FastQCFastQC Report
Fri 31 Jan 2020
000000000-CHT9C_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CHT9C_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967833
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC11530.11913212300055899No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT400.006050281754.3762366
GCAGGTA1151.4116995E-744.133729
CGAAAGC1002.733621E-643.500993
CAATTCG951.02841535E-438.1607329
GCTAAAC800.00203635536.2508247
GCAGGGT1551.46409E-632.7443739
TACAATA900.003627768732.2229587
GAGCAAA5400.032.2229541
GCAAAAG5750.031.5224593
TACAATC2558.913048E-1131.2752257
AGCAGGG3250.031.2330958
TAGGTTA1402.746158E-531.0721366
TCTAGCT1306.481933E-427.8852522
AGCTCTA1409.993031E-425.8934465
AGCAAAA7050.025.7098062
GTATAGG1450.001226251525.0005723
CTCTATG1751.2554902E-424.857717
TTTAAAC1500.001494039824.1672173
GAAAGCA2101.5547268E-524.1672174
TAAGGAG1500.001494039824.1672175
Produced by FastQC (version 0.11.5)