FastQCFastQC Report
Fri 31 Jan 2020
000000000-CHT9C_l01_n01_A_Texas_50_2012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CHT9C_l01_n01_A_Texas_50_2012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446538
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG17760.39772650927804576No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA13990.31329920409909123No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG13910.31150764324648744No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG11340.25395375085659005No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG9300.2082689491151929No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC9290.20804500400861742No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT8840.19796747421272098No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTAGTGTGTG8830.19774352910614548No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC8490.19012939548257932No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG8170.18296315207216407No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA7720.17288562227626764No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA7710.17266167716969216No Hit
CACTAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA7570.1695264456776355No Hit
GTACATGATAGGACCCCTTATCGGACTCTATTGATGAATGAGTTGGGTGT7020.1572094648159843No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA6990.15653762949625788No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC6900.1545221235370786No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC6630.1484756056595407No Hit
GGCCTAGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC6620.14825166055296526No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA6530.14623615459378597No Hit
ATCATGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC6420.14377275842145573No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA6180.1383980758636443No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT5920.1325755030926819No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC5910.13235155798610646No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT5750.12876843628089882No Hit
GCCATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA5570.12473742436254025No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT5560.12451347925596479No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTAGT5550.1242895341493893No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT5540.12406558904281384No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG5490.12294586350993644No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG5460.12227402819021001No Hit
GCCTTAAGAGAGGCCCTTCTACGGAAGGAGTACCTGAGTCTATGAGGGAA5020.11242044350088906No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC5000.11197255328773811No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC4940.11062888264828526No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA4940.11062888264828526No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTCTTAAGGCCGTG4930.11040493754170977No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTAGTGT4870.10906126690225693No Hit
GAATAAAAGAACTACGGAATCTGATGTCGCAGTCTCGCACTCGCGAGATA4860.10883732179568144No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG4710.1054781451970493No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT4690.10503025498389836No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG4610.10323869413129452No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA4580.10256685881156812No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA4580.10256685881156812No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC4530.10144713327869073No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA4500.1007752979589643No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT4490.10055135285238882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAA1050.075.952381
GTCTGAT300.001935038272.51
GTTAATG1100.072.53
TAATGTA1100.072.55
GAGGGCC300.001935038272.59
AGGGTGT1550.070.161296
TACTGAT1700.063.970597
GTACTGA1700.063.970596
AGGGTGA808.072675E-963.43759
CAGGGTG808.072675E-963.43758
GCAGGGT808.072675E-963.43757
CAGAGGG350.003565210862.1428577
TACATTG502.009034E-458.06
AGAGGGC651.0926589E-555.7692348
TTTAAAC1700.055.4411773
TAAACTA1700.055.4411775
AGTAGAA3700.054.864861
CGAAAGC2200.052.7272723
TAATGGG1550.051.4516144
TAGTTTT1152.4792826E-950.4347849
Produced by FastQC (version 0.11.5)