FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_19_C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_19_C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582135
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGAAATCAAT40620.6977762890051277No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT30690.5271972995954547No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA25720.4418219141608047No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT25110.4313432451235538No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC24670.4237848608999631No Hit
TATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAA24110.4141650991608476No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC21120.3628024427323559No Hit
CATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACC19920.3421886675771084No Hit
CATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACA18910.3248387401547751No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC18880.32432339577589386No Hit
GTAATAAGAACACCATTACGGGCATTTCTAAATAAGTCTACTTGACCATC18300.3143600711175243No Hit
TAATTGAACTGTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAG17250.29632301785668275No Hit
GCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATC16620.2855007859001778No Hit
TTTCTACTCTGAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATC16410.2818933752480095No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA15340.2635127590679138No Hit
CTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACG15320.2631691961486597No Hit
ACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGAC15300.26282563322940555No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT14730.25303409003066296No Hit
GTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAA14430.2478806462418511No Hit
GAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATT14100.24221185807415807No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG13680.23499703676982142No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC13460.23121784465802603No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT13410.23035893735989074No Hit
TCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCA13310.2286411227636201No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC13170.22623618232884124No Hit
ATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAG13070.22451836773257064No Hit
GTTTCAGGTAATTGTTGGACAACACCATCAACTTTCTTATAATAATTGAA12430.2135243543164386No Hit
CTCCTAGCACCATCATCATACACAGTTCTTGCTGTCATAAGGATTAGTAA11620.19961005608664656No Hit
GTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTG11360.19514373813634295No Hit
GAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATCAACTTTCTTA11210.192567016241937No Hit
GTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAAT11190.1922234533226829No Hit
GTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAA10910.18741357245312512No Hit
TTGTTGGACAACACCATCAACTTTCTTATAATAATTGAACTGTGTTTTTA10770.18500863201834625No Hit
CCATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAAC10500.1803705326084156No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA10340.17762202925438259No Hit
GTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAAT10190.17504530735997664No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC9810.16851761189414827No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC9740.16731514167675882No Hit
GTTGTAAACCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCA9620.16525376416123408No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG9460.16250526080720107No Hit
ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA9330.16027210183204926No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA9320.16010032037242222No Hit
TATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACT9240.1587260686954057No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT9190.1578671613972704No Hit
GTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAA9130.156836472639508No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC9120.15666469117988094No Hit
GTCTTAGGGTCGTACATATCACTAATAATGAGATCCCATTTATTAGCTGT9090.15614934680099976No Hit
GTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAGACTAGCTTGT9030.1551186580432374No Hit
GTAATAAATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCA9000.1546033136643562No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG8940.15357262490659382No Hit
ATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGACAA8860.15219837322957733No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG8850.1520265917699503No Hit
AGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAACACTACC8850.1520265917699503No Hit
TCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCA8810.15133946593144204No Hit
GTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGA8790.1509959030121879No Hit
GTATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGAC8780.1508241215525608No Hit
CCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCATTTCTAAA8740.1501369957140526No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT8600.1477320552792737No Hit
CTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCC8590.14756027381964665No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG8590.14756027381964665No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT8580.14738849236001958No Hit
ACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATG8430.14481177046561364No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG8380.14395286316747832No Hit
CCTTAAAACGTTTAGCTAGTCCAATCAGTAGATGTAAACCACCTAACTGA8310.1427503929500889No Hit
ATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCA8270.14206326711158063No Hit
AGCTTATAAAGATCAGCATTCCAAGAATGTTCTGTTATCTTTATAGCCAC8230.14137614127307238No Hit
ATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTG8160.14017367105568296No Hit
TTCCAAGAATGTTCTGTTATCTTTATAGCCACGGAACCTCCAAGAGCTAG7870.13519200872649814No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA7670.1317563795339569No Hit
CAGTAGATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGA7660.13158459807432984No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAATAACTCGTC7660.13158459807432984No Hit
GTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGC7630.13106925369544864No Hit
GAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTTTAGCTAGT7620.1308974722358216No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC7570.13003856493768626No Hit
ACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGC7440.12780540596253445No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT7400.1271182801240262No Hit
AGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTC7280.12505690260850147No Hit
TTCAAAAGGTGATTCCTTAAAACGTTTAGCTAGTCCAATCAGTAGATGTA7210.12385443239111202No Hit
TTTCTAAATAAGTCTACTTGACCATCAACTCTACCATCAAAAAAGACAGT7170.12316730655260377No Hit
TATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATT7170.12316730655260377No Hit
CCCTTACGAAGAATGGTTTTCAAGCCAGATTCATTATGGTATTCGGCAAG7160.12299552509297672No Hit
TTTATTAGCTGTATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTG7150.12282374363334965No Hit
GTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGAT7110.1221366177948414No Hit
AGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTCTAATA7060.12127771049670608No Hit
GCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAA7030.1207623661178249No Hit
CTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTA6940.11921633298118135No Hit
ACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATG6800.11681139254640245No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG6780.11646782962714834No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG6770.11629604816752127No Hit
CTTTATAGCCACGGAACCTCCAAGAGCTAGCTTTTGTTGTATAAACCCAC6750.11595248524826715No Hit
ATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGATATGTT6710.1152653594097589No Hit
GAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTT6660.11440645211162359No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT6640.11406288919236947No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC6640.11406288919236947No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT6630.1138911077327424No Hit
GTTCGAAGGCATAGCCTTCTAATTTATACCGTTCAATGAATTCATCCATA6620.11371932627311533No Hit
AATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATG6530.11217329313647179No Hit
GTTATGAAATAGTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAA6510.11182973021721765No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG6510.11182973021721765No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC6490.11148616729796353No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC6470.11114260437870939No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC6460.11097082291908235No Hit
GTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTAT6400.10994013416131997No Hit
AACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTT6390.10976835270169291No Hit
CATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAA6320.10856588248430347No Hit
ATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAG6230.1070198493476599No Hit
ATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAACAC6200.10650450496877872No Hit
TTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATTTGTGCACCAC6180.10616094204952459No Hit
CAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACA6170.10598916058989755No Hit
GCCTTCTAATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGA6130.10530203475138929No Hit
CCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTC6130.10530203475138929No Hit
CATTTGAGCTTTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAA6120.10513025329176222No Hit
GTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAA6070.1042713459936269No Hit
GGCATAGCCTTCTAATTTATACCGTTCAATGAATTCATCCATAGCTAATT6040.10375600161474571No Hit
GCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTC6000.10306887577623747No Hit
CTTTAGAGTCATTTTCTTTTGTAACATTTTTAGTCTTAGGGTCGTACATA5970.10255353139735628No Hit
CGTTTAGCTAGTCCAATCAGTAGATGTAAACCACCTAACTGACTATGACT5950.10220996847810217No Hit
GTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA5950.10220996847810217No Hit
GATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGATATGT5870.10083571680108566No Hit
CATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAACA5860.1006639353414586No Hit
TTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACA5860.1006639353414586No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG151.2297432E-4145.05
AACCGGT100.0070901075144.999987
AACCGGC100.0070901075144.999987
AGGAGTG100.0070901075144.999987
GGTCGAG100.0070901075144.999987
CGTGCCG100.0070901075144.999985
GCCGGAT100.0070901075144.999988
AGTGCCG100.0070901075144.999985
GTCGTAT100.0070901075144.999981
TCGTATA100.0070901075144.999982
GAACCGG100.0070901075144.999986
TAGTACG100.0070901075144.999987
GTGCCGG100.0070901075144.999986
ACGGTGA100.0070901075144.999986
CTCCTAG2200.0121.9318161
CCTTACG700.0103.571432
CCCTTAC700.0103.571431
TCCTAGC2600.0103.173082
TACACCG406.114242E-790.624995
TAGCACC3050.087.950825
Produced by FastQC (version 0.11.5)