FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_27_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_27_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683686
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGG454026.640767837867092No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGA432626.3277586494384845No Hit
GGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCAC249883.654894205819631No Hit
GTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCC238303.48551820572602No Hit
GTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTG191232.797044257158988No Hit
GTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAG177202.5918330929695794No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGAC172032.51621358342865No Hit
CAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGAC167652.4521490859839163No Hit
AGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGC92751.356616926483795No Hit
GCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCT91291.3352620940022173No Hit
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCT88801.298841866002814No Hit
GTCCAGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCA83921.22746406976302No Hit
CCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATA82361.20464657752243No Hit
GCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTT74911.0956784254760226No Hit
GCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGG68190.9973876896704043No Hit
CTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGC62000.9068490505875505No Hit
GCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAG56500.8264027638418806No Hit
GTGCTAGCCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATGGTGCT48300.706464663602882No Hit
GGCTAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGCTA47750.6984200349283151No Hit
GTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTA46050.673554819025108No Hit
CAGCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCT45540.666095254254146No Hit
ATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAG45320.6628774027843191No Hit
GGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCC40120.5868190953156859No Hit
GTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTAGCTGTCCA35900.525094853485372No Hit
CAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCT32790.4796061349800932No Hit
GAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAG31890.4664421971489836No Hit
CTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTAT30990.45327825931787397No Hit
GGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCC30380.44435603478789976No Hit
CTCGTGGACAGCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGT25320.3703454509818835No Hit
GTCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTG23740.34723542678949104No Hit
CCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGT23490.34357877739196063No Hit
CGATAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCAC21590.31578824197072924No Hit
GCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAG21540.31505691209122316No Hit
GTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGG20980.306866017440755No Hit
GCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCAC19890.29092302606752224No Hit
GCTTTGGCTAGCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTT19460.28463358910376985No Hit
GCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTG19270.28185453556164675No Hit
CACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGC18660.2729323110316724No Hit
GGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGC18250.2669354060197225No Hit
GTGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGAC17800.2603534371041677No Hit
TCCCTATGGTGCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGT17520.2562579897789336No Hit
AGCCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATGGTGCTAGCCA16930.24762829720076174No Hit
CTTGTGATGGTGGTGTCACTCGTGGACAGCTGGACTTCCCTATGGTGCTA16790.2455805735381447No Hit
CGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTT16690.2441179137791325No Hit
CGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAG16530.24177765816471306No Hit
CCAGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGC16400.2398762004779972No Hit
GTGCTAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCT16070.235049423273257No Hit
AAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGC15930.23300169961064No Hit
GTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGC15900.2325629016829363No Hit
TCCCTATGGTACTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGG15390.2251033369119742No Hit
AGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCT15250.22305561324935716No Hit
CCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTG15190.22217801739394988No Hit
CCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCAC15180.22203175141804862No Hit
CCATAGGGAAGTCCAGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGT14870.21749750616511088No Hit
CCATAGGGAAGTCCAGCACCATAGGGAAGTCCAGCACCATAGGGAAGTCC14380.21033047334595123No Hit
TCCCTACGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGG14080.20594249406891466No Hit
CAGCTAGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGAC13960.20418730235810004No Hit
CCATAGGGAAATCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGA13880.20301717455089033No Hit
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCAAAGCACTCGT13530.19789786539434767No Hit
CAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCA13500.19745906746664404No Hit
CACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGG13280.19424121599681726No Hit
GCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCTGG12940.18926817281617586No Hit
GCTTAACCCTAATTATGGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCAC12880.18839057696076855No Hit
GTGCTGGACTTCCCTATGGTGCTGGACTTCCCTATGGTGCTGGACTTCCC12820.18751298110536124No Hit
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGACTTCCCTATGG12260.17932208645489303No Hit
GGCTAGCACCATAGGGAAGTCCAGCTAGCACCATAGGGAAGTCCAGCTGT12030.17595796900916502No Hit
TCCCTATGGTGCTGGACTTCCCTATGGTGCTGGACTTCCCTATGGTGCTG11870.17361771339474552No Hit
GTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTAGCACCATA11850.17332518144294312No Hit
CTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCTGGACTTCCCTATG11770.17215505363573336No Hit
TTGTCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGC11520.16849840423820292No Hit
GTGCTATCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCG11470.16776707435869684No Hit
GTGCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCT10980.16060004153953716No Hit
GTCCAGCACCATAGGGAAGTCCAGCACCATAGGGAAGTCCAGCACCATAG10740.15708965811790793No Hit
GCACCATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGCTAGCACC10530.15401807262398237No Hit
GTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCT10510.15372554067217992No Hit
GCTTAACCCTAATTATGATGCTAGCTGTCCACGAGTGCTTTGGCTAGCAC10340.15123901908185922No Hit
GGCTAGTACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCAC10290.15050768920235313No Hit
GCTAGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGACTT9820.1436331883349959No Hit
CCCTAATTATGATGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGG9690.14173173064828004No Hit
TAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGGAC9680.14158546467237884No Hit
TAATTATGGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAA9150.13383336794961428No Hit
CCATAGGGAAGTCCAGCTTAGCACCATAGGTAGCACCATAGGGAAGTCCA8960.13105431440749116No Hit
GCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCA8900.13017671855208385No Hit
CCCTAATTATGGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGG8570.12534994134734367No Hit
AAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCG8540.12491114341964002No Hit
GGCTAGCACCGTAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCAC8480.12403354756423271No Hit
CTTGTTGATGGTGGTGTCACTCGTGGACAGCTGGACTTCCCTATGGTGCT8450.12359474963652906No Hit
TCCCTATGGTGCTACCTATGGTGCTAAGCTGGACTTCCCTATGGTGCTAG8350.12213208987751688No Hit
CTTGTGTGATGGTGGTGTCACTCGTGGACAGCTGGACTTCCCTATGGTGC8140.1190605043835913No Hit
AGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCAC8130.11891423840769007No Hit
AGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTC8030.1174515786486779No Hit
ATAGGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGG8030.1174515786486779No Hit
TGCTTAACCCTAATTATGGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCA8000.11701278072097424No Hit
CCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGCTAGCTGTCCACGAGT7980.11672024876917182No Hit
GTGCTAGCCAGAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCC7860.1149650570583572No Hit
CCATAGGGAAGTCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGA7850.11481879108245598No Hit
CTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCTGGACTTCCCTATGGT7850.11481879108245598No Hit
CCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACGAGTG7840.11467252510655476No Hit
CTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTGTCCACG7820.11437999315475232No Hit
GCTAGTGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCT7720.11291733339574014No Hit
CAGCTGGACTTCCCTATGGTGCTAGCTGGACTTCCCTATGGTGCTAGCCA7690.1124785354680365No Hit
CAGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGAC7640.1117472055885304No Hit
GTCCAGTTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCTG7570.1107233437572219No Hit
GGGTGCTGGACTTCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTGG7470.10926068399820969No Hit
CTTGTGTTCTCAATTGATGGTGGTGTCACTCGTGGACAGCTGGACTTCCC7410.1083830881428024No Hit
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGCAAGGCACTACC7380.10794429021509874No Hit
GGGAAGTCCAGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAA7320.10706669435969145No Hit
GTCCACGAGTGCTTTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATA7280.10648163045608655No Hit
GTCCAGCACCATAGGGAAGTCCAGCTGTCCACGAGTGCTTTGGCTAGCAC7270.10633536448018535No Hit
GTCCAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTCCAGCAC7060.10326377898625977No Hit
TCCCTATGGTGCTATCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAA6990.10223991715495125No Hit
GCTAGCCAAAGCACTCGTGGACAGCTGGACTTCCCTATGGTGCTAGCCAA6970.10194738520314882No Hit
GTCCAGCACTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAGGGAAGTC6970.10194738520314882No Hit
ACCCTAATTATGGTGCTAGCTGTCCACGAGTGCTTTGGCTAGCACCATAG6960.1018011192272476No Hit
TCCCTATGGTGCTAGCCAAAGCACTCGTGGACAGCTAGACAAAGCACTCG6930.10136232129954394No Hit
CCATAGGGAAGTCCAGCTGTCCACGATAGCACCATAGGGAAGTCCAGCTG6870.10048472544413663No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT151.2298366E-4145.04
CTTAATC151.2298366E-4145.02
TTAATCC151.2298366E-4145.03
TGCTTAT151.2298366E-4145.01
TGCTGAT100.0070903758145.05
GCTTAAT151.2298366E-4145.01
GTACTGG100.0070903758145.09
GTACTAT100.0070903758145.09
AATTATA202.1301203E-6145.05
AGTAGTA100.0070903758145.02
CAGTAGT100.0070903758145.01
CAGTACT100.0070903758145.04
ATGCAAA306.493792E-10145.01
GTAGTAC100.0070903758145.03
GGTACTA2550.0133.627448
GTACTAG2500.0130.59
GGACAAC1150.0126.086966
ATGGTAC2850.0124.6491246
TGCAAAG351.900844E-9124.285712
TGGTACT2900.0122.57
Produced by FastQC (version 0.11.5)