FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224667
Sequences flagged as poor quality0
Sequence length251
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG250651.1266854769725088No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG181760.817021154177232No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG139410.6266555848583181No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG127030.5710068068614315No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGCTAACTTATCACTTTCGCTTGG89980.40446502779966614No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG83790.37664063880122284No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG63110.2836829062506883No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG32550.1463140326170164No Hit
CAACCTTGCGGTCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG28880.12981718162763237No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG25970.11673657226002813No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG24250.1090050780633686No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATAACTTATCGCTTTCGCTTGG24090.10828586930088863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCG252050.0248.754848
CGACCGT246250.0248.619899
AGTCTTG66750.0243.809342
GCGGCCG104050.0242.851448
CGGCCGT101950.0242.656639
TGCGGCC105000.0242.456447
TAGTCTT57600.0239.688781
TGCGAGC44550.0239.56567
GTCTTGC67700.0239.264453
TAATCTT153300.0230.992771
CAACCTT66600.0229.468351
TGCGATC12250.0216.264397
CGATCGT12200.0214.048579
TTGCGGT10000.0213.200646
ATCTTGC197200.0210.022493
TCTTGCG329950.0208.8364
GCGATCG13050.0201.073418
GCCGGCG8100.0200.912288
TGCGACC316000.0199.531287
GAGACCG7300.0198.736118
Produced by FastQC (version 0.11.5)