FastQCFastQC Report
Fri 31 Jan 2020
000000000-CHT9C_l01_n02_A_Texas_50_2012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CHT9C_l01_n02_A_Texas_50_2012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446538
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG17410.3898884305479041No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG15230.34106839731445027No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA12280.2750045908746848No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG10470.23447052658452358No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG10080.22573666742808002No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC9190.20580555294286265No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT7940.17781241462092812No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC7900.17691663419462622No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA7620.17064617121051287No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTAGTGTGTG7350.16459965333297502No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC7240.16213625716064478No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG7150.1601207512014655No Hit
GTACATGATAGGACCCCTTATCGGACTCTATTGATGAATGAGTTGGGTGT6830.15295450779105024No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6660.14914744097926716No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA6370.14265303288857833No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT6340.14198119756885194No Hit
CACTAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA6240.13974174650309715No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC6080.13615862479788954No Hit
ATCATGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC6070.13593467969131406No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA5870.13145577755980456No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT5780.12944027160062527No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT5580.12496136946911574No Hit
GGCCTAGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC5540.12406558904281384No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA5400.12093035755075716No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTCTTAAGGCCGTG5230.11712329073897407No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG5200.11645145541924763No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC5190.11622751031267214No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT5090.1139880592469174No Hit
GCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA5080.11376411414034192No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA5000.11197255328773811No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC4940.11062888264828526No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG4870.10906126690225693No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC4640.10391052945102096No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT4640.10391052945102096No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT4630.1036865843444455No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTAGTGT4500.1007752979589643No Hit
GAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACTCGCGAGATA4500.1007752979589643No Hit
GCCTTAAGAGAGGCCCTTCTACGGAAGGAGTACCTGAGTCTATGAGGGAA4500.1007752979589643No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTAGT4500.1007752979589643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG1450.085.09
GCAGGGT1050.082.857149
ACAAGGT2500.055.17
TAGAAAC4900.054.7448962
GCAGGGG953.1341187E-853.4210479
TATATTC3100.051.4516143
TAAACTA1850.050.9459425
TTATATT3100.049.1129042
GCTTATA1351.9099389E-1048.3333362
GTAAAGA752.5482956E-548.3333363
AGTAAAG752.5482956E-548.3333362
AAGGTAC450.0096356748.3333329
TAGGCAT1255.185939E-946.3999989
TCTATGC3800.045.789473
GAATGTC803.7313115E-545.31257
CAAAAGC10150.045.04
CAAGCTA1151.4102261E-744.1304364
AAGCTAA1151.4102261E-744.1304365
CGAAAGC1505.347829E-1043.53
TTTAAAC2000.043.53
Produced by FastQC (version 0.11.5)