FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_28_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_28_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325722
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGCAAAGCACTCGTG20290.6229238430317878No Hit
GGGTCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCAC19940.6121784834920576No Hit
GCTAGACCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGGACA18460.566740963152627No Hit
CCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCAC14960.45928736775532514No Hit
GTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCATCAATTTC14830.4552962342119968No Hit
CATGTAGAGAGACCACATATAGAGAAAGATGCCAGAGGGATGCCAGCTGT14260.4377966486758647No Hit
GTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCAC14230.4368756178581735No Hit
GCTTTTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGAC13120.40279747760360063No Hit
GGGTACGAGTGCTTTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCAC12520.38437686124977744No Hit
GTTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGC12500.38376284070465No Hit
TCCCTATGGTGCTAGCTGTCCACGAGTGACACCACCATCAACCAACCAAC11550.3545968648110966No Hit
CTTGTGTTCTCAATTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCA10370.31836965264857764No Hit
GTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCATCAACCAA10190.31284346774243066No Hit
CCATAGGGAAGTCCAGCTCTGAAGACATGCTTAACCCTAATTATGTCACG9610.2950368719337349No Hit
GGTTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAG9050.2778442966701666No Hit
CTCGTACCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGGACA8740.2683269782206913No Hit
CCATAGGGAAGTCCAGCTATCCTGAAGCCAACATGTAGAGAGACCACATA8460.2597306905889071No Hit
GCTTAACCCTAATTATGTCACGAGTGACACCACCATCAATTTCCATTTGA7670.2354768790563732No Hit
CCATAGGGAAGTCCAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGT7660.23516986878380952No Hit
CTCGTGGACAGCTAGACCCAGGAGTCAAATGGAAATTGATGGTGGTGTCA7570.23240677633073603No Hit
CATATAGAGAAAGATGCCAGAGGGATGCCAGCTGTCCTAGCTGTCCACGA7330.22503852978920674No Hit
GTCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCA7190.2207403859733147No Hit
CTTGTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACA7170.22012636542818723No Hit
CTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGACCCAGGAGTCAA7170.22012636542818723No Hit
GGGTACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCATCAACCAA7050.21644224215742255No Hit
GTCCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACC7010.2152142010671677No Hit
CTCGTGGACAGCTAGCAAAGCACTCGTACCCAGGAGTCAAATGGAAATTG6800.20876698534332958No Hit
TCCCTATGGTGCTACAAGAGATCGAAAGTTGGTTGGTTGATGGTGGTGTC6730.20661791343538355No Hit
ACCCTAATTATGTCACGAGTGACACCACCATCAATTTCCATTTGACTCCT6520.20017069771154544No Hit
GTGCTGGACTTCCCTATGGTGCTAGCTGTCCACGAGTGACACCACCATCA6490.1992496668938543No Hit
GTCCAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGCTAG6350.1949515230779622No Hit
CTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTA6130.18819729708156036No Hit
CCCTAATTATGTCACGAGTGACACCACCATCAATTTCCATTTGACTCCTG6020.18482018408335943No Hit
CCATAGGGAAGTCCAGCCCTCAAATTCCAAGGTCCCACTAGCTGTCCACG5980.18359214299310456No Hit
GTCCACGAGTGACACCACCATCAATTGAGAACACAAGAGATCGAAAGTTG5950.18267111217541337No Hit
TCCCTATGGTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTG5940.18236410190284966No Hit
GTGCTAGCTGTCCACGAGTGACACCACCATCAACCAACCAACTTTCGATC5930.18205709163028594No Hit
TCCCTATGGTGCTGGACTTCCCTATGGTGCTAGCTGTCCACGAGTGACAC5790.17775894781439386No Hit
GTCCACGAGTGACACCACCATCAATTTCCATTTGACTCCTGGGTCTAGCT5740.17622389645157527No Hit
GCTAGGACAGCTGGCATCCCTCTGGCATCTTTCTCTATATGTGGTCTCTC5610.1722327629082469No Hit
ATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGC5550.1703907012728646No Hit
GCTAGCAAAGCACTCGTACCCAGGAGTCAAATGGAAATTGATGGTGGTGT5520.16946967045517344No Hit
CTTTGCTAGCTGTCCACGAGTGACACCACCATCAATTTCCATTTGACTCC5460.1676276088197911No Hit
GCTAGCAAAGCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGC5420.16639956772953624No Hit
GGGTCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGCTTTGCT5180.15903132118800695No Hit
GTCCACGAGTGACACCACCATCAATTTCCATTTGACTCCTGGGTACGAGT5150.1581102903703158No Hit
GTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCATCAATTGA5140.15780328009775207No Hit
CTTTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCA5080.15596121846236977No Hit
GCAACAGCCTGCCTTTTCTGGGTTGAGGCTGATGGTGGTGTCACTCGTGG4960.15227709519160512No Hit
TGCTTAACCCTAATTATGTCACGAGTGACACCACCATCAATTTCCATTTG4930.15135606437391394No Hit
GGTCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACC4860.1492069924659679No Hit
CTTGTGTTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGG4860.1492069924659679No Hit
GTTGATGGTGGTGTCACTCGTGGACAGCTAGCACCATAGGGAAGTCCAGC4740.14552286919520327No Hit
TTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACC4620.14183874592443863No Hit
CAGCTAGACCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGGA4560.13999668428905632No Hit
GTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACC4510.1384616329262377No Hit
CTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGCACCATAGGGAAG4480.13754060210854654No Hit
GCTCTGAAGACATGCTTAACCCTAATTATGTCACGAGTGACACCACCATC4470.13723359183598285No Hit
GGGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACC4400.13508451992803677No Hit
GCTAGAGGGTGTGGTCACACAGGGAAGAGCCACTGGAGAACAAAGGCTGG4390.13477750965547305No Hit
GTGTTCTCAATTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTC4380.13447049938290936No Hit
GTCCAGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACC4320.13262843774752703No Hit
GTCAAATGGAAATTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCAC4300.1320144172023996No Hit
GTGCTACAAGAGATCGAAAGTTGGTTGGTTGATGGTGGTGTCACTCGTGG4270.13109338638470844No Hit
TAGCAACAGCCTGCCTTTTCTGGGTTGAGGCTGATGGTGGTGTCACTCGT4250.130479365839581No Hit
TGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTA4170.12802328365907123No Hit
GCTAGCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGGACAGC4110.12618122202368892No Hit
CTTTGCTAGCTGTCCACGAGTGACACCACCATCAACCAACCAACTTTCGA4080.12526019120599777No Hit
GCTAGCCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGGACAG4010.12311111929805173No Hit
CTCGTGGACAGCTAGCCCAGGAGTCAAATGGAAATTGATGGTGGTGTCAC3990.12249709875292428No Hit
GTGCTTTGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACA3950.12126905766266939No Hit
GTCCACGAGTGACACCACCATCAACCAACCAACTTTCGATCTCTTGTCAC3930.12065503711754197No Hit
GCTAGCAAAGCACTCGTGGACAGCTAGACCCAGGAGTCAAATGGAAATTG3910.12004101657241452No Hit
TTTCTGGGTTGAGGCTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGC3870.11881297548215963No Hit
TCCCTATGGTGCTACCAACCAACTTTCGATCTCTTGTGTTCTCAATTGAT3870.11881297548215963No Hit
CAATTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACA3850.11819895493703218No Hit
CTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGCAAAGCACTCGTA3760.1154358624839587No Hit
GTCCACGAGTGACACCACCATCAACCAACCAACTTTCGATCTCTTGTGTT3700.1135938008485764No Hit
CCAGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCAC3670.11267277003088523No Hit
GGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGCAAAGC3660.1123657597583215No Hit
GTGGTGTCACTCGTGGACAGCTAGCAAAGCACTCGTGGACAGCTAGCAAA3630.11144472894063036No Hit
TCCCTATGGTGCTGGACTTCCCTATGGTGCTACCAACCAACTTTCGATCT3610.11083070839550291No Hit
GGTGCTAGCTGTCCACGAGTGACACCACCATCAACCAACCAACTTTCGAT3580.10990967757781174No Hit
GGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCACCA3570.10960266730524802No Hit
GGTTGATGGTGGTGTCACTCGTGGACAGCTAGCACCATAGGGAAGTCCAG3480.10683957485217455No Hit
GCTAGACCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACTCGTGACAT3480.10683957485217455No Hit
CTCGTGGACAGCTAGCCAGGAGTCAAATGGAAATTGATGGTGGTGTCACT3440.10561153376191967No Hit
CTCTATATGTGGTCTCTCTACATGTTGGCTTCAGGATAGCTGGACTTCCC3350.1028484413088462No Hit
CCTGGCTAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGACACCA3280.10069936940090016No Hit
CTCTTGTGTTCTCAATTGATGGTGGTGTCACTCGTGGACAGCTAGCAAAG3260.10008534885577272No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTT151.2292499E-4145.01
GGTCTTG151.2292499E-4145.02
GTACTAT151.2292499E-4145.01
TAAAAAC151.2292499E-4145.03
CTTATCC151.2292499E-4145.03
TATTTAT253.705827E-8145.05
GTGGTAT151.2292499E-4145.06
AGATCAC151.2292499E-4145.01
TACTATT151.2292499E-4145.02
ACTATTT151.2292499E-4145.03
GCTTATC151.2292499E-4145.02
ATTTATA151.2292499E-4145.06
CTATTTA253.705827E-8145.04
ACTAAGC100.0070886835144.999982
CCTACGG202.1284286E-6144.999983
GTACCTA100.0070886835144.999982
CAATTAA100.0070886835144.999986
TCAACCG100.0070886835144.999985
ATAGCGC100.0070886835144.999986
TAACTAG100.0070886835144.999983
Produced by FastQC (version 0.11.5)