FastQCFastQC Report
Fri 31 Jan 2020
000000000-CHT9C_l01_n02_A_Texas_50_2012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CHT9C_l01_n02_A_Texas_50_2012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413313
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG13850.3350971297781584No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG12170.2944499689097609No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA9800.23710843839898577No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG8720.21097812069787303No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG8430.20396164650035203No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT7550.1826702765216676No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC7460.18049275004657486No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7320.17710548664087508No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG6890.16670174903765428No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC6690.16186280131522598No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTAGTGTGTG6550.15847553790952618No Hit
CACTAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA5870.14202311565327003No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA5840.14129727349490578No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT5710.1381519574753274No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC5440.13161937805004922No Hit
GTACATGATAGGACCCCTTATCGGACTCTATTGATGAATGAGTTGGGTGT5320.12871600941659228No Hit
GGCCTAGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC5250.12702237771374236No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA5210.12605458816925671No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT5080.12290927214967833No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT4940.11952200874397853No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA4940.11952200874397853No Hit
ATCATGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC4930.11928006135785711No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC4820.11661864011052157No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG4640.11226358716033612No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA4630.1120216397742147No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT4560.11032800807136481No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT4510.10911827114075774No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC4460.10790853421015066No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTCTTAAGGCCGTG4240.10258569171547956No Hit
GCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA4140.10016621785426541No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT100.007089368145.01
CCTACCA100.007089368145.09
ACCTACC100.007089368145.08
CGTACTC100.007089368145.03
GCAGGTT100.007089368145.07
TCGTACT100.007089368145.02
TATAGTA100.007089368145.02
GCAGGGT801.8189894E-1281.56259
CAGTACG1150.069.347839
AGGGTGT1700.068.235296
AAGGTAC451.19303295E-464.444449
TAATTGG350.003565023462.1428572
TATATTC2650.060.1886753
TTATATT2650.060.1886752
AGGTCGT753.7557766E-758.06
CGAAAGC2050.056.5853653
TAAACTA1450.055.05
GGTCGTT805.8652404E-754.3757
CAAGCTA1353.6379788E-1253.70374
ACAAGGT2050.053.048787
Produced by FastQC (version 0.11.5)