCTTGCTAAGGCCTGGGCTGCCTGGGAGAAAG5020.10532035641079975No Hit
TTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTT4940.10364194435644436No Hit
GAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTG4930.10343214284964995No Hit
GTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAG4810.1009145247681169No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAT202.1294509E-6145.01
CCGATCT100.007089706145.09
GACCGAT100.007089706145.07
TTATACG100.007089706145.02
CGTACTA151.2296045E-4145.05
TCGACTC100.007089706145.0145
CCCGATT100.007089706145.08
GAGCGTG256.461376E-6116.07
ATCCTAA404.809408E-9108.756
GGACCGA203.864802E-4108.756
ATATTAC352.759516E-7103.571433
CTAATAA352.759516E-7103.571433
CGTGGAC2550.0102.3529363
ATCACGT301.5985961E-596.6666641
TGCTTAA7550.095.066221
CTCGTGG2850.091.578951
CCTAATA651.6370905E-1189.2307742
CATCCTA259.383713E-487.05
GGCCGTG2400.084.5833361
TACTAAT353.435427E-582.857142
Produced by FastQC (version 0.11.5)