FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_27_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_27_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381169
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGG86712.27484396684935No Hit
CCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCACCAATATT53121.3936075599012512No Hit
CCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCAC52491.377079458193085No Hit
GGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGG52151.3681595302870906No Hit
ATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCA42431.113154532503955No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTG35420.9292466071480104No Hit
GCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCA31760.8332262067481878No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGGTAGGCAGCTCTCCCTA28190.739566963735246No Hit
TTACAAAATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAG24380.6396113010239552No Hit
CCCTGTTCACCAATATTCCAGGCACCCTGTTTGCGCATCTGTTCACCAAT22920.601308081192332No Hit
AAATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC19970.5239145890667919No Hit
GGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGGTGCCT19940.5231275366044983No Hit
GTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCACCA19880.5215534316799111No Hit
ATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTTGGGTGT18580.4874478249805204No Hit
CCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTGCGCATCTGT18350.48141375610293596No Hit
GGGTGCCTGGAATATTGGTGAACAGGGTGCCTGGAATATTGGTGAACAGA17770.4661974084985925No Hit
GGTTCAGGGCAATTTTGTAATGATCCATTTTTGGGTGTGGGTGCCTGGAA15900.417137805015623No Hit
CCCTAGCATTGGTGCCTGGACACATTGAGCCCACAATACACCCAAAAATG15690.41162843777956765No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGGGCAATTTTGTAATGA15640.4103166836757449No Hit
TTGGTGAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGTGAA15500.4066437721850413No Hit
CCGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCAC14710.3859180573446424No Hit
CCCTAGCATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTG14050.3686029031741826No Hit
CCCACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTGCGC13630.35758416870207177No Hit
CCCCTATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA13340.34997599489990006No Hit
ATTGGTAGGCAGCTCTCCCTAGCATTGAGCCCACAATACACCCAAAAATG12810.3360714013993793No Hit
ATTGGTAGGCAGCTCTCCCTAGCATTGGTGCCTGGACACATTGAGCCCAC12520.32846322759720753No Hit
CCTGTATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA11880.3116727750682768No Hit
ATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCA11730.30773751275680866No Hit
GTGCTATGAGGCCCAGAAGGTGCCTGGAATATTGGTGAACAGGTGCCTGG11670.3061634078322214No Hit
CAATACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTGCG11510.30196579469998874No Hit
GGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGG11340.2975058307469915No Hit
GGTTCAGGGCAATTTTGTAATGATCCATTTTTGGGTGTCTGGACACATTG11330.29724347992622696No Hit
CAGGGTGCCTGGAATATTGGTGAACAGGGTGCCTGGAATATTGGTGAACA11110.29147176186940704No Hit
CCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCTGTTC11080.2906847094071134No Hit
CAATGTGCTATGACCAACGGTGCCTGGAATATTGGTGAACAGATGCGCAA10840.28438828970876434No Hit
CAGGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGT10600.27809187001041535No Hit
GCATTGGTGCCTGGACACATTGAGCCCACAATACACCCAAAAATGGATCA10370.27205780113283085No Hit
GGTGAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGTGAACA10290.2699589945667145No Hit
ATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTG10150.2662860830760109No Hit
CAATTGGTGCCTGGAATATTGGTGCCTGGAATATTGGTGAACAGATGCGC10050.2636625748683655No Hit
CCCCCATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA9700.2544802961416065No Hit
CCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTGTTCA9160.24031335182032118No Hit
GAATATTGGTGAACAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACA8900.23349223048044304No Hit
ATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCT8890.2332298796596785No Hit
GTGGGCCCAATGTGCTATGAGGCCCAGAAGGTGCCTGGAATATTGGTGAA8700.2282452140651522No Hit
CAGGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA8630.2264087583198004No Hit
CAATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTTGGGT8490.2227358468290968No Hit
TCTGTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCA8430.22116174190450955No Hit
CCAATATTCCAGGCACCCTGTTCACCAATATTCCAGGCACCCTGTTTGCG8270.21696412877227686No Hit
CCCCTATTGTGGGCTCAATGTGTCCAGGCACCACACCCAAAAATGGATCA8040.21093005989469238No Hit
CAATAGGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGG8020.2104053582531633No Hit
GGTTCAGGGCAATTTTGTAATGATCCATTTTTGGGTGTGGTGCCTGGACA7910.20751949922475332No Hit
GAATATTGGTGAACAGATGCGCAAACAGGGTGCCTGGAATATTGGTGAAC7740.2030595352717561No Hit
CCAATATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA7620.1999113254225816No Hit
GCATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTGGTAGG7590.19912427296028798No Hit
GCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCTGTT7550.1980748696772298No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGGGCAATTTTGTAATGATCCAT7370.193352554903468No Hit
CTGTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCAC7200.18889259095047078No Hit
CCAATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTTGGG7110.18653143356358992No Hit
CAATAGGGGTGCCTGGAATATTGGTGCCTGGAATATTGGTGAACAGATGC7100.18626908274282536No Hit
CAATGGGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGG7060.1852196794597672No Hit
CCAATATTCCAGGCACCTGTTCACCAATATTCCAGGCACCTTCTGGGCCT6990.1833832237144154No Hit
GCCCAGAAGGTGCCTGGAATATTGGTGAACAGGTGCCTGGAATATTGGTG6810.17866090894065362No Hit
CCTGTATTGTGGGCTCAATGTGTCCAGGCACCACACCCAAAAATGGATCA6800.17839855811988908No Hit
ATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGG6720.17629975155377273No Hit
GGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGCCTG6620.1736762433461273No Hit
CATCTGTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTT6590.17288919088383367No Hit
GGTTCACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGG6350.16659277118548466No Hit
CCCTAGCATTGTGGGCCCAATGTGCTATGAGGCCCAGAAGGTGCCTGGAA6320.16580571872319103No Hit
CCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACACC6290.1650186662608974No Hit
CCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCAC6290.1650186662608974No Hit
CCACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTGCGCA6220.1631822105155456No Hit
CAATACAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGG6210.16291985969478104No Hit
CAATCGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGGGT6180.1621328072324874No Hit
GTTCAGGGCAATTTTGTAATGATCCATTTTTGGGTGTCTGGACACATTGA6150.1613457547701938No Hit
CCAATATTCCAGGCACCCTGTTTGCGCATCTGTTCACCAATATTCCAGGC5940.1558363875341384No Hit
CAATTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGGGT5920.15531168589260932No Hit
CCCCCATTGTGGGCTCAATGTGTCCAGGCACCACACCCAAAAATGGATCA5770.15137642358114117No Hit
GCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTGTTC5730.150327020298083No Hit
CATTGAGCCCACAATACACCCAAAAATGGATCATTACAAAATTGGTAGGC5620.14744116126967302No Hit
ATTTTGTAATGATCCATTTTTGGGTGTGGGTGCCTGGAATATTGGTGAAC5590.1466541088073794No Hit
GAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGTGAACAGAT5260.13799653172214948No Hit
CCAATATTCCAGGCACCCACACCCAAAAATGGATCATTACAAAATTGCCC5200.13642242679756225No Hit
GGGCCCAATGTGCTATGAGGCCCAGAAGGTGCCTGGAATATTGGTGAACA5190.1361600759767977No Hit
GTCCAGACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTG5130.13458597105221043No Hit
GGCCCAATGTGCTATGAGGCCCAGAAGGTGCCTGGAATATTGGTGAACAG5040.13222481366532954No Hit
GGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACG4990.13091305956150684No Hit
GCCCACAATACACCCAAAAATGGATCATTACAAAATTGGTAGGCAGCTCT4980.1306507087407423No Hit
CCCAAAAATGGATCATTACAAAATTGGTAGGCAGCTCTCCCTAGCATTGT4980.1306507087407423No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGC4950.12986365627844867No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCT4890.12828955135386141No Hit
CCCATATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACA4850.12724014807080325No Hit
CAATCCCAATGTGTGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAG4820.12645309560850962No Hit
CACCAATATTCCAGGCACCCACCACCTGTTTGCGCATCTGTTCACCAATA4790.125666043146216No Hit
GCCTACCAATTTTGTAATGATCCATTTTTGGGTGTATTGTGGGCTCAATG4780.12540369232545143No Hit
CCCAATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTTGG4750.12461663986315782No Hit
GGTGAACAGATGCGCAAACAGGTTCAGGGCAATTTTGTAATGATCCATTT4680.122780184117806No Hit
CAATCAATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTT4620.12120607919321877No Hit
GCCTACCAATTTTGTAATGATCCATTTTTGGGTGTCTGGACACATTGAGC4590.12041902673092512No Hit
CACCAATGCTAGGGAGAGCTGCCTACCAATTTTGTAATGATCCATTTTTG4540.11910727262710241No Hit
CAATATTGGTGCCTGGAATATTGGTGCCTGGAATATTGGTGAACAGATGC4520.11858257098557334No Hit
CCCAAAAATGGATCATTACAAAATTGGTAGGCAGCTCTCCCTAGCATTGG4520.11858257098557334No Hit
GTGAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGTGAACAG4500.11805786934404425No Hit
GGCACCACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCTGTTTG4480.11753316770251516No Hit
CAATATTCCAGGCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCAC4470.11727081688175062No Hit
ATATTGGTGAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGT4450.11674611524022152No Hit
CCTACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCAC4430.11622141359869244No Hit
GTTCAGGGCAATTTTGTAATGATCCATTTTTGGGTGTGGGTGCCTGGAAT4400.11543436113639882No Hit
CCCACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAATATT4390.11517201031563427No Hit
CCAATATTGTGGGCTCAATGTGTCCAGGCACCACACCCAAAAATGGATCA4370.11464730867410519No Hit
GATGCGCAAACAGGTGGTGGGTGCCTGGAATATTGGTGAACAGATGCGCA4290.11254850210798886No Hit
CCCAAAAATGGATCATTACAAAATTGGTAGGCAGCTCTCCCTAGCATTGA4200.11018734472110796No Hit
CCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCACCTGTTTG4180.10966264307957886No Hit
CCCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCACCTGTTT4180.10966264307957886No Hit
CCACCCATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTAC4170.10940029225881434No Hit
CCTTCTGGGCCTCATAGCACATTGGGCCCACAATACACCCAAAAATGGAT4170.10940029225881434No Hit
CACCACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCACCACCT4160.1091379414380498No Hit
CACAATACACCCAAAAATGGATCATTACAAAATTGGTAGGCAGCTCTCCC4150.10887559061728526No Hit
CAATACCAATGTGCTATGAGGCCCAGAAGGTGCCTGGAATATTGGTGAAC4110.10782618733422708No Hit
CCCAAAAATGGATCATTACAAAATTGACACCCAAAAATGGATCATTACAA4050.10625208240963983No Hit
GAATATTGGTGAACAGGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC4020.10546502994734619No Hit
CCAATTGTGGGCTCAATGTGTCCAGACACCCAAAAATGGATCATTACAAA4010.10520267912658166No Hit
GTCCAGGCACCACACCCAAAAATGGATCATTACAAAATTGCCCTGAACCT3990.10467797748505256No Hit
ATTGCCCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCAA3990.10467797748505256No Hit
CAATACGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGGG3960.10389092502275893No Hit
GTTCAGGGCAATTTTGTAATGATCCATTTTTGGGTGTGGTGCCTGGACAC3950.10362857420199438No Hit
ATTGACACCCAAAAATGGATCATTACAAAATTGGTAGGCAGCTCTCCCTA3910.10257917091893623No Hit
CTGGAATATTGGTGAACAGATGCGCAAACAGGTGGTGGGTGCCTGGAATA3890.10205446927740713No Hit
ATTGGTAGGCAGCTCTCCCTAGCATTGGTGCCTGGAATATTGGTGAACAG3880.10179211845664259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGAT306.493792E-10145.01
CTGAGCC600.0145.07
GCGATTT306.493792E-10145.09
GTCCGGA100.0070891525145.07
GCGGCTC151.2294129E-4145.09
ATGGTCG100.0070891525145.0145
CATTCGT100.0070891525145.0145
ACCCGAA306.493792E-10145.07
TCCGGAC100.0070891525145.08
GAACCTA151.2294129E-4145.05
CCCGAAA306.493792E-10145.08
TAATAAT100.0070891525145.07
CTTCGGG100.0070891525145.0145
GTTGGCA400.0145.05
TCTCTAG100.0070891525145.05
CGTGATC306.493792E-10145.02
AGCAACC100.0070891525145.0145
AATGTAC100.0070891525145.06
ACGGTAC100.0070891525145.06
GTAATAA100.0070891525145.06
Produced by FastQC (version 0.11.5)