FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_19_D.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_19_D.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379078
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG34910.9209186499876015No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA22140.5840486654461615No Hit
GACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAG21690.5721777576118899No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG20910.5516015173658192No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG19460.5133508143442774No Hit
GTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAA17150.45241348746168336No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA15640.4125799967289054No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC14680.387255393349126No Hit
ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAAT14420.3803966466004358No Hit
GTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTTTAGTG13860.3656239612955645No Hit
CGTTTATATAGCCCATCTGCCTTGTGTGGTCTGTATGAGTTTAGGCCTGA13550.35744622478751076No Hit
GTATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTTGCAA13240.349268488279457No Hit
ATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCT12720.3355509947820765No Hit
GCTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAATCTGCAGCAG12580.3318578234558587No Hit
AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC12220.3223610971884414No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC12020.3170851381509874No Hit
GTGTTACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTA10620.2801534248888092No Hit
GTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCTCAG10400.27434986994760974No Hit
ATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGG10390.27408607199573704No Hit
CCATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCA9880.26063237645022924No Hit
AAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAAC9790.2582581948833749No Hit
AGTCAGCACTGCTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAA9610.2535098317496663No Hit
CCCTAATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAAC9490.25034425632719387No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT9470.24981666042344844No Hit
ACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAA9370.24717868090472148No Hit
GTTTTGTATGCGTCAATATGCTTATTCAGCAAAATGACTTGATCTTTGAA9090.23979233825228582No Hit
GTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCT9080.2395285403004131No Hit
GCCTTGTGTGGTCTGTATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTC8940.2358353689741953No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT8850.23346118740734098No Hit
GTATACACTATGCGAGCAGAAGGGTAGTAGAGAGCTAGGCCAATAGCAAA8830.23293359150359555No Hit
CAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAA8430.22238167342868748No Hit
ATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGC8240.21736951234310617No Hit
ACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGAC8200.21631432053561536No Hit
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC8170.21552292667999726No Hit
GTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTT7900.2084003819794343No Hit
CTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTC7860.20734519017194353No Hit
GTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTTGCAATTGTTT7540.19890365571201704No Hit
ACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCA7490.19758466595265353No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA7290.1923087069151995No Hit
GACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCC7290.1923087069151995No Hit
GTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGC7080.18676894992587278No Hit
GTTACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGA7030.18544996016650928No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG6960.18360337450340036No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG6870.18122919293654605No Hit
GTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCA6850.18070159703280064No Hit
CATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGAC6810.17964640522530984No Hit
GAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAA6800.17938260727343713No Hit
TTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAA6600.1741066482359831No Hit
GCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAA6590.1738428502841104No Hit
TCAATATGCTTATTCAGCAAAATGACTTGATCTTTGAAATTTGGATCTTT6550.1727876584766196No Hit
CCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACG6510.17173246666912878No Hit
ATTCAAGGCTCCCTCAGTTGCAACCCATATGATGCCGTCTTTGTTAGCAC6490.17120487076538338No Hit
AGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTG6450.17014967895789257No Hit
GTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCT6240.16460992196856583No Hit
CACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCA6210.16381852811294773No Hit
TCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATACAGACCACAC5920.1561683875086394No Hit
GTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTG5820.15353040798991238No Hit
CTCACATAGTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTATGC5790.15273901413429425No Hit
CTCCTATTTGTACTTTACTGTTTTTAGTTACACGATAACCAGTAAAGACA5740.15142002437493074No Hit
ATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACAT5670.14957343871182185No Hit
ACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGAT5640.14878204485620372No Hit
GATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGC5580.1471992571449675No Hit
CACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATC5570.14693545919309484No Hit
GCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACA5570.14693545919309484No Hit
CTCCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGG5540.1461440653374767No Hit
GGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCAT5490.1448250755781132No Hit
GTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTATATATC5460.14403368172249512No Hit
CTGTATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTTGC5380.14192329810751347No Hit
TACTTGTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTAT5370.1416595001556408No Hit
GTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGCAAAAAGCTTG5350.1411319042518954No Hit
ATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTGCCTTG5310.1400767124444046No Hit
GCACAAGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATAT5310.1400767124444046No Hit
GTTGCGACTACGTGATGAGGAACGAGAAGAGGCTTGACTGCCGCCTCTGC5280.13928531858878646No Hit
GAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAG5250.13849392473316838No Hit
ACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGT5230.13796632882942297No Hit
CTTCTGCGTAGAAGCCTTTTGGCAATGTTGTTCCTTGAGGAAGTTGTAGC5190.13691113702193217No Hit
GTTCCTTGTCTGATTAGTTCCTGGTCCCCAAAATTTCCTTGGGTTTGTTC5160.13611974316631406No Hit
GATTAGTTCCTGGTCCCCAAAATTTCCTTGGGTTTGTTCTGGACCACGTC5160.13611974316631406No Hit
GAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAAC5080.13400935955133245No Hit
AACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACC5060.13348176364758704No Hit
AGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTC5010.13216277388822353No Hit
GAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACA4910.12952479436949652No Hit
CCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGA4900.1292609964176238No Hit
CTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAATG4880.1287334005138784No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA4880.1287334005138784No Hit
ACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGC4880.1287334005138784No Hit
CTGTATACACTATGCGAGCAGAAGGGTAGTAGAGAGCTAGGCCAATAGCA4870.1284696025620057No Hit
GTGCTGACTCAACTCAGGCCTAAACTCATACAGACCACACAAGGCAGATG4860.128205804610133No Hit
ACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTGCC4820.1271506128026422No Hit
TGCTTATTCAGCAAAATGACTTGATCTTTGAAATTTGGATCTTTGTCATC4820.1271506128026422No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG4780.1260954209951514No Hit
ATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTATATATCGTAAA4680.12345744147642437No Hit
ACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAA4650.12266604762080627No Hit
CCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTC4620.12187465376518818No Hit
TGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAA4600.12134705786144276No Hit
GTAGCAATACCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGT4600.12134705786144276No Hit
GAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCG4600.12134705786144276No Hit
AGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAG4550.12002806810207925No Hit
GCATTACTGTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTG4500.11870907834271574No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC4490.11844528039084304No Hit
GTATGTGATGTCAGCACAAAATAATCACCAACATTTAATTTGTAAGTTGT4480.11818148243897034No Hit
GTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGA4460.11765388653522495No Hit
CTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAAT4450.11739008858335225No Hit
AGTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATA4410.11633489677586144No Hit
TTACCAGACATTTTGCTCTCAAGCTGGTTCAATCTGTCAAGCAGCAGCAA4390.11580730087211602No Hit
CCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATT4390.11580730087211602No Hit
GTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATT4380.11554350292024332No Hit
AACTCATACAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTT4370.11527970496837063No Hit
GTACAGGTGTTAGCTAAAATGTAATCACCAGCATTTGTCCAGTCACATGT4370.11527970496837063No Hit
TGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCT4350.11475210906462523No Hit
GGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCC4340.11448831111275251No Hit
GCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTT4340.11448831111275251No Hit
GTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTATGCGAGCAGAA4340.11448831111275251No Hit
GTGTAGGTGCACTTAATGGCATTACTGTATGTGATGTCAGCACAAAATAA4330.11422451316087981No Hit
TTTTTAGGCTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATT4320.11396071520900712No Hit
GTTTGTAATCAGTTCCTTGTCTGATTAGTTCCTGGTCCCCAAAATTTCCT4300.11343311930526172No Hit
AAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTT4300.11343311930526172No Hit
GTCTGTATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTT4290.113169321353389No Hit
GTACACATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCAT4270.11264172544964361No Hit
ATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAG4270.11264172544964361No Hit
GCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGC4260.11237792749777091No Hit
CTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGT4240.11185033159402549No Hit
CATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTG4230.11158653364215279No Hit
GTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGC4210.1110589377384074No Hit
CACCTGTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATG4200.1107951397865347No Hit
TTCTTGAACTGTTGCGACTACGTGATGAGGAACGAGAAGAGGCTTGACTG4200.1107951397865347No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC4170.1100037459309166No Hit
ATATAGCCCATCTGCCTTGTGTGGTCTGTATGAGTTTAGGCCTGAGTTGA4140.1092123520752985No Hit
ACATCTACTTGTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAG4130.10894855412342577No Hit
GGCTTGAGTTTCATCAGCCTTCTTCTTTTTGTCCTTTTTAGGCTCTGTTG4130.10894855412342577No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG4110.10842095821968038No Hit
TGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTT4100.10815716026780768No Hit
GACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCT4070.10736576641218958No Hit
CACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTA4060.10710196846031687No Hit
TTCTCACATAGTGCATCAACAGCGGCATGAGAGCAAGCTGTATACACTAT4050.10683817050844417No Hit
GTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAATGAC4020.10604677665282607No Hit
GTTCCCCTACTGCTGCCTGGAGTTGAATTTCTTGAACTGTTGCGACTACG4020.10604677665282607No Hit
CACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAA4000.10551918074908066No Hit
GGTTCAATCTGTCAAGCAGCAGCAAAGCAAGAGCAGCATCACCGCCATTG3960.10446398894158986No Hit
GTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATG3960.10446398894158986No Hit
GTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGT3920.10340879713409905No Hit
GTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGG3900.10288120123035364No Hit
TTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAA3880.10235360532660825No Hit
GCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCA3860.10182600942286284No Hit
GCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAAT3850.10156221147099014No Hit
GCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCT3840.10129841351911745No Hit
ATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAA3820.10077081761537203No Hit
ATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTA3800.10024322171162663No Hit
GCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCAC3800.10024322171162663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAG151.2294076E-4145.05
GAAATCC100.007089138145.08
TCGCATC100.007089138145.02
GTCGCAT100.007089138145.01
CGCATCA100.007089138145.03
GACCGCG151.2294076E-4145.07
GGTCAGC151.2294076E-4145.06
ATACTAC202.1288834E-6145.06
GCTCTAA100.007089138145.01
ACTAGAC100.007089138145.08
TGCGATG100.007089138145.08
CCGAGAT100.007089138145.06
TTGCGTC100.007089138145.07
TTGCGAT100.007089138145.07
TTACGGT151.2294076E-4145.04
GGACCGC151.2294076E-4145.06
TCCCCAT100.007089138145.02
CGGTATT151.2294076E-4145.07
CAATAAC151.2294076E-4145.01
AGGGAAA100.007089138145.05
Produced by FastQC (version 0.11.5)