FastQCFastQC Report
Fri 31 Jan 2020
000000000-CHT9C_l01_n02_NEGATIVE_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CHT9C_l01_n02_NEGATIVE_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6790
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGCATTCGGCCAGCACTGAAAATACCGCTATTACAGCCAGACAT280.4123711340206186No Hit
CTTCTATATTGGTGCTATTTTTGTAATTGTCACTGTTTTTCCGTGGGATC270.39764359351988215No Hit
GTATACGTACACAAATATCTTTGGAGGCGCAATATAGTTTAAAACAACAC250.3681885125184094No Hit
GTCGTAGGGATGTCAGAAATTATTGCGGTTGGAGCGTATATGAAGTACTG240.35346097201767307No Hit
ATATAAAGGCACACCATTACGAGAAATCTTCGTAAAGAATTTTGGCGCTT230.3387334315169367No Hit
GTATATGCTCAACAGCTGGTTTTCCTTCCAAAGACTTAAGCTTTAGCTTG220.3240058910162003No Hit
ATCCAGCACCATTCAATGGACGGGTCCGTCTGTACTGCTTGCTTATGCAA200.29455081001472755No Hit
AAATAATACTTTGGATCGCGCTTGTTAACGTTTTTTGCGGATTTTTCGCT200.29455081001472755No Hit
GTATTAATTGGTATGTGGTTTAATGATGAAACACGTATTTCGCTTATTGT190.27982326951399117No Hit
TACGTATACAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCAT190.27982326951399117No Hit
GCTTTATACATTGGGAGTGAATGGTCAAGCGCCAAAATTCTTTACGAAGA190.27982326951399117No Hit
GCTTAGGATCTCACTCCCGGCGGCACGAACGGTCCTTCTTAGCAAATTTG180.2650957290132548No Hit
ATGAAAAGGCGTGCAAAACTGAAATTTCGCACGCCTTGAACCAAAAATAA170.2503681885125184No Hit
GGGTAGCACCGGTGATCAAAGCAGTTGCAATGATAGGCACGGATTGTTCC170.2503681885125184No Hit
GTATGATCCCATGGTTTGTCATCCTTATTAGTCAAATTCGATTCAGAAGA170.2503681885125184No Hit
ATGTGAAACAAGCCATCAACTATCATTGCTTGGCTTGTCATGAGGAAGAG160.23564064801178203No Hit
CTTAAGAAGACCGTTAAAAAAATAGGGGAACAATCCGTGCCTATCATTGC160.23564064801178203No Hit
GTATTAAGAAAAACCAGGCATAACCCGCCCGGTCCTTTGTCATGATGAGA160.23564064801178203No Hit
GTATACTGCCGGATCGGTCGAAATCAGGCTGCGGCTATGTGCTGGAAAAG150.22091310751104565No Hit
ATATAGAAGATTAATATACGCCAAATAATACTTTGGATCGCGCTTGTTAA150.22091310751104565No Hit
TCTTAATACTCTCTAACCCATTCGTCATCATATCTTTTCCGGCTGCTATG140.2061855670103093No Hit
GCATAATAACTAATAATCACGATGCCCAGGAAAACAATCCCTACAATAAG140.2061855670103093No Hit
GTATGTGAAACAAGCCATCAACTATCATTGCTTGGCTTGTCATGAGGAAG140.2061855670103093No Hit
AATTAATACCATCATTAAGAAGGCAATCGTCAAATAGTTTGTAACAGGAG140.2061855670103093No Hit
CTCTAACCCATTCGTCATCATATCTTTTCCGGCTGCTATGGCACCACTCC140.2061855670103093No Hit
GGACTAAAGATTCTTGATGAAGCGGTAAGTGCCGCCCCTCAGGATAGCAG130.1914580265095729No Hit
AGCATATACTGATCCCCGAGAGCCTCACATCAAATTTGCTAAGAAGGACC130.1914580265095729No Hit
ATCTAACCTTTGGGCACATGGCGGCTTTTTTACAGGTGGCTGGTCAGGAT130.1914580265095729No Hit
ACCCTGAGGAGTGGTGCCATAGCAGCCGGAAAAGATATGATGACGAATGG130.1914580265095729No Hit
GTCATGATGAGAGGACATCCGAAAGTTTGTATTCCTGGCATGAATGCCAT130.1914580265095729No Hit
AAATAAGGCGATGCTATCCAAATAGAATCTTTTGCTGATGTGATCATCGA130.1914580265095729No Hit
CCCTACGACAATCCACTGAAACCAGTTACTCCATGCAGTTAAATAACCTG130.1914580265095729No Hit
ATCCTAAGCTGGGCGGAGCAGCAAAAGAAAGAAGAAACTCCTCGCAAACT130.1914580265095729No Hit
CACTTGCACTGTATACGTACACAAATATCTTTGGAGGCGCAATATAGTTT120.17673048600883653No Hit
CTCTTGTTATTGCCGCTTATCAAGGGGTCGAACTCATCGGAATTACAGCT120.17673048600883653No Hit
TTCCAGCACATAGCCGCAGCCTGATTTCGACCGATCCGGCAGTATACCTC120.17673048600883653No Hit
GGGTAGAAGCGATTTTTCCCAATAGGACTTTAGGAGACTGATTTTCATCA120.17673048600883653No Hit
AAGAAGGACCGTTCGTGCCGCCGGGAGTGAGATCCTAAGCTGGGCGGAGC120.17673048600883653No Hit
TTATTATGCTTTGGGAATAAGCAAGCGTGTTCCATTAGATGTACAAACAG120.17673048600883653No Hit
GTTTGTAACAGGAGCAAATGGCATTTTGAATGGATGTTTGTCCATTTCAG120.17673048600883653No Hit
CTTCTTAAGAGCATCTTTTGACATGCGTGTCACCTCCTTTCAAAAGTAGC120.17673048600883653No Hit
GGGTTAGAGAGTATTAAGAAAAACCAGGCATAACCCGCCCGGTCCTTTGT120.17673048600883653No Hit
GTACGTATACAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCA110.16200294550810015No Hit
CATCAATACAATTGTTACAATTTTAATCATCGCAAACCAAAATTCAAATT110.16200294550810015No Hit
GTGCTACCCGGGTCAGCAATGAAATGAATGATGCGGTGAGTGAATCGGTG110.16200294550810015No Hit
TTCACATACTGCGCTAGCTTCCTCAAATATTTGTTTAGGATAAAGTGTGG110.16200294550810015No Hit
TGTAATACCAGTCCTGAAGAAAAATCATCTGCAGCGTACGGACAGCTTCT110.16200294550810015No Hit
CTCCCAATGTATAAAGCATTCGGCCAGCACTGAAAATACCGCTATTACAG110.16200294550810015No Hit
GACTTAAGCTTTAGCTTGAGGAGATGCAGAAAATGAGGATTCTGCGTTTC110.16200294550810015No Hit
GAATACTTCAATGGCATTCATGCCAGGAATACAAACTTTCGGATGTCCTC110.16200294550810015No Hit
GTTTAAAACAACACCAATTGCTAAACCAATTAGTACTGCAATCGTTCCAT110.16200294550810015No Hit
GCATATACTGATCCCCGAGAGCCTCACATCAAATTTGCTAAGAAGGACCG110.16200294550810015No Hit
CATTGAATTCATCCATGATGATTTCATGTGATTTTTTTATATCCCGCTCA110.16200294550810015No Hit
CCTCCATCCATGATGTCAAAATCTCGGTCGGGATCATAAGGAATTTCCTC110.16200294550810015No Hit
CAATTTAACTGTGAAAAATGTATCCAGAATACTTCAATGGCATTCATGCC110.16200294550810015No Hit
TTTTTATTATGCGTGCAATGGGAGAAATGTTGTATTTAGAGCCAAGTACA110.16200294550810015No Hit
ATGTGAGACTGCTCGTCCCCAAAAATCCTGATAAAAAAATCGTATTCTAC100.14727540500736377No Hit
ATATTAATCTTCTATATTGGTGCTATTTTTGTAATTGTCACTGTTTTTCC100.14727540500736377No Hit
TCATTAAACCACATACCAATTAATACCATCATTAAGAAGGCAATCGTCAA100.14727540500736377No Hit
TATCTAACCTTTGGGCACATGGCGGCTTTTTTACAGGTGGCTGGTCAGGA100.14727540500736377No Hit
ATACTGATCCCCGAGAGCCTCACATCAAATTTGCTAAGAAGGACCGTTCG100.14727540500736377No Hit
CCATTACGAGAAATCTTCGTAAAGAATTTTGGCGCTTGACCATTCACTCC100.14727540500736377No Hit
CTTCTACCCTGAGGAGTGGTGCCATAGCAGCCGGAAAAGATATGATGACG100.14727540500736377No Hit
ATCCAGAATACTTCAATGGCATTCATGCCAGGAATACAAACTTTCGGATG100.14727540500736377No Hit
ATTTTAATCATCGCAAACCAAAATTCAAATTCACCAAATGATTTAACAGA100.14727540500736377No Hit
CCTTGAACCAAAAATAAAATAACTTTATTGATTTTCTATTGCTTACTCTG100.14727540500736377No Hit
GTAGAAACAAGGGGAGGAAATGGAATGGCAAACAAAGAATTAACGAGAGG100.14727540500736377No Hit
CCCTATGGCCTGTTGCAGCATGGACTAGCGCTTTTTCTTCTGATTGAGTT100.14727540500736377No Hit
ACAAGAGATAGTGCAAAGAAAAATCCTGACCAGCCACCTGTAAAAAAGCC100.14727540500736377No Hit
CCTTTATATGGAACGATTGCAGTACTAATTGGTTTAGCAATTGGTGTTGT90.1325478645066274No Hit
GGAACAATCCGTGCCTATCATTGCAACTGCTTTGATCACCGGTGCTACCC90.1325478645066274No Hit
CTATTTGACGATTGCCTTCTTAATGATGGTATTAATTGGTATGTGGTTTA90.1325478645066274No Hit
CTGCAATAATCATCAATACAATTGTTACAATTTTAATCATCGCAAACCAA90.1325478645066274No Hit
GTATCAACATTCGCGAAAATTGGGATTACGGCAGCAGCCGGCATTATTAA90.1325478645066274No Hit
GGTATTACATGACCGGTGAAAACTTGGTGAAACAAGAATATATGACTCCT90.1325478645066274No Hit
CATTGGGAGTGAATGGTCAAGCGCCAAAATTCTTTACGAAGATTTCTCGT90.1325478645066274No Hit
TGTCAAAAGATGCTCTTAAGAAGACCGTTAAAAAAATAGGGGAACAATCC90.1325478645066274No Hit
AAATAAGACCAATCCCTGCAATAATCATCAATACAATTGTTACAATTTTA90.1325478645066274No Hit
GTACATACATCCATTAGCAGGTTATTTAACTGCATGGAGTAACTGGTTTC90.1325478645066274No Hit
CCTGGTATCCATGCAGGCAAATCCGGGAACCAGTACTTCATATACGCTCC90.1325478645066274No Hit
ATTCATTTCATTGCTGACCCGGGTAGCACCGGTGATCAAAGCAGTTGCAA90.1325478645066274No Hit
AGCTTGAGGAGATGCAGAAAATGAGGATTCTGCGTTTCGTTCATCAGCCT90.1325478645066274No Hit
TCTTAAGAGCATCTTTTGACATGCGTGTCACCTCCTTTCAAAAGTAGCTT90.1325478645066274No Hit
GCTTTAGCTTGAGGAGATGCAGAAAATGAGGATTCTGCGTTTCGTTCATC90.1325478645066274No Hit
GTCTTATTTTCTTCGGATTTGGAAACGCAGGAGACGCAACCGGATTATCT90.1325478645066274No Hit
CGTATACAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCATCC90.1325478645066274No Hit
GTCATGAGGAAGAGGAAATTCCTTATGATCCCGACCGAGATTTTGACATC90.1325478645066274No Hit
ATTACAGCCAGACATCGCTGCTGTAATTACGACAAAGTTAATAATGCCGG80.11782032400589101No Hit
TGTATGTACTTATGGCCAAATGTTGCAAATGAACCTGTACTTGGCTCTAA80.11782032400589101No Hit
TAACAAGAGATAGTGCAAAGAAAAATCCTGACCAGCCACCTGTAAAAAAG80.11782032400589101No Hit
CTGTATACGTACACAAATATCTTTGGAGGCGCAATATAGTTTAAAACAAC80.11782032400589101No Hit
ATTGCTTACTCTGCAGCTAATCCAAGTGAATTATCGGTTAGTCACTTCAT80.11782032400589101No Hit
GTTTTAAACTATATTGCGCCTCCAAAGATATTTGTGTACGTATACAGTGC80.11782032400589101No Hit
GCTTAAGTCTTTGGAAGGAAAACCAGCTGTTGAGCATATACTGATCCCCG80.11782032400589101No Hit
GGATAGCAGGATCCGGCTCCTGCGAGGCAGGGCCTCATATAGGTTGCCTG80.11782032400589101No Hit
ATCCGTGCCTATCATTGCAACTGCTTTGATCACCGGTGCTACCCGGGTCA80.11782032400589101No Hit
CTGCCGGATCGGTCGAAATCAGGCTGCGGCTATGTGCTGGAAAAGGCTGA80.11782032400589101No Hit
CATATAGGTTGCCTGAACAGCATTTCCAACTGGCCCAAACGGTGATTGAA80.11782032400589101No Hit
CGCTTGCTTATTCCCAAAGCATAATAACTAATAATCACGATGCCCAGGAA80.11782032400589101No Hit
CTGTAATTCCGATGAGTTCGACCCCTTGATAAGCGGCAATAACAAGAGAT80.11782032400589101No Hit
GTCCTTCTTAGCAAATTTGATGTGAGGCTCTCGGGGATCAGTATATGCTC80.11782032400589101No Hit
TAAGAAGACCGTTAAAAAAATAGGGGAACAATCCGTGCCTATCATTGCAA80.11782032400589101No Hit
TAAGTACATACATCCATTAGCAGGTTATTTAACTGCATGGAGTAACTGGT80.11782032400589101No Hit
GTATTATTTGGCGTATATTAATCTTCTATATTGGTGCTATTTTTGTAATT80.11782032400589101No Hit
ATCTAATGGAACACGCTTGCTTATTCCCAAAGCATAATAACTAATAATCA80.11782032400589101No Hit
CTTAATGATGGTATTAATTGGTATGTGGTTTAATGATGAAACACGTATTT80.11782032400589101No Hit
GATTAATATACGCCAAATAATACTTTGGATCGCGCTTGTTAACGTTTTTT80.11782032400589101No Hit
GTCCTTTGTCATGATGAGAGGACATCCGAAAGTTTGTATTCCTGGCATGA80.11782032400589101No Hit
CCGTAATGTATTCAAAACTCTACTTATTCACTGATAAAATGCATATTTTC80.11782032400589101No Hit
CTTTATTTCAGTATCCAAAACCTTTTATCTTTTATTATTTCCTTTCCAAA80.11782032400589101No Hit
GATTACACCTTTCTCATCGATCGGGAAATGCATGAAGAAGGCTTCCTTGA80.11782032400589101No Hit
GACTTTAGGAGACTGATTTTCATCACTCACCGATTCACTCACCGCATCAT80.11782032400589101No Hit
CCTCAAGCTAAAGCTTAAGTCTTTGGAAGGAAAACCAGCTGTTGAGCATA80.11782032400589101No Hit
GTGCTGGAAAAGGCTGATGAACGAAACGCAGAATCCTCATTTTCTGCATC80.11782032400589101No Hit
AAATACAACATTTCTCCCATTGCACGCATAATAAAAAAGATAAATATTCC80.11782032400589101No Hit
GCTAATGGATGTATGTACTTATGGCCAAATGTTGCAAATGAACCTGTACT80.11782032400589101No Hit
CACTTTATCCTAAACAAATATTTGAGGAAGCTAGCGCAGTATGTGAAACA80.11782032400589101No Hit
CCATTAGATGTACAAACAGATGATGAAGTGACTAACCGATAATTCACTTG80.11782032400589101No Hit
ATAGATATCTCCGAAAATATGCATTTTATCAGTGAATAAGTAGAGTTTTG80.11782032400589101No Hit
CAATTTTACTGTGCATAAATCCATCTTTGTACTCATAAATTTTCACGCCT80.11782032400589101No Hit
CCTCATGACAAGCCAAGCAATGATAGTTGATGGCTTGTTTCACATACTGC70.10309278350515465No Hit
GGTTAGATAATCCGGTTGCGTCTCCTGCGTTTCCAAATCCGAAGAAAATA70.10309278350515465No Hit
GCCTCATATAGGTTGCCTGAACAGCATTTCCAACTGGCCCAAACGGTGAT70.10309278350515465No Hit
AGCTAGCGCAGTATGTGAAACAAGCCATCAACTATCATTGCTTGGCTTGT70.10309278350515465No Hit
GTATATGAAGTACTGGTTCCCGGATTTGCCTGCATGGATACCAGGTATCA70.10309278350515465No Hit
GGGAGAAATGTTGTATTTAGAGCCAAGTACAGGTTCATTTGCAACATTTG70.10309278350515465No Hit
GCAGTATACCTCGCCAAGTTCGTAGATAAGCTGCAAGTAATTTTCGGTCT70.10309278350515465No Hit
CATCATGACAAAGGACCGGGCGGGTTATGCCTGGTTTTTCTTAATACTCT70.10309278350515465No Hit
GCCATCAACTATCATTGCTTGGCTTGTCATGAGGAAGAGGAAATTCCTTA70.10309278350515465No Hit
TTTATATCCCGCTCAAATTCATCGACTAGAGTTTGTGTACTTGTTGTCCG70.10309278350515465No Hit
GTTACAAACTATTTGACGATTGCCTTCTTAATGATGGTATTAATTGGTAT70.10309278350515465No Hit
ATATATGACTCCTTCAATCGAGCTTGAAGAACATGAAGGCGGCGTTCAGA70.10309278350515465No Hit
GTTTGTACATCTAATGGAACACGCTTGCTTATTCCCAAAGCATAATAACT70.10309278350515465No Hit
CGTCTGTACTGCTTGCTTATGCAATCTGCGGAATATTTATCTTTTTTATT70.10309278350515465No Hit
GTGAAAAATGTATCCAGAATACTTCAATGGCATTCATGCCAGGAATACAA70.10309278350515465No Hit
TATATGAAGTACTGGTTCCCGGATTTGCCTGCATGGATACCAGGTATCAT70.10309278350515465No Hit
TCGCAATGGTGATTCTTGGTGCAGCAAACTTAATTTCTGTTAAATCATTT70.10309278350515465No Hit
GTTTTCCTGGGCATCGTGATTATTAGTTATTATGCTTTGGGAATAAGCAA70.10309278350515465No Hit
GTATTACATGACCGGTGAAAACTTGGTGAAACAAGAATATATGACTCCTT70.10309278350515465No Hit
AATCTGAACGCCGCCTTCATGTTCTTCAAGCTCGATTGAAGGAGTCATAT70.10309278350515465No Hit
ATATTTATCTTTTTTATTATGCGTGCAATGGGAGAAATGTTGTATTTAGA70.10309278350515465No Hit
ACTTTGGATCGCGCTTGTTAACGTTTTTTGCGGATTTTTCGCTTCTCCAG70.10309278350515465No Hit
CAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCATCCTTATTA70.10309278350515465No Hit
TCTACGAACTTGGCGAGGTATACTGCCGGATCGGTCGAAATCAGGCTGCG70.10309278350515465No Hit
GCGCTAGTCCATGCTGCAACAGGCCATAGGGAGGGAAAGCTACTTTTGAA70.10309278350515465No Hit
GTCTTCTTAAGAGCATCTTTTGACATGCGTGTCACCTCCTTTCAAAAGTA70.10309278350515465No Hit
GTTGTATTTAGAGCCAAGTACAGGTTCATTTGCAACATTTGGCCATAAGT70.10309278350515465No Hit
GATTGAGTTTGCGAGGAGTTTCTTCTTTCTTTTGCTGCTCCGCCCAGCTT70.10309278350515465No Hit
GAAAAATCCTGACCAGCCACCTGTAAAAAAGCCGCCATGTGCCCAAAGGT70.10309278350515465No Hit
TCTTTGTACTCATAAATTTTCACGCCTGCTTCAAGCAATTCAAGAAAATA70.10309278350515465No Hit
GTGCCATAGCAGCCGGAAAAGATATGATGACGAATGGGTTAGAGAGTATT70.10309278350515465No Hit
ATATTGGTGCTATTTTTGTAATTGTCACTGTTTTTCCGTGGGATCAGCTT70.10309278350515465No Hit
ATTTATGGGATCATCCAGCACCATTCAATGGACGGGTCCGTCTGTACTGC70.10309278350515465No Hit
CTTCAATGGCATTCATGCCAGGAATACAAACTTTCGGATGTCCTCTCATC70.10309278350515465No Hit
AGCTAAAGCTTAAGTCTTTGGAAGGAAAACCAGCTGTTGAGCATATACTG70.10309278350515465No Hit
GAGTAAGCAATAGAAAATCAATAAAGTTATTTTATTTTTGGTTCAAGGCG70.10309278350515465No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCCA100.0069366856145.04
TTTACAC100.0069366856145.0145
Produced by FastQC (version 0.11.5)