FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n02_EMP_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n02_EMP_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67518
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG683410.121745312361147No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG29034.29959418229213No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG23003.406499007672028No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTCACGCGTTAGCTGCG18172.6911342160609024No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTATCGCGTTAGCTACG15522.2986462869160817No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG13762.0379750585029175No Hit
CAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCG10681.581800408779881No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTTAG7431.1004472881305727No Hit
CACTCTTGCGAGCGTACTCCCCAGGTGGATAACTTAACGCTTTCGCTAAG6851.0145442696762346No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGGGCACTTCACGGTTTCCCTGCG6410.9493764625729435No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCTGACTTCACGCGTTAGCTACG5940.8797653958944283No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTATCGCTTTCGCTTGG5380.7968245504902396No Hit
CATTCTTGCGAACGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG5110.7568352143132202No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTTG4870.7212891377114251No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG4200.6220563405314138No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTATCGCGTTAGCTTCG3340.49468289937498144No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGATTACTTAACGCTTTCGCTTAG3330.4932018128499067No Hit
CAGCCTTGCGACCATACTCCCCAGGCGGGGCACTTCACGGTTTCCCTTCG3260.48283420717438313No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTACCGCGTTAGCTACG3170.46950442844870993No Hit
TAATCTTGCGATCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG2990.4428448709973637No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG2960.4384016114221393No Hit
TAGCCTTGCGGCCGTACTTCCCAGGCGGAGAACTTAATGCGTTAGCTACG2900.4295150922716905No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGTAAACTTAACGCTTTCGCTTGG2870.42507183269646615No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGTCTACTTATCGCGTTAGCTGCG2870.42507183269646615No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGAAGACTTAATGCGTTAGCTGCG2670.39545010219497023No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTGG2590.38360140999437187No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTCCG2370.3510175064427264No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2370.3510175064427264No Hit
TAGCCTTGCGGCCGTACTTCCCAGGCGGAGAACTTAATGCGTTAGCTGCG2290.339168814242128No Hit
CAACCTTGCAGCTGTACTCCTCAGGCGGGGAGCTTAACGCGTTAGCTTTA2240.33176338161675406No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG2240.33176338161675406No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAATGCTTTCGCTCAG2220.32880120856660444No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGAAGCTTAATGCGTTAGCTGCG2210.32732012204152966No Hit
CATCCTTGCGGACGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG2190.3243579489913801No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATGCTCATAGGGTTACCTGCG2160.3199146894161557No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG2070.30658491069048255No Hit
CACCGTTGCCGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTTG2020.2991794780651086No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATTACTTATCACTTTCGCTTAG1930.2858496993394354No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1880.2784442667140614No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTAGTGCGTTAGCTGCG1710.2532657957877899No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1700.2517847092627151No Hit
CACACTTGCGTGCGTACTCCCCAGGTGGGATACTTATCACTTTCGCTTAG1700.2517847092627151No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCGACG1670.24734144968749072No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTTCG1620.23993601706211676No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAACGTGTTAACTTCG1580.2340116709618176No Hit
TAGTCTTGCGACCGTACTCCCCAGGCGGGGCGCTTAATGCGTTAGCTGCG1520.22512515181136883No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAATTTCACGCGTTAGCTACG1510.223644065286294No Hit
CAACCTTGCGGTCGTACTTCCCAGGTGGATTACTTAACGCTTTAGCTTGG1480.21920080571106965No Hit
CAACCTTGCGATCGTACTCCCCAGGTGGATCACTTAATGCTTTCGCTTGG1440.21327645961077046No Hit
CAACCTTGCGATCGTACTCCCCAGGTGCAATACTTATCACTTTCGCTTAG1400.20735211351047125No Hit
TAGTCTTGCGACCGTAGTCCTCAGGCGGCACACTTAACGCGTTAGCTTGG1360.20142776741017213No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCG1350.19994668088509732No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTTCG1340.1984655943600225No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTAATGCGTTAGCTGCG1320.19550342130987292No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAATGCTTAATGCGTTAGCTGCG1270.18809798868449895No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG1240.18365472910927455No Hit
AAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCG1200.1777303830089754No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTCCG1180.1747682099588258No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGCACACTTAACGCGTTAGCTCCG1150.1703249503836014No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTCGCTGCG1140.1688438638585266No Hit
CATCCTTGCGGACGTACTCCCCAGGTGGGATACTTATCGGTTTCCCTTGG1140.1688438638585266No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGATAACTTAATGCGTTAGCTGCG1130.1673627773334518No Hit
TAGCCTTGCGGCCGTAGTCCTCAGGCGGCATACTTAACGCGTTAGCTTGG1110.16440060428330222No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTACG1090.16143843123315266No Hit
AAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTATCGCGTTAGCTACG1080.15995734470807785No Hit
TAGCCTTGCGGCCGTACTCCCCAGGCGGATAACTTATCGCGTGTGCTTCG1070.15847625818300307No Hit
CATTCTTGCGAACGTACTTCCCAGGTGGAATACTTAACGCTTTCGCTTGG1020.15107082555762907No Hit
CAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTTGATTTCTCAT960.1421843064071803No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTACTGCGTTAGCTGCG960.1421843064071803No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG950.14070321988210552No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGAAGGCTTAACGCGTTAGCTAAT920.13625996030688112No Hit
CAATCTTGCGATCGTACTTCCCAGGTGGGATACTTAATGCTTTCGCTCAG920.13625996030688112No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTATCGCGTTGGCTTCG910.13477887378180634No Hit
CACCCTTGCGAGCATACTTCCCAGGCGGGGTACTTAACGCGTTAGCTACG900.13329778725673155No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGGGTGCTTAACGCGTTAGCTGCG900.13329778725673155No Hit
TAATCTTGCGACCGTACTTCCCAGGCGGATCACTTAACGCGTTAGCTTCG890.13181670073165674No Hit
TAACCTTGCGGCCGTACTCCCCAGGCGGTCGACTTCACGCGTTAGCTGCG890.13181670073165674No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATTACTTAATGCGTTAGCTGCG870.12885452768150715No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGTGCTTATCGCGTTAACTACG860.12737344115643234No Hit
CAACNTTGNGGCNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTTCG860.12737344115643234No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGATTACTTAACGCTTTCGCTTTG840.12441126810628278No Hit
CACACTTGCGTGCGTACTCCCCAGGCGGAATGCTTATTGCGTTAACTGCA830.12293018158120797No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCT810.11996800853105838No Hit
TAATCTTGCGACCGTACTCCCCAGTCGGTCAACTTCACGCGTTAGCTGCG800.11848692200598358No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAACGCGTTAGCTGCG770.11404366243075921No Hit
CACCCTTGCGGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTAG760.11256257590568441No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGCGCACTTATCGGGTTTCCTTCG740.10960040285553482No Hit
TAGCCTTGCGGCCGTAGTCCTCAGGCGGCACACTTAACGCGTTAGCTTGG740.10960040285553482No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGACAACTTAATGCGTTAGCTGCG730.10811931633046004No Hit
TAATNTTGNGACNGTACTCCCCAGGCGGTCAACTTCACGCGTTAGCTGCG730.10811931633046004No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGTCAACTTATCGCGTTAGCTGCG720.10663822980538523No Hit
CAACCTTGCGGCCGTACTCCCCAGGCGGTGCGCTTACCGCGTTGGCTACG710.10515714328031044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG100.001358416251.719515
GCGAACG1100.0251.719518
CGGCCGG350.0251.719519
AACCATG100.001358416251.719512
ACTCTTG1500.0251.719512
AGTGCGA100.001358416251.719515
TGCGATC1900.0251.719517
AGCCTTG4450.0251.719512
TTGCCGG1350.0251.719516
GCAGCTG301.4551915E-11251.719518
ATTGCGG201.3549106E-7251.719515
GTGCGAG100.001358416251.719516
CCTTGAG100.001358416251.719514
ATCATGC650.0251.71953
CGACCGT29800.0250.87489
GCGACCG30800.0250.084998
CAACCTT11300.0249.491911
TTGCGAC31750.0249.341066
ATCTTGC26250.0249.322193
TGCGACC32700.0249.025287
Produced by FastQC (version 0.11.5)