FastQCFastQC Report
Fri 31 Jan 2020
000000000-CHT9C_l01_n01_A_Texas_50_2012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CHT9C_l01_n01_A_Texas_50_2012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413313
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG13320.3222739183137235No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG11460.2772717044951405No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA11090.2683196512086482No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG9310.22525301647903645No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG8310.2010582778668951No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT8140.196945172302831No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC7840.1896867507191886No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA6970.16863732812662557No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC6670.16137890654298317No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA6390.15460437973158359No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTAGTGTGTG6380.15436243234546215No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG6130.1483137476924268No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA5840.14129727349490578No Hit
GTACATGATAGGACCCCTTATCGGACTCTATTGATGAATGAGTTGGGTGT5730.13863585224757025No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT5700.137910010089206No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC5560.1345227466835062No Hit
CACTAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA5470.13234522020841347No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA5330.12895795680271369No Hit
GGCCTAGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC5290.127990167258228No Hit
ATCATGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC5250.12702237771374236No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT5230.12653848294149955No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA5230.12653848294149955No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT4820.11661864011052157No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC4760.11516695579379307No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT4760.11516695579379307No Hit
GCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA4710.11395721886318601No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC4580.11081190284360763No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG4540.10984411329912197No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC4540.10984411329912197No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT4510.10911827114075774No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA4500.10887632375463632No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC4380.10597295512117935No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT4350.1052471129628151No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC4350.1052471129628151No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTAGT4290.10379542864608664No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT4260.10306958648772238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA202.1291125E-6145.04
ATGAGGT256.460352E-6116.03
GAGGTAA256.460352E-6116.05
TAATTGG300.001934935872.52
AGGGTGT1750.070.428576
CAGTACG1250.063.89
TTTAAAC1750.062.1428573
TAAACTA1800.060.4166685
GCAGGGG1200.060.4166689
CGAAAGC2650.060.1886753
AGTAGAA3650.055.6164361
GCAGGGT858.9119567E-751.1764689
GCGAAAG3200.049.843752
TAGAAAC4150.048.915663
TATATTC2750.047.4545483
TTATATT2750.047.4545482
GAGCGAA2450.047.346941
GTATTAA4200.046.6071431
ATTAATT4300.045.523263
GGTGTTT2800.044.0178578
Produced by FastQC (version 0.11.5)