FastQCFastQC Report
Wed 8 Apr 2020
000000000-CP3RN_l01_n01_CV_02_C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CP3RN_l01_n01_CV_02_C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198490
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGCACCATGGCACAGAGAGGTTAAGTAACATGCCCGAAGCTACAC5470.27558063378507736No Hit
GTGTAGGACTGGGGCACCAAGAGAAAGGAGCAGAGAGAGGGTATCTGAAT4310.21713940248879035No Hit
AATTTATACCGTTCAATGAATTCATCCATAGCTAATTCTAAGAAATCAAT3450.17381228273464658No Hit
CCCTTATCCTCAGTCTGACTAACTTCTGCTCATCTTCCACACCTCCACCA3280.1652476195274321No Hit
GTCCTACACACTGCCCCGGGACCCTCCCAAATGTCCCACACAGGCCTGGG2740.13804221875157438No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA2320.11688246259257394No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT2080.10479117335885939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCG554.049629E-665.9090966
TAGCACC1550.056.129035
TCCTAGC1750.049.7142832
TCCTAAT752.5431358E-548.3333321
CCTAATA951.9130566E-645.7894742
TACCCGC855.3259708E-542.6470577
CTAGCAC2050.042.4390264
ACCAGTA750.001480519938.6666647
CCTAGCA2401.8189894E-1236.253
GCACCAT2650.035.5660367
ATAGGGA1352.133614E-532.222221
TATACCG1801.2835517E-732.222225
TATTATC1153.1530415E-431.521742
GTATTAT2151.7949787E-830.3488371
ATACCGT2151.7949787E-830.3488376
AGCACCA2901.2732926E-1130.0000026
TATTGGG1255.139148E-429.05
CCCTTAT3550.028.5915471
GTGGTAT1050.00769381527.6190471
GTATACA1358.059782E-426.8518521
Produced by FastQC (version 0.11.5)