FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_02_D.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_02_D.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502906
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG73821.4678687468433465No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG36070.7172314508079045No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA35590.7076869236000366No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG32460.6454486524320648No Hit
GACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAG31680.6299387957192796No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA30490.6062763220164404No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC27050.5378738770267207No Hit
GTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAA25470.5064564749674889No Hit
AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC24060.4784194262943771No Hit
ATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAAT23010.4575407730271661No Hit
CGTTTATATAGCCCATCTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGA21790.43328176637383525No Hit
GCTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAATCTGCAGCAG20840.4143915562749301No Hit
GTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTTTAGTG20470.4070343165521986No Hit
GTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCTCAG19890.39550134617602495No Hit
AAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAAC19770.39311521437405794No Hit
ATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCT19140.3805880224137314No Hit
ATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGG18440.3666689202355908No Hit
CCATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCA17760.3531475066911113No Hit
GTGTTACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTA17430.3465856442357021No Hit
GCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTC16780.3336607636417144No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA16690.33187116479023915No Hit
CCCTAATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAAC16690.33187116479023915No Hit
ACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGAC16180.3217301046318795No Hit
GTTTTGTATGCGTCAATATGCTTATTCAGCAAAATGACTTGATCTTTGAA16070.3195428171467431No Hit
ACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAA15280.30383411611712724No Hit
CAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAA15110.3004537627310074No Hit
AGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTG14340.28514275033505265No Hit
AGTCAGCACTGCTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAA14000.27838204356281293No Hit
GTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCT13990.27818319924598234No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG13780.27400746859254016No Hit
TCAATATGCTTATTCAGCAAAATGACTTGATCTTTGAAATTTGGATCTTT13610.2706271152064203No Hit
GAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAA13540.2692352049886062No Hit
GTTACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGA13440.2672467618203004No Hit
CCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACG13160.26167912094904416No Hit
GTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTTGCAATTGTTT12980.2580999232460937No Hit
GACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCC12730.2531288153253292No Hit
ATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTGCCTTG12470.24795886308773407No Hit
ACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCA12470.24795886308773407No Hit
CTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTC12300.24457850970161424No Hit
GTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTT12080.24020393473134144No Hit
TACTTGTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTAT11930.23722126997888274No Hit
GTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTG11660.23185247342445708No Hit
GCACAAGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATAT11560.22986403025615126No Hit
ATTCAAGGCTCCCTCAGTTGCAACCCATATGATGCCGTCTTTGTTAGCAC11460.22787558708784544No Hit
ATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGC11340.22548945528587847No Hit
GTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTATATATC11100.22071719168194454No Hit
GATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGC10920.21713799397899408No Hit
GTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCT10850.21574608376118No Hit
TCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACAC10360.20600271223648156No Hit
CTCCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGG10360.20600271223648156No Hit
ACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGAT10270.20421311338500633No Hit
GTTGCGACTACGTGATGAGGAACGAGAAGAGGCTTGACTGCCGCCTCTGC10120.20123044863254763No Hit
AACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACC10120.20123044863254763No Hit
CACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCA10100.2008327599988865No Hit
ACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTGCC9870.1962593407117831No Hit
ACATCTACTTGTGCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAG9570.1902940112068657No Hit
GAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAG9530.1894986339395434No Hit
GCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACA9460.18810672372172932No Hit
ATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACAT9370.1863171248702541No Hit
TGCTTATTCAGCAAAATGACTTGATCTTTGAAATTTGGATCTTTGTCATC9330.18552174760293177No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC9260.18412983738511768No Hit
ATATAGCCCATCTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGA9130.18154486126632016No Hit
ATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTATATATCGTAAA9090.18074948399899782No Hit
GGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCAT9060.18015295104850607No Hit
GATTAGTTCCTGGTCCCCAAAATTTCCTTGGGTTTGTTCTGGACCACGTC9040.17975526241484494No Hit
CCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGA8980.17856219651386146No Hit
CATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTG8950.1779656635633697No Hit
TTACCAGACATTTTGCTCTCAAGCTGGTTCAATCTGTCAAGCAGCAGCAA8940.17776681924653912No Hit
CTTCTGCGTAGAAGCCTTTTGGCAATGTTGTTCCTTGAGGAAGTTGTAGC8880.17657375334555564No Hit
TTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAA8880.17657375334555564No Hit
GCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAA8840.17577837607823332No Hit
GTTCCTTGTCTGATTAGTTCCTGGTCCCCAAAATTTCCTTGGGTTTGTTC8770.17438646586041923No Hit
AGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTC8680.172596867008944No Hit
TTTTTAGGCTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATT8510.16921651362282414No Hit
GAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAAC8480.1686199806723324No Hit
TCTTAGAAGCCTCAGCAGCAGATTTCTTAGTGACAGTTTGGCCTTGTTGT8440.1678246034050101No Hit
CCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTC8360.16623384887036544No Hit
GAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACA8280.16464309433572077No Hit
ACATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGC8230.1636488727515679No Hit
GTTTGTAATCAGTTCCTTGTCTGATTAGTTCCTGGTCCCCAAAATTTCCT8140.16185927390009267No Hit
CACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATC8080.16066620799910916No Hit
TGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAA7910.15728585461298933No Hit
TTCTTGAACTGTTGCGACTACGTGATGAGGAACGAGAAGAGGCTTGACTG7870.15649047734566698No Hit
GCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTT7860.15629163302883642No Hit
CTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAATG7820.15549625576151407No Hit
GTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATG7800.15509856712785292No Hit
CTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCTCAGCAGCA7760.1543031898605306No Hit
ATATGATGCCGTCTTTGTTAGCACCATAGGGAAGTCCAGCTTCTGGCCCA7670.15251359100905537No Hit
CCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATT7650.1521159023753942No Hit
GTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTAC7610.15132052510807187No Hit
CACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTC7540.14992861489025783No Hit
GTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGA7510.14933208193976608No Hit
GCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGC7270.14455981833583215No Hit
GTTAGCAGGATTGCGGGTGCCAATGTGATCTTTTGGTGTATTCAAGGCTC7250.144162129702171No Hit
GTCAAGCAGCAGCAAAGCAAGAGCAGCATCACCGCCATTGCCAGCCATTC7230.14376444106850983No Hit
GGTTCAATCTGTCAAGCAGCAGCAAAGCAAGAGCAGCATCACCGCCATTG7210.14336675243484864No Hit
CACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAA7170.14257137516752633No Hit
ATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAG7160.14237253085069576No Hit
AATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAG7140.1419748422170346No Hit
GTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAATGAC7020.13958871041506762No Hit
CTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGT7020.13958871041506762No Hit
GTTGCAATTGTTTGGAGAAATCATCCAAATCTGCAGCAGGAAGAAGAGTC6890.13700373429627008No Hit
TTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCTC6870.13660604566260892No Hit
GTTCCCCTACTGCTGCCTGGAGTTGAATTTCTTGAACTGTTGCGACTACG6870.13660604566260892No Hit
GTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGT6860.13640720134577833No Hit
CTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCCATCTGCCT6820.135611824078456No Hit
GCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAAT6700.13322569227648906No Hit
GTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGT6680.13282800364282787No Hit
GCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTTGCAA6680.13282800364282787No Hit
GAGTTGAGTCAGCACTGCTCATGGATTGTTGCAATTGTTTGGAGAAATCA6460.1284534286725551No Hit
GGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCC6400.12726036277157163No Hit
GGCTTGAGTTTCATCAGCCTTCTTCTTTTTGTCCTTTTTAGGCTCTGTTG6390.12706151845474103No Hit
TCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATT6350.12626614118741872No Hit
GCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCA6330.12586845255375756No Hit
GATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAA6330.12586845255375756No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG6310.1254707639200964No Hit
GCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAAT6290.12507307528643524No Hit
CCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTC6290.12507307528643524No Hit
GTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTT6220.12368116506862117No Hit
ATTGTATGCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCT6170.12268694348446826No Hit
CTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAG6170.12268694348446826No Hit
ATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTT6160.12248809916763768No Hit
ATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAG6090.12109618894982363No Hit
GTATACACTATGCGAGCAGAAGGGTAGTAGAGAGCTAGGCCAATAGCAAA6090.12109618894982363No Hit
GGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAAT6080.12089734463299304No Hit
ACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCT6070.12069850031616247No Hit
AATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTG6060.12049965599933189No Hit
GATATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTG6020.11970427873200956No Hit
GCTCCCTTCTGCGTAGAAGCCTTTTGGCAATGTTGTTCCTTGAGGAAGTT6010.11950543441517898No Hit
TGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTT5990.11910774578151781No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT5960.11851121283102607No Hit
CACCTGTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATG5930.11791467988053433No Hit
ATTCCGAAGAACGCTGAAGCGCTGGGGGCAAATTGTGCAATTTGCGGCCA5910.11751699124687316No Hit
GCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCAC5880.11692045829638142No Hit
TCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGG5850.11632392534588969No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATATCAGATGAGTTT5810.11552854807856737No Hit
GCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCT5760.11453432649441446No Hit
TTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGT5750.11433548217758388No Hit
ATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTG5710.11354010491026155No Hit
CTTAGTGACAGTTTGGCCTTGTTGTTGTTGGCCTTTACCAGACATTTTGC5700.11334126059343097No Hit
GGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGC5670.11274472764293923No Hit
GTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGAT5640.1121481946924475No Hit
TATATAGCCCATCTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTG5620.11175050605878632No Hit
CTTAGAAGCCTCAGCAGCAGATTTCTTAGTGACAGTTTGGCCTTGTTGTT5590.11115397310829458No Hit
ATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAA5580.110955128791464No Hit
GTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAAT5570.11075628447463344No Hit
GAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGT5550.11035859584097227No Hit
CCATAAGACAGTTTAAATGTCTCCTCAGTAGCTTTGAGCGTTTCTGCTGC5520.10976206289048053No Hit
GCTTTAGTGGCAGTACGTTTTTGCCGAGGCTTCTTAGAAGCCTCAGCAGC5510.10956321857364995No Hit
TCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGG5480.10896668562315821No Hit
ATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGG5470.10876784130632762No Hit
CATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTC5440.10817130835583588No Hit
CCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAA5380.10697824245485239No Hit
GGTTTGTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTT5370.10677939813802183No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC5250.10439326633605486No Hit
GAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGC5230.10399557770239369No Hit
GCTATGTAGTTACGAGAATTCATTCTGCACAAGAGTAGACTATATATCGT5220.10379673338556311No Hit
GTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTG5160.10260366748457961No Hit
GGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGACATTC5140.10220597885091846No Hit
GAAGAAGAGTCACAGTTTGCTGTTTCTTCTGTCTCTGCGGTAAGGCTTGA5120.1018082902172573No Hit
GAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTG5110.10160944590042673No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATATT100.007089821145.09
AGCGTAC100.007089821145.08
GTCGCTA100.007089821145.02
GGTACCC100.007089821145.05
CCTAGTA151.2296444E-4145.02
TAAAGCG100.007089821145.05
CCGATAA100.007089821145.09
CCCTAGT151.2296444E-4145.01
GAACTAT100.007089821145.01
ATTACCG100.007089821145.05
TCGATTG100.007089821145.02
TAACGCC100.007089821145.04
ATATCGA151.2296444E-4145.03
CCCGTAT100.007089821145.08
ATCCAGT100.007089821145.06
ACAGACC202.1295673E-6145.08
CTCGATT100.007089821145.01
CTATTCA151.2296444E-4145.04
TATAGGG100.007089821145.02
ACCCGTA100.007089821145.07
Produced by FastQC (version 0.11.5)