FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_29_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_29_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681341
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTAC26500.38893887201856336No Hit
GTCTTATTACAGTAGGCTAAGATAAGTGCACAAAAGTTAGCAGCTTCACC18850.27666029198301584No Hit
GGTAATTACCAGTGTACTCACTAGCACAAGTAAATGTACCATGCTTAAGT17940.26330427788728406No Hit
GTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGT15750.2311617824261273No Hit
TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT15290.2244103906854277No Hit
CCCTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAA15130.2220620805147496No Hit
GTTTAAACACCGTGTAACTATGTTAGTAGTTGTACTAACAACTTTGTTAA15090.22147500297208006No Hit
GTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCA14960.2195670009584041No Hit
GCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTAT14300.20988022150435684No Hit
GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG14250.20914637457601992No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT14020.20577067870567015No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA13560.19901928696497057No Hit
CTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTT13420.19696451556562722No Hit
GTATAATGTCTCCTACAACTTCGGTAGTTTTCACATTACACTCAAGAACG13080.19197435645293617No Hit
GGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTT12660.18581004225490613No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC12360.18140696068488465No Hit
TCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATG12290.18037957498521298No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC12180.17876511174287177No Hit
AGTTCATACTGAGCAGGTGGTGCTGACATCATAACAAAAGGTGACTCCTG12170.1786183423572044No Hit
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC12040.17671034034352842No Hit
GTAATAAGCATCTTGTAGAGCAGGTGGATTAAACTTCAACTCTATTTGTT11710.17186695061650478No Hit
CCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAA11680.17142664245950265No Hit
GGTTCACCTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA11130.16335432624779664No Hit
GACTAGAATTGTCTACATAAGCAGCCATTAGATCTGTGTGGCCAACCTCT10440.15322723863674725No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT10040.14735646321005194No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC9620.14119214901202187No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG9600.14089861024068712No Hit
GGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCG9370.1375229143703373No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT9350.13722937559900256No Hit
CTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTT9200.13502783481399183No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA9130.13400044911432016No Hit
CGTATACACCAGGTATTTGGTTTATACGTGGCTTTATTAGTTGCATTGTT9060.13297306341464846No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG8750.12842321245895963No Hit
GGGTAAGGAAGGTACACATAATCATCACCCTGTTTAACTAGCATTGTATG8720.12798290430195747No Hit
GGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGA8610.12636844105961625No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG8580.12592813290261412No Hit
CAATACACCCAAAAATGGATCATTACAAATTGGTCCAGTAGGCAGCTCTC8440.12387336150327076No Hit
CTTAAGGGTTGTCTGCTGTTGTCCACAAGTTTTACACACCACGTTCAAGA8370.12284597580359909No Hit
ATGCTAGGGAGAGCTGCCTACTGGACCAATTTGTAATGATCCATTTTTGG8200.12035089624725358No Hit
CCTCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAGGTG7920.11624135344856688No Hit
GTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTA7830.11492042897756044No Hit
GTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTA7780.11418658204922351No Hit
GTTTATAATCAATAGCCACCACATCACCATTTAAGTCAGGGAAAAATGTA7750.11374627389222136No Hit
CTATTAACAGCAGCTAAACCATGAGTAGCAAGGGTTTTCAAACCTAATAC7680.11271888819254969No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG7610.11169150249287801No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT7590.11139796372154326No Hit
CTTATAACCAGTTAACTGGTTTAAATCATCAGCAAATTTGATATTATCAC7570.11110442495020849No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT7460.10948996170786728No Hit
CCAGGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAGGT7440.1091964229365325No Hit
GTTTTACACACCACGTTCAAGACTCTTTTGCAAGAATCTAAATTGGCATG7380.10831580662252822No Hit
AACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAA7350.10787549846552606No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT7310.10728842092285655No Hit
TTCTTAAATTGTTCATAAGAAAGTGTGCCCATGTACATAACAGCTTCTAC7260.10655457399451962No Hit
GTAAATGTACCATGCTTAAGTTCATACTGAGCAGGTGGTGCTGACATCAT7200.10567395768051535No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG7120.10449980259517627No Hit
AGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTG7080.10391272505250675No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG7080.10391272505250675No Hit
CGTACAAGGTATCTGAACACCTTTCTTAAATTGTTCATAAGAAAGTGTGC7040.1033256475098372No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG7020.10303210873850246No Hit
CCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATC6950.10200472303883076No Hit
GATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGA6920.10156441488182863No Hit
CCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAGGTG6880.10097733733915909No Hit
CTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTG6870.10083056795349171No Hit
CATTAGATCTGTGTGGCCAACCTCTTCTGTAATTTTTAAACTATTATTTG6820.10009672102515481No Hit
TACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAAC6820.10009672102515481No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTG151.2298347E-4145.07
ACCGGAC151.2298347E-4145.05
CCGGACC151.2298347E-4145.06
AATATGA151.2298347E-4145.03
GAGCCGT151.2298347E-4145.06
TTACCGG151.2298347E-4145.06
TACCGGT151.2298347E-4145.07
TACCGGA151.2298347E-4145.04
TGATCCG100.0070903706144.999985
AACGTTA100.0070903706144.999985
TATCAAT100.0070903706144.999987
GTACCGA100.0070903706144.999986
TTGTGAG100.0070903706144.999986
ACGTTAC100.0070903706144.999986
CCCTAGT100.0070903706144.999981
TATGGAA100.0070903706144.999988
TAGCGGC100.0070903706144.999984
TAACGTT100.0070903706144.999984
TCTAGCG100.0070903706144.999982
GCCGCGT100.0070903706144.999988
Produced by FastQC (version 0.11.5)