FastQCFastQC Report
Fri 31 Jan 2020
000000000-CHT9C_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CHT9C_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967833
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG369193.8146043790612634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT1101.6752892E-952.7270134
GCAGGTA1550.051.4513559
GTACTAC604.9451244E-448.333091
AGCACCG951.9208856E-645.7892465
CGAAAGC951.9208856E-645.7892464
GCACCGT951.9208856E-645.7892466
GAGCGAA1002.7341448E-643.4997831
ACTCAGC1002.7341448E-643.4997831
TAGCCCA855.341522E-542.6468476
CACCGTC1053.8241233E-641.4283687
TCTACAC1755.2750693E-1141.4283643
GTCATAC1252.7189162E-740.59981
GTATAGC800.00203663336.249821
TATGTGT800.00203663336.249825
GAGCAAA5800.033.7498321
TAGGTTA1301.654783E-533.461376
TCTAGCT1102.4361632E-432.9543842
ACAAGGG2008.951247E-932.624843
ATATACT900.00362826232.222065
CTCTATG1402.7466793E-531.0712722
Produced by FastQC (version 0.11.5)