FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_03_C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_03_C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521536
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGAAATCAAT37150.7123189961958523No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA30730.5892210700699473No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC24470.4691910050312922No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT22540.43218493066633945No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT21990.4216391581789177No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC17460.3347803411461529No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG17370.3330546692845748No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG16030.3073613326788563No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC14990.2874202356117315No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC14340.2749570499447785No Hit
TATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAA13910.26671217327279423No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC13230.25367376365198185No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT12640.24236102589274758No Hit
GAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATT12630.24216928457479445No Hit
CATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACA12090.23181525340532583No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA12020.23047306417965396No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT11910.2283639096821696No Hit
CATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACC11840.2270217204564977No Hit
GTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAAT11670.22376211805129465No Hit
GCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATC11320.21705117192293533No Hit
GTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAA11250.21570898269726346No Hit
TTTCTACTCTGAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATC11240.21551724137931033No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA10860.20823107129709167No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG10570.20267057307645112No Hit
GTATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGAC10520.2017118664866855No Hit
ATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTGCATCAGAGACAA10400.19941097067124802No Hit
CTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACG10400.19941097067124802No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT10170.19500092035832617No Hit
GTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAA10040.19250828322493557No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT9790.1877147502761075No Hit
GTCTTAGGGTCGTACATATCACTAATAATGAGATCCCATTTATTAGCTGT9730.18656430236838878No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC9690.18579733709657625No Hit
GTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAAT9500.18215425205546693No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT9160.17563504724506074No Hit
GTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGA9010.1727589274757639No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA8910.17084151429623268No Hit
TCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCA8890.17045803166032641No Hit
ACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGAC8860.16988280770646705No Hit
GTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGA8840.1694993250705608No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC8830.16930758375260768No Hit
GTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAG8760.16796539452693582No Hit
TCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCA8740.16758191189102958No Hit
GTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTG8680.16643146398331085No Hit
TAATTGAACTGTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAG8540.1637470855319671No Hit
ATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAG8480.16259663762424836No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG8260.15837832862927967No Hit
AGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTCTAATA8190.1570361394036078No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC8060.1545435022702172No Hit
TTGTTGGACAACACCATCAACTTTCTTATAATAATTGAACTGTGTTTTTA7960.15262608909068598No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT7920.15185912381887348No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC7860.15070867591115475No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC7830.15013345195729536No Hit
ACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATG7720.14802429745981102No Hit
TTTATTAGCTGTATGTACAGTTGCACAATCACCAATCAAAGTTGAATCTG7620.1461068842802798No Hit
CTTTATAGCCACGGAACCTCCAAGAGCTAGCTTTTGTTGTATAAACCCAC7390.14169683396735797No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG7330.1405463860596392No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG7300.13997116210577987No Hit
ATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCA7280.1395876794698736No Hit
GTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGT7280.1395876794698736No Hit
CCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTC7170.13747852497238924No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC7130.13671155970057677No Hit
TTCCAAGAATGTTCTGTTATCTTTATAGCCACGGAACCTCCAAGAGCTAG7040.13498588783899865No Hit
ATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTCTAATAGCATT7030.13479414652104554No Hit
CCCTTACGAAGAATGGTTTTCAAGCCAGATTCATTATGGTATTCGGCAAG6930.13287673334151429No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC6860.13153454411584242No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC6770.12980887225426432No Hit
GAGTAAAGTAAGTTTCAGGTAATTGTTGGACAACACCATCAACTTTCTTA6750.1294253896183581No Hit
GGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCAT6660.12769971775677996No Hit
CCATTAAGACTAGCTTGTTTGGGACCTACAGATGGTTGTAAACCTTTAAC6650.12750797643882686No Hit
CTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCC6630.12712449380292062No Hit
ATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTAT6550.12559056325929563No Hit
CTTTAGAGTCATTTTCTTTTGTAACATTTTTAGTCTTAGGGTCGTACATA6470.12405663271567062No Hit
GTTGTAAACCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCA6430.12328966744385814No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT6430.12328966744385814No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT6420.123097926125905No Hit
GTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACA6380.12233096085409252No Hit
AGCTTATAAAGATCAGCATTCCAAGAATGTTCTGTTATCTTTATAGCCAC6310.12098877162842066No Hit
CCTTTAACACTACCTTCTGTAATAAGAACACCATTACGGGCATTTCTAAA6300.12079703031046755No Hit
GTAATAAGAACACCATTACGGGCATTTCTAAATAAGTCTACTTGACCATC6280.12041354767456129No Hit
GAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTTTAGCTAGT6200.11887961713093631No Hit
AACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTT6150.1179209105411707No Hit
GTAATAAATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCA6110.1171539452693582No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG6090.11677046263345195No Hit
AGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTC6020.11542827340778009No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC6010.11523653208982698No Hit
GATTAGGCATAGCAACACCCGGTTGCCACGCTTGACTAGATTGTAATTTT5940.11389434286415512No Hit
CTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTA5940.11389434286415512No Hit
GTTCGAAGGCATAGCCTTCTAATTTATACCGTTCAATGAATTCATCCATA5900.1131273775923426No Hit
GTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACA5870.11255215363848325No Hit
GTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCATCTTGGACTG5830.11178518836667076No Hit
GAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACA5770.11063474045895202No Hit
ACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAAT5740.11005951650509264No Hit
CAGTAGATGTAAACCACCTAACTGACTATGACTAAAATCTCCATAAACGA5730.10986777518713951No Hit
GTGTTTTTACGGCTTCTCCAATTAATGTGACTCCATTAAGACTAGCTTGT5720.10967603386918641No Hit
GCTGTCCACCATGCGAAGTGTCCCATGAGCTTATAAAGATCAGCATTCCA5690.10910080991532703No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG5630.1079503620076083No Hit
CCTTAGAAACTACAGATAAATCTTGGGATTTTATTATTTCAACAAAATCA5630.1079503620076083No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACT5610.10756687937170203No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT5570.10679991409988955No Hit
GGCATAGCCTTCTAATTTATACCGTTCAATGAATTCATCCATAGCTAATT5520.10584120751012394No Hit
CCATAATTTTGAAGGTCACACTTTTCTAATAGCATTCTTTGCATTTTGTA5480.10507424223831145No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT5470.10488250092035832No Hit
CTCCAAGAGCTAGCTTTTGTTGTATAAACCCACAAATGTAAGTGAAAAAA5440.10430727696649897No Hit
GTTATGAAATAGTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAA5430.10411553564854584No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG5430.10411553564854584No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC5370.1029650877408271No Hit
ACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGC5350.10258160510492084No Hit
ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA5350.10258160510492084No Hit
CATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTT5300.10162289851515524No Hit
GTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTT5300.10162289851515524No Hit
GTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAA5230.10028070928948336No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATGC100.007089896145.01
CTCGGGT100.007089896145.08
TCGTCAA100.007089896145.06
ACCACGG100.007089896145.07
CGAGAAG100.007089896145.07
CGCACAT100.007089896145.07
CACCGTG100.007089896145.07
ACTTTCG400.0145.01
CCCGGGA100.007089896145.08
CCACGGG100.007089896145.08
ACGGTGG100.007089896145.09
CTCCTAG850.0136.470581
CCTTACG1050.0124.285712
CCCTTAC1050.0124.285711
TAGCACC1050.0110.476195
TACACCG203.8650085E-4108.755
ATAGGGA1650.0101.060611
TGTATCG301.598738E-596.6666645
TCATATA259.384212E-487.02
TAATCCA259.384212E-487.04
Produced by FastQC (version 0.11.5)